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Protocols

Ingenio® Electroporation Kit & Solution

推奨のパルス条件およびプログラム選択

推奨のパルス条件(Gene Pulser Xcell Electroporator等での減衰波によるエレクトロポレーションに適用)
Cell Line Cuvette Size
(cm)
Cell Density
(× 106)cells/ml
DNA
(μg)
Electroporation
Volume(μl)
Voltage Capacitance
HL-60 0.2 10 2 100 150 950 μF
0.4 5 250 275 950 μF
Jurkat E6-1 0.2 10 2 100 150 950 μF
0.4 5 250 260 950 μF
K562 0.2 10 2 100 130 950 μF
0.4 5 250 250 950 μF
THP-1 0.2 10 2 100 140 950 μF
0.4 5 250 250 950 μF
RAW 264.7 0.2 5 2 100 150 950 μF
0.4 5 250 260 950 μF
CHO-K1 0.2 5 2 100 150 950 μF
0.4 5 250 280 950 μF
HUV-EC 0.2 3 2 100 not-tested 950 μF
0.4 5 250 250 950 μF
HeLa 0.2 3 2 100 130 950 μF
0.4 5 250 260 950 μF
BHK-21 0.2 10 2 100 150 950 μF
0.4 5 250 280 950 μF
SK-N-MC 0.2 5 2 100 90 950 μF
0.4 5 250 240 950 μF
SH-SY5Y 0.2 5 2 100 not-tested 950 μF
0.4 5 250 250 950 μF
U-937 0.2 10 2 100 not-tested 950 μF
0.4 5 250 260 950 μF
HEK-293 0.2 5 2 100 160 950 μF
0.4 5 250 250 950 μF
COS-7 0.2 5 2 100 150 950 μF
0.4 5 250 260 950 μF
A-549 0.2 5 2 100 not-tested 950 μF
0.4 5 250 280 950 μF
NIH-3T3 0.2 10 2 100 160 950 μF
0.4 5 250 260 950 μF
MEF 0.2 5 2 100 150 950 μF
0.4 5 250 230 950 μF
MCF-7 0.2 3 2 100 150 950 μF
0.4 5 250 not-tested 950 μF
PC-12 0.2 3 2 100 130 950 μF
0.4 5 250 240 950 μF
Vero 0.2 5 2 100 170 950 μF
0.4 5 250 not-tested 950 μF
HepG2 0.2 5 2 100 170 950 μF
0.4 5 250 not-tested 950 μF
Other (starting point) 0.2 5-10 2 100 80-160 800-1000 μF
0.4 5 250 200-300 800-1000 μF


推奨のプログラム選択(amaxa Nucleofector II 装置に適用)
Cell Line Program Setting Cell Density
(× 106)cells/ml
DNA
(μg)
Results Comparable to
HL-60 T-019 10 2 amaxa Solution V
Jurkat E6-1 X-001 10 2 amaxa Solution V
K562 T-016 10 2 amaxa Solution V
THP-1 V-001 10 2 amaxa Solution V
RAW 264.7 D-032 5 2 amaxa Solution V
CHO-K1 U-023 5 2 amaxa Solution T
HUV-EC V-001 3 2 amaxa Solution V
HeLa I-013 3 2 amaxa Solution R
BHK-21 A-031 10 2 amaxa Solution L
SK-N-MC S-020 5 2 amaxa Solution V
SH-SY5Y G-004 5 2 amaxa Solution V
U-937 W-001 10 2 amaxa Solution C
HEK-293 Q-001 5 2 amaxa Solution V
COS-7 W-001 5 2 amaxa Solution R
A-549 X-001 5 2 amaxa Solution T
NIH-3T3 U-030 10 2 amaxa Solution R
MCF-7 P-020 3 2 amaxa Solution V
Gene Pulser Xcell はBio-Rad社のトレードマークです。
Amaxa, NucleofectorはLONZA社又はその系列会社の登録商標です。
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