Acc III (BspM II)
pKF3 Cloning Test- Provided with Loading Buffer
-
- RelatedInfo
- Instructions for the usage of restriction enzymes
- Restriction enzyme activity displaying system using universal and basal buffers
- Genome DNA analysis grade restriction enzyme
- pKF3 cloning test
- Effects of methylation on the activities of restriction enzymes
- Star activity of restriction enzymes
- Residual activities following various inactivation treatments
- Recommended Universal Buffer for Double Digestion
- Q&A
- Instructions for the usage of restriction enzymes
-
| Cat.# | Product | Size | Note |
|---|---|---|---|
| 1113A | Acc III (BspM II) | 20 units | |
| 1113B(A × 5) | Acc III (BspM II) | 100 units |
| Source: | Acinetobacter calcoaceticus |
| Concentration: | 1-5 units/µl |
| Supplied buffer: | Basal |
| Reaction temperature: | 60°C |
| Substrate for unit definition: | λDNA |
| Effect of DNA methylation: | This enzyme is cleavable TC5mCGGA, but its rate is about 1/75. Otherwise, enzyme activity is affected by dam methylase depending on the sequence following the recognition site. |
| Cleavage of pBR211: | The commercially available pBR322 is substantially not cleavable, becoause the adenine residues in the recognition site (TCCGGA*) are methylated by dam methylase. |
Note
- All products are intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc
- Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC.
- If you require licenses for other use, please call at +81 77 543 7247 or contact from our website at www.takara-bio.com
- Please confirm the content about the license or patent used in this document that relates to the Takara Bio product by clicking the license mark.
Moreover, please confirm the "Limited Use Label License" or "patent" concerning the product of another manufacturers or respective owners in their Web site/catalog etc.
