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分泌型ルシフェラーゼレポーターアッセイ

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  • Small noncoding differentially methylated copy-number variants, including lncRNA genes, cause a lethal lung developmental disorder.
    Szafranski, P. et al.
    Genome Res. (2013) 23:23-33.
  • Outer Membrane Protein A (OmpA) of Shigella flexneri 2a Links Innate and Adaptive Immunity in a TLR2-dependent Manner and Involvement of IL-12 and Nitric Oxide.
    Pore, D. et al.
    J. Biol. Chem. (2012) 287:12589-12601.
  • 家族性筋萎縮性側索硬化症(fALS)の進行を決定する「SOD1」の発現レベルを抑制する低分子化合物をReady-To-Glowシステムでスクリーニングした例
    Chemical Library Screening Identifies a Small Molecule That Downregulates SOD1 Transcription for Drugs to Treat Amyotrophic Lateral Sclerosis
    Murakami, G et al. J. Biomol. Screen (2011) 16 (4), 405-414.
  • 漢方薬由来の化合物Sparstolonin BがNFκBシグナル伝達に影響を与えるかを解析した例
    Characterization of Sparstolonin B, a Chinese Herb-derived Compound, as a Selective Toll-like Receptor Antagonist with Potent Anti-inflammatory Properties
    Liang, Q et al. The Journal of Biological Chemistry (2011) 286, 26470-26479.
  • Metridiaルシフェラーゼを分泌型タンパク質(PZ)に融合して発現させ、その挙動を調べた例
    Unique secretion mode of human protein Z: its Gla domain is responsible for inefficient, vitamin K–dependent and warfarin-sensitive secretion
    Souri, M et al.
    Blood, (2009);113:3857-3864.
  • pMetLuc-reporter vectorにNox1転写調節領域をクローニングしLPS刺激に対する応答を調べた例
    Calcium-Independent Phospholipase A2β-Akt Signaling Is Involved in Lipopolysaccharide-Induced NADPH Oxidase 1 Expression and Foam Cell Formation
    Lee, S-H. et al.
    J. Immunol. (2009) Dec;183:7497-7504.
  • ADAMTS1 Is a Unique Hypoxic Early Response Gene Expressed by Endothelial Cells.
    Hatipoglu, O. F. et al.
    J. Biol. Chem. (2009) 284:16325-16333.
  • The soluble terminal complement complex (SC5b-9) up-regulates osteoprotegerin expression and release by endothelial cells: implications in rheumatoid arthritis.
    Corallini, F. et al.
    Rheumatology. (2009) 48:293-298.
  • Rhesus monkeys and humans share common susceptibility genes for age-related macular disease.
    Francis, P. J. et al.
    Hum. Mol. Genet. (2008) 17:2673-2680.
  • Identification and characterization of the human Gb3/CD77 synthase gene promoter.
    Okuda, T. and Nakayama, K.
    Glycobiology. (2008) 18:1028-1035.

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