Pfu Protease S
| Cat.# | Product | Size | Note |
|---|---|---|---|
| 7339 |
Pfu Protease S
|
500 U |
Description
Pfu Protease S is a thermostable endo-type Serine protease with broad specificity toward native and denatured proteins.
Storage
4°C
Source
Bacillus species carrying a plasmid that encodes the Pyrococcus furiosus protease gene.
Form
25 mM Tris-HCl buffer, pH7.6 containing 25% ethanol
Volume
500 units/vial
Purity
Homogeneous in SDS-PAGE
Properties
| Molecular weight: | 42,906 (amino acid composition) 45,000 (SDS-PAGE) |
| Optimum temperature: | 85-95°C |
| Optimum pH: | 6-8 |
| Thermal stability: | retains 80% activity after 3hrs at 95°C, pH7.0 |
| Tolerance to denaturants: | 1% SDS; retains 50% activity after 24 hrs at 95°C, pH7.0 6.4 M urea; retains 70% activity after 1 hr at 95°C, pH7.0 50% acetonitrile; retains 90% activity after 1 hr at 95°C, pH7.0 |
| Inhibitor: | PMSF |
Definition of Activity
One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Suc-AAPF-pNA at 95°C, pH7.0, in one minute.
Suc-AAPF-pNA: N-succinyl Ala-Ala-Pro-Phe p-nitroanilide.
Figure 1 Optimum temperature of Pfu Protease S (pH7.0) Substrate: Suc-AAPF-pNA, Casein
Figure 2 SDS-resistance of Pfu Protease S (95°C, pH7.0)
Note
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