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Application

PA-Sugar Chain 058
(Oligomannose type, M4B)

TaKaRaハイマンノース型PA-Sugar ChainシリーズのHPLC2次元マップ

試料1:TaKaRaハイマンノース型 PA-Sugar Chainシリーズ
M5A=PA-Sugar Chain 017 M6B=PA-Sugar Chain 018
M6C=PA-Sugar Chain 051
M7A=PA-Sugar Chain 052
M7B=PA-Sugar Chain 053
M7D=PA-Sugar Chain 054
M8A=PA-Sugar Chain 019
M8B=PA-Sugar Chain 055
M8C=PA-Sugar Chain 056
M9A=PA-Sugar Chain 020

分析条件A 分析条件B
Column: PALPAK Type N (4.6 mmφ×250 mm) Column: PALPAK Type R (4.6 mmφ×250 mm)
Solvent A: Acetonitrile/H2O/500 mM Acetic acid-Triethylamine, pH7.3 (75/15/10, v/v/v) Solvent A: 100 mM Acetic acid-Triethylamine, pH4.0
Solvent B: Acetonitrile/H2O/500 mM Acetic acid-Triethylamine, pH7.3 (50/40/10, v/v/v) Solvent B: Solvent A containing 0.5% 1-Butanol
Eluent: 50% B, Isocratic Eluent: 5% B, Isocratic
Flow Rate: 1.0 ml/min Flow Rate: 1.0 ml/min
Detection: Fluorescence (Ex.: 320 nm, Em.: 400 nm) Detection: Fluorescence (Ex.: 320 nm, Em.: 400 nm)
Column Temp.: 40℃ Column Temp.: 40℃
Injection: 10 pmol each Injection: 10 pmol each

HPLCチャート1




試料2:TaKaRaハイマンノース型 PA-Sugar Chainシリーズ
M2B=PA-Sugar Chain 057(販売終了) M3B=PA-Sugar Chain 016 M4B=PA-Sugar Chain 058
M5A=PA-Sugar Chain 017


分析条件A 分析条件B
Column: PALPAK Type N (4.6 mmφ×250 mm) Column: PALPAK Type R (4.6 mmφ×250 mm)
Solvent A: Acetonitrile/H2O/500 mM Acetic acid-Triethylamine, pH7.3 (75/15/10, v/v/v) Solvent A: 100 mM Acetic acid-Triethylamine, pH4.0
Solvent B: Acetonitrile/H2O/500 mM Acetic acid-Triethylamine, pH7.3 (50/40/10, v/v/v) Solvent B: Solvent A containing 0.5% 1-Butanol
Eluent: 25% B, Isocratic Eluent: 5% B, Isocratic
Flow Rate: 1.0 ml/min Flow Rate: 1.0 ml/min
Detection: Fluorescence (Ex.: 310 nm, Em.: 380 nm) Detection: Fluorescence (Ex.: 320 nm, Em.: 400 nm)
Column Temp.: 40℃ Column Temp.: 40℃
Injection: 10 pmol each Injection: 10 pmol each

HPLCチャート2
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