Description

Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA, and produces mono- and oligonucleotides carrying a 5′-P terminus. More than 1000- fold amount of enzyme can degrade oligomer into all mononucleotides. An excess of the enzyme is required to degrade double-stranded DNA or RNA and DNA-RNA hybrids, and in this case, AT-rich regions are selectively degraded. This enzyme work well at A↓pN, T ↓pN sites, and especially A↓pN sites are 100% degraded. However, it is difficult to degrade C↓pC, C↓pG site.

Storage

-20°C

Concentration

10-50 units/µl

Form

10 mM Tris-HCl (pH7.5), 0.1 mM zinc acetate, 50% glycerol

Supplied buffer

• 10× Mung Bean Nuclease Buffer

300 mM sodium acetate (pH5.0), 1,000 mM NaCl, 10 mM zinc acetate, 50% glycerol

Source

Mung bean sprouts

Unit definition

One unit is the amount of the enzyme that converts 1 µg of heat-denatured calf thymus DNA into acid-soluble products at pH5.0 per minute at 37°C.

Purity

Exonuclease activity was not detected as judged from the intact gel elecrophoresis pattern.

Applications

• Mapping of hybridization (S1 mapping). It has less
   nibbling activity than S1 Nuclease (Cat.# 2410), extra
   bands are rarely generated.
  
• Making the ends of double-stranded DNA blunt-ended
   (however, the efficiency depends on the base sequence).
  

Note

Its ability to recognize double-stranded nucleic acids depends on the base sequence. It tends to cleave at ApN and at T(U) pN. It completely degrades ApA, but does not degrade G and C. Unlike S1 Nuclease, it does not cleave the strand opposite to that which has been nicked.