Description

pTWV228/229 DNA are plasmid vectors which is reconstructed from a replication start of pBR322, ampicillin resistant gene of pUC118/119, IG (intergenic region of M13 phage DNA) and β-galactosidase gene including multicloning site (but Acc I site is not available). Since this vector has less copy number compared with pUC type vector, it is useful to apply for cloning genes that much expression will be harmful to host cells. Moreover, since this vector contains IG of M13 phage DNA, it may allow to recover single-strand DNA instead of double-strand DNA upon infection of helper phage M13KO7.

Storage

-20°C

Concentration

250-1,000 µg/ml

Chain length

4,039 bp

DNA Sequences of each Vector

pTWV228 DNA
pTWV229 DNA

Purity

• Contains over 70% double-strand covalently closed circular form I (RF I).
• It is confirmed that the multi-cloning site is well-maintained upon sequence analysis by dideoxy method.
• It is confirmed that the vector contains a single cleavage site for EcoR I, Sac I, Kpn I, Sma I, BamH I, Xba I, Sal I, Pst I, Sph I and Hind III upon restriction analysis by the enzymes.

Intended usage

• Cloning of target gene
• DNA sequencing by using M13 primers
• Determination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat.#6030)
• Gene expression using lac promoter

Form

10 mM Tris-HCl (pH8.0), 1 mM EDTA

Plasmid vector map of pTWV228/229


The sequence of multi-cloning site of pTWV228/229 is the same as pUC18/19.