EcoR I
pKF3 Cloning Test- Provided with Loading Buffer
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- RelatedInfo
- Instructions for the usage of restriction enzymes
- Restriction enzyme activity displaying system using universal and basal buffers
- Genome DNA analysis grade restriction enzyme
- pKF3 cloning test
- Effects of methylation on the activities of restriction enzymes
- Star activity of restriction enzymes
- Residual activities following various inactivation treatments
- Recommended Universal Buffer for Double Digestion
- Q&A
- Instructions for the usage of restriction enzymes
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| Cat.# | Product | Size | Note |
|---|---|---|---|
| 1040A | EcoR I | 10,000 units | |
| 1040B(A × 5) | EcoR I | 50,000 units | |
| 1040AH | EcoR I (High concentration) | 10,000 units | |
| 1040BH(AH × 5) | EcoR I (High concentration) | 50,000 units | |
| 1040S | EcoR I | 5,000 units |
| Source: | Escherichia coli RY 13 |
| Concentration: | 8-20 units/µl [High concentration: 30-60 units/µl] |
| Supplied buffer: | H |
| Reaction temperature: | 37°C |
| Substrate for unit definition: | λDNA |
| Effect of DNA methylation: | Enzyme activity is affected by CG methylase depending on the sequence following the recognition site. |
| Star activity: | Unrelated site may often be cut in the presence of high concentration of glycerol or Mn2+, and at low ionic strength. Enzyme activity reduces to 70 to 80% by the addition of spermine (ca. 0.2mM), but star activity reduces to 30 to 50%. |
Note
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