Description

In order to maintain consumer trust in the safety of food products, a high priority has been placed on assuring product quality at each step of the food supply process. PCR (Polymerase Chain Reaction) process is recognized as one of the more useful methods for such food-related quality control applications.
PCR comprises a technique in which minute amounts of DNA can be used as a template for amplifying only the desired DNA fragments. A single cycle of this method comprises three incubation steps; DNA Thermal denaturation, primer annealing and DNA polymerase chain reaction. Amplification by a factor of 1 million can be achieved in relatively short periods of time by repeating this process.
Bacterial Screening PCR kit is designed to use the 16S rRNA gene as a target and employs the ENT primer for detecting strains in the Enterobacteriaceae family, including E. coli and salmonella bacteria and the BS primer for detecting genus Bacillus stains, including cereus bacteria, and genus Staphylococcus, including Staphylococcus aureus strains.
When used in combination with an enrichment culture, Bacteria Screening PCR Kit can be used to rapidly detect the presence of even minute numbers of bacteria in samples.

Storage

The 10% Chelex^® solution would be transported at room temparature but must be stored at 4°C.
All other components must be both transported and stored at -20°C.

Kit components

1. 2 × Premix Solution*1	2 × conc.	250 µl × 10 tubes (100 reactions)
2. Primer Mix ENT	5 µM each	125 µl (50 reactions)
3. Primer Mix BS	5 µM each	125 µl (50 reactions)
4. Positive Control ENT		25 µl (10 reactions)
5. Positive Control BS		25 µl (10 reactions)
6. dH2O		1 ml × 3 tubes
7. 10% Chelex® Solution*2		12 ml × 2 vials

^*1 Contents of 2 × Premix Solution

TaKaRa Ex Taq™	0.1 units/µl
Optimized Buffer (Mg2+ Concentration: 4 mM)	2 × conc.
dNTP Mixture	0.4 mM each

^*2 Chelex ^® is registered trademark of Bio-Rad Laboratories.

Target bacteria and amplified size

Primer	bacteria	Amplified size
ENT	Enterobacteriaceae (excluding Proteus mirabilis) and Vibrionaceae family	ca.420 bp
BS	genus Bacillus, genus Staphylococcus, genus Aerococcus bacteria	ca.380 bp