Description

Premix Taq™ are optimized mixture composed of enzyme (TaKaRa Taq™ or TaKaRa Ex Taq™ ), reaction buffer and dNTP mixture as 2-fold concentrations. Premix Taq™ are designed to allow the user for quick and easy preparation (dilution only) of reaction mixture.

Storage

Shipped and stored at –20°C. Repetition of freeze-thawing and vigorous stirring should be avoided. Once it thawed, dispense into PCR tubes and store at –20°C.

Contents

Premix Taq™ (TaKaRa Taq™ Version 2.0)

TaKaRa Taq™	1.25 units/25 µl
dNTP Mixture (2× conc.)	ea. 0.4 mM

PCR buffer (2× conc.; 20 mM Tris-HCl, pH8.3, 100 mM KCl, 3 mM MgCl₂)

The composition of the reagent has been changed for better performance since lot.4201A.

PCR performance test

TaKaRa Taq™
Good performance of DNA amplification by PCR is confirmed by using λDNA as the template (amplified fragment: 8 kbp). Good performance of DNA amplification of a single copy gene by PCR is also confirmed by using human genome DNA as the template, (amplified fragment: 2.9 kbp).

PCR products

As most PCR products amplified with Premix Taq™ have one A added at 3′-terminus, the obtained PCR product can be directly used for cloning into T-vector. Also it is possible to clone the product in blunt-end vectors after blunting and phosphorylation of the end.

General reaction mixture for PCR (total 50 µl)

Premix Taq™	25 µl
Template	< 500 ng
Primer 1	0.2-1.0 µM
Primer 2	0.2-1.0 µM
Sterilized distilled water	up to 50 µl