Description

This marker consists of 5 ssRNA fragments between 14 and 30 bases. (Applied volume: 2.5 µl per electrophoresis)
Fragment size (base): 30, 26, 22, 18, 14

Storage

-80°C

Form

RNase free water

RNA Loading Buffer supplied (Able to store at -20°C after opened)

95% Formamide
20 mM ETDA

6×Dye supplied (Able to store at -20°C or 4°C after opened)

0.5% Orange G
1 mM ETDA

Application

Used as a ssRNA molecular size marker shorter than 30 bases in gel electrophoresis.

Protocol

Add the same volume of RNA Loading Buffer to the ssRNA Ladder Marker to be just used. Then, add 6× Dye with 1/5 volume of the above mixture to apply on 15% aclylamide: bisacrylamide (19:1)/7 M Urea/ 0.5×TBE. Use 0.5× TBE buffer for electrophoresis.
Stain with SYBR^® Green II Nucleic Acid Stain (Cambrex).

Note

1. It is recommended to store in aliquots for several uses each after opened, not to repeat the excess freeze-thaw cycles.
2. Do not store ssRNA Ladder Maker after adding RNA Loading Buffer or 6× Dye.
3. Store at - 80°C quickly after used.
4. Extra precaution should be taken during the operation in order to prevent RNase contamination. Put on clean disposable gloves, and tubes and micropipette chips should be exclusively used for RNA experiments. Do not perform experiments which uses RNase in the same area.
5. Be sure to use this product on 15% acrylamide:bisacrylamide (19:1) /7 M Urea / 0.5× TBE gel and 0.5× TBE buffer. Under other conditions, the bands cannot be separated correctly.
6. Depending on the sequence of ssRNA sample and sample volume, the fragments may appear in a little difference lengths from this ladder marker.