Takara applies a system in which enzyme activities measured in the optimal universal buffers (one of 5 buffers:) are displayed. The relative activity in other universal buffers is expressed as 100% of the optimal universal buffers.
( ) indicates buffers which are likely to be affected by star activity etc. In order to avoid these effects, use of or buffers is recommended. For 15 enzymes (Acc III, Bal I, Bcn I, Bgl I, Bpu1102 I, Cfr10 I, Eam1105 I, Eco52 I, Nru I, PshB I, SnaB I, Ssp I, Taq I or VpaK 11 BI,basal buffer specializedfor each enzyme is supplied).
The compositions of basal buffers vary depending on enzymes. For reference to double digestion, recomendable enzymes for each buffer (relative activity for each reaction mixture and enzyme) are listed.

Relative activity for each reaction buffer

: supplied, activity measured buffer
: recommended buffer

Restriction enzyme	Relative activities (%)
	L	M	H	K	T+BSA	Basal***
Aat II	<20	<20	<20	<20	100	120
Acc I	20	100	<20	(<20)	160	80
Acc II	(260)	100	<20	20	200	160
Acc III	(<20)	(<20)	20	(80)	(<20)	100
Afa I	60	60	40	60	100	100
Afl II	20	80*	<20	<20	140	120
Alu I	100	100	<20	40	200	120
Aor13H I	<20	20	<20	80*	80	100
Aor51H I	80	100	<20	20	120	120
Apa I	100	<20	<20	<20	<20	120
ApaL I	100	20	<20	<20	120	120
Ava I	(<20)	100	20	40	100	120
Ava II	80	100	<20	20	100	100
Bal I	20	20	<20	<20	40	100
BamH I	(<20)	<20	40	100	(<20)	80
Ban II	(120)	(120)	100	80	(100)	100
Bcn I	<20	20	40	60	60	100
Bgl I	<20	<20	20	40	<20	100
Bgl II	<20	20	100	(100)	(60)	100
Bln I	<20	20	40	100	20	120
BmeT110 I	<20	<20	20	100	<20	140
BmgT120 I	<20	<20	100	40	<20	240
Bpu1102 I	<20	<20	<20	40	60	100
BspT104 I	100	60	<20	<20	100	120
BspT107 I	<20	20	80	100	20	100
Bsp1286 I	100	20	<20	<20	60	100
Bsp1407 I	20	60	20	20	100	100
BssH II	100	100	60	20	140	100
BstP I	(<20)	(60)	100	(100)	(100)	100
BstX I	<20	40	100	<20	<20	120
Bst1107 I	(<20)	60	100	100	40	100
Cfr10 I	(<20)	(<20)	(<20)	40	(20)	100
Cla I	40	100	120	100	60	100
Cpo I	<20	<20	80	100	<20	100
Dra I	100	100	60	100	80	80
Eae I	60	100	<20	<20	120	160
Eam1105 I	(<20)	(40)	20	40	(40)	100
EcoO65 I	(20)	(60)	60*	40	40	100
EcoO109 I	100	60	<20	<20	100	160
EcoR I	(20)	(100)	100	(120)	(80)	120
EcoR V	(<20)	(40)	100	(120)	(40)	100
EcoT14 I	(<20)	(40)	100	120	(60)	100
EcoT22 I	<20	20	100	(140)	(20)	120
Eco52 I	<20	<20	<20	<20	<20	100
Eco81 I	<20	100	<20	<20	100	160
Fba I	(<20)	(<20)	(80)	100	(20)	100
Fok I	(20)	(60)	<20	<20	(200)	100
Hae II	80	100	<20	80	140	100
Hae III	60	100	100	60	100	100
Hap II	100	60	<20	<20	100	80
Hha I	80	100	100	120	120	100
Hinc II	20	100	20	40	100	80
Hind III	(60)	100	<20	200	(100)	80
Hinf I	80	100	100	160	60	100
Hin1 I	40	80*	<20	20	60	160
Hpa I	<20	(40)	20	100	(80)	100
Kpn I	100	60	<20	<20	(100)	80
Mbo I	20	40	60	100	40	100
Mbo II	100	60	<20	<20	60	100
Mfl I	100	80	<20	<20	80	100
Mlu I	60	60	100	(100)	60	100
Msp I	80	80	<20	100	100	80
Mun I	(200)	100*	<20	<20	160	100
Mva I	(<20)	(40)	80	100	(20)	120
Nae I	100	<20	<20	<20	100	120
Nco I	(40)	(60)	20	60*	(60)	160
Nde I	<20	40	100	100	80	100
Nhe I	(120)	100	<20	<20	(160)	100
Not I	(<20)	(<20)	20**	<20	(<20)	100
Nru I	0	<20	20	20	<20	100
Nsb I	40	20	<20	60	100	100
PmaC I	100	80	<20	<20	100	100
PshA I	20	40	<20	100	60	160
PshB I	(20)	(40)	20	40	40	100
Psp1406 I	20	60	<20	<20	100	100
Pst I	(<20)	(60)	100	80	(20)	80
Pvu I	(<20)	(20)	(40)	80*	(40)	120
Pvu II	(80)	100	40	<20	(40)	100
Sac I	100	60	<20	<20	80	80
Sac II	40	20	<20	<20	100	40
Sal I	<20	<20	100	(20)	<20	120
Sau 3A I	(60)	80	100	<20	(80)	100
Sca I	(<20)	(<20)	100	(60)	(<20)	100
Sfi I	(40)	100	<20	<20	100	100
Sma I	<20	<20	<20	<20	100	100
Smi I	<10	<20	100	40	<10	100
SnaB I	(20)	(40)	<20	<20	(40)	100
Spe I	(80)	100	80	100	(80)	100
Sph I	(20)	(40)	100	120	(20)	100
Sse8387 I	(120)	60*	<20	<20	(60)	100
Ssp I	(<20)	(60)	40	(100)	(80)	100
Stu I	60	100	60	80	140	100
Taq I	40	80	60	60	80	100
Tth111 I	(20)	80	40	100	(80)	120
Van91 I	<20	(20)	60	100	(60)	100
VpaK11B I	<20	<20	60	(40)	<20	100
Xba I	<20	80*	20	<20	120	120
Xho I	<20	60	100	160	60	100
Xsp I	<20	60	<20	100	160	100

*+0.01% BSA→100% Afl II, EcoO65 I, Fok I, Hin1 I, Mun I, Nco I, Pvu I, Spl I, Sse8387 I, Xba I
**+0.01% BSA+0.01% Triton X-100→100% Not I
*** The composition of basal buffers varies depending on enzymes.

Enzymes classified regarding to universal buffer

L	M	H	K	T+BSA
Afa I	Acc I	Ban II	Afa I	Aat II
Alu I	Acc II	Bgl II	Aor13H I*	Acc I
Aor51H I	Afa I	BmgT120 I	BamH I	Acc II
Apa I	Afl II*	BspT107 I	Ban II	Afa I
ApaL I	Alu I	BssH II	Bcn I	Afl II
Ava II	Aor51H I	BstP I	Bln I	Alu I
BspT104 I	Ava I	BstX I	BmeT110 I	Aor13H I
Bsp1286 I	Ava II	Bst1107 I	BspT107 I	Aor51H I
BssH II	BspT104 I	Cla I	Bst1107 I	ApaL I
	Bsp1407 I	Cpo I		Ava I
Dra I	BssH II	Dra I	Cla I	Ava II
Eae I	Bst1107 I	EcoO65 I*	Cpo I
EcoO109 I		EcoR I	Dra I	Bcn I
Hae II	Cla I	EcoR V	EcoT14 I	Bpu1102 I
Hae III	Dra I	EcoT14 I	Fba I	BspT104 I
Hap II	Eae I	EcoT22 I	Hae II	Bsp1286 I
Hha I	EcoO109 I	Hae III	Hae III	Bsp1407 I
Hinf I	Eco81 I	Hha I	Hha I	BssH II
Kpn I	Fok I*	Hinf I	Hind III
Mbo II		Mbo I	Hinf I	Cla I
Mfl I	Hae II	Mlu I	Hpa I	Dra I
Mlu I	Hae III	Mva I	Mbo I	Eae I
Msp I	Hap II	Nde I	Msp I	EcoO109 I
Nae I	Hha I	Not I**	Mva I	Eco81 I
PmaC I	Hinc II	Pst I	Nco I*
Sac I	Hind III	Sal I	Nde I	Hae II
			Nsb I
Stu I	Hinf I	Sau3A I	Psh AI	Hae III
	Hin1 I*	Sca I	Pst I	Hap II
	Kpn I	Smi I	Pvu I*	Hha I
	Mbo II	Spe I	Spe I	Hinc II
	Mfl I	Sph I	Sph I	Hinf I
	Mlu I	Stu I	Stu I	Hin1 I
	Msp I	Taq I	Taq I	Mbo II
	Mun I*	Van91 I	Tth111 I	Mfl I
	Nhe I	VpaK11B I	Van91 I	Mlu I
	PmaC I	Xho I	Xho I	Msp I
	Psp1406 I		Xsp I	Mun I
	Pvu II			Nae I
	Sac I			Nde I
				Nsb I
	Sau3A I			PmaC I
	Sfi I			Psp1406 I
	Spe I			PshA I
				Psp1406 I
	Sse8387 I*			Sac I
	Stu I			Sac II
	Taq I			Sfi I
	Tth111 I			Sma I
	Xba I*			Stu I
	Xho I			Taq I
	Xsp I			Xba I
				Xho I
				Xsp I

^*+0.01% BSA
^**+0.01% BSA+0.01% Triton X-100

10× Loading Buffer

All Takara's restriction enzymes are supplied with 10× Loading Buffer(1 ml).

Composition

1%		SDS
50%		Glycerol
0.05%		Bromophenol Blue

Compositions of universal buffer

1.	10× L	100 mM	Tris-HCl (pH7.5)
		100 mM	MgCl2
		10 mM	Dithiothreitol
2.	10× M	100 mM	Tris-HCl (pH7.5)
		100 mM	MgCl2
		10 mM	Dithiothreitol
		500 mM	NaCl
3.	10× H	500 mM	Tris-HCl (pH7.5)
		100 mM	MgCl2
		10 mM	Dithiothreitol
		1,000 mM	NaCl
4.	10× K	200 mM	Tris-HCl (pH8.5)
		100 mM	MgCl2
		10 mM	Dithiothreitol
		1,000 mM	KCl
5.	10× T (BSA-free)	330 mM	Tris-acetate (pH7.9)
		100 mM	Mg-acetate
		5 mM	Dithiothreitol
		660 mM	K-acetate
6.	0.1% BSA solved in sterilized water
7.	0.1% Triton X-100

Instructions for use

Universal Buffers are 10× concentration. Please add 1/10 volume of the reaction mixture.
Since 10× T buffer does not contain BSA, be sure to add BSA to a final concentration of 0.01%.
Some restriction enzymes are supposed to exhibit a 100% activity when BSA or Triton X-100 is added to the reaction system. Since BSA or Triton X-100 supplied with enzymes is 10× concentration (0.1%), be sure to add 1/10 volume of the reaction mixture like buffer before starting the reaction.

Instructions for use

Add > 1/10 volume of 10× Loading Buffer to stop enzyme reaction and apply on agarose gel electrophoresis. SDS may precipitate during the storage at room temperature. In case precipitates generate, dissolve in warm bath before use.