E. coli JM109 Competent Cells
Provided with SOC medium
| Cat.# | Product | Size | Note |
|---|---|---|---|
| 9052 | E. coli JM109 Competent Cells | 100 µl × 10 |
Storage
-80°C
Genotype
E. coli JM109:
recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB)/F' [traD36, proAB+, lacIq, lacZΔM15]
Bacterial Strains
E. coli JM109:
JM109 is used to select the recombinant DNA using the activity of β-galactosidase (α -complementation) recovered with lacZα peptide of the vector DNA and lacZΔM15 coded in F' episome in this strain. This strain carries F' episome and it is useful for the preparation of ssDNA as well as gene library construction and subcloning.
Transformation Efficiency
When using 1 ng plasmid DNA for transformation
100 µl JM109 Competent Cells >1 × 108 cfu/µg pBR322
Note
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