Description

This DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. This DNA may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, expression of the cloned DNA can be performed by using of lac promoter. M13 primer is available for DNA sequencing.
Using Deletion Kit for Kilo-Sequencing (Cat.# 6030), DNA sequencing of the cloned DNA with kilo-bases can also be performed.
Those are pUC type of cloning vector plasmid, pHSG298 and pHSG299 possessing kanamycin resistancy as selective markers, while pHSG396 and pHSG398 possessing chloramphenicol resistancy, respectively. pHSG298 and pHSG398 contain the consensus multi-cloning sites as same as pUC18, while pHSG299 contains the consensus one as the same as pUC19 (even though Hind III and Sma I sites in pHSG298 and pHSG299 are not available). Multi-cloning sites of pHSG396 is partially converted sites of pHSG398.

Storage

-20°C

Concentration

250-1,000 µg/ml

GenBank

	Entry Name	Accession No.
pUC18	SYNPUC18CV	L09136
pUC19	SYNPUC19CV	L09137
pHSG299	SYNHSG299	M19415
(pHSG399	SYNHSG399	M19087)*

*Exept the sequence of multi-cloning site, pHSG369 and pHSG398 have the same sequence as pHSG399.

Chain length

pUC18/pUC19	2,686 bp
pHSG298, pHSG299	2,676 pb
pHSG396	2,238 bp
pHSG398	2,227 bp

Purity

• Double-strand covalently closed circular form I (RF I) comprises more than 70%.
• It is confirmed that the multi-cloning site is well-maintained upon sequence analysis by dideoxy method.

Intended usage

• Cloning of a target gene and its expression using lac promoter
• DNA sequencing by using M13 primers
• Determination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat.# 6030)

Form

10 mM Tris-HCl (pH8.0), 1 mM EDTA

Plasmid vector map of pHSG396/398