HECD-1/SHE78-7/Ncad 1-1-3
for paraffin-embedded tissue
ECCD-1
for inhibition assay
Polyclonal Anti-N-cadherin/Anti-P, E, N-cadherin
for paraffin-embedded tissue

Description

Cadherins are transmembrane glycoproteins of 723-747 amino acids which function as Ca²⁺-dependent adhesion receptors, and are responsible for tight intercellular connections¹⁾. The cadherin family has a number of members including E- (epithelial), P- (placental), and N- (neural) cadherin, which share a common basic structure (see below) but show distinct tissue distributions. The specific binding properties of each member appear to control selective cell-to-cell adhesion. Regulated expression of the members during development implicates involvement in morphogenesis. Recent findings also suggest relationships with tumor invasion and metastasis^{1, 2)}.

Storage

4°C
The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year, or at 4°C for 6 months. Avoid repeated freeze -thaw cycles.

Structure of cadherin

The amino-terminal extracellular portion of the molecule contains sequences that determine the specificity of each cadherin. Calcium is required for cadherinmediated adhesion and also protects cadherins against proteolysis. Clone ECCD-1, PCD-1, and NCD-2 recognize residues around Glu 16 of murine E-cadherin, Gly(31) of murine P-cadherin, and Ser(31) of chick N-cadherin, respectively³⁾.

MAb clones originate from relevant hybridoma cells constructed by fusion between splenocytes of immunized hosts and P3-X63-Ag8 myelomas. HECD-1, SHE78-7, NCC-CAD-299 and Ncad 1-1-3 are purified from ascitic fluids. The other clones are purified from serum-free culture supernatant (see Appendix for applications).

Source

Clone	Cat.#	Immunogen	Immunized host	Subclass	Antibody titer
E-cadherin
HECD-1	M106	Human breast tumor cell line MCF-7	BALB/c mouse	mouse IgG1	1:10,000
SHE78-7	M126	Soluble human placenta E-cadherin	BALB/c mouse	mouse IgG2a	1:10,000
ECCD-1	M107	Mouse teratocarcinoma cell line F9	Wister rat	rat IgG2b
ECCD-2	M108	Mouse teratocarcinoma and mouse liver E-cadherin fragment	Donryu rat	rat IgG2a
P-cadherin
NCC-CAD-299	M127	Human epidermal carcinoma cell line A-431	BALB/c mouse	mouse IgG1	1:4,000
PCD-1	M109	Mouse endoderm cell line PSA5-E	Donryu rat	rat IgG2a
N-cadherin
Ncad 1-1-3	M180	Recombinant human intracellular N-cadherin region (737-876)	BALB/c mouse	mouse IgG1
NCD-2	M110	Chick embryonic neural retina	Wister rat	rat IgG2a
Polyclonal	M142	peptide of human N-cadherin(808-827)-KLH conjugate	rabbit
P,E,N-cadherin
Polyclonal	M143	inter cellular C-terminal peptide of human N-cadherin-KLH conjugate	rabbit

Form

Lyophilized

Monoclonal	(M106, M126, M107, M108, M109, M127, M180, M110) Dissolve in 50 µl of distilled water (final conc.: 2.0 mg/ml, not containing any preservative)
Polyclonal	(M143) Dissolve in 200 µl of distilled water (final conc.: 2.0 mg/ml, containing 0.1% sodium azide)
Polyclonal	(M142)

Supplied Reconstitution Solution (Cat. #M142)

0.1% NaN₃ (in water)

Specificity

Clone	Cat.#	React with	Not react with
E-cadherin
HECD-1	M106	human, guinea pig	bovine, dog, rat, chicken
SHE78-7	M126	human	rat
ECCD-1	M107	mouse	human, bovine, chicken, dog
ECCD-2	M108	mouse, human, dog	bovine, chicken
P-cadherin
NCC-CAD-299	M127	human
PCD-1	M109	mouse	human, chicken
N-cadherin
Ncad 1-1-3	M180	human, rabbit	human E, P-cadherin
NCD-2	M110	chicken, xenopus	human, mouse
Polyclonal	M142	human, bovine, mouse, chicken	human E, P-cadherin
P,E,N-cadherin
Polyclonal	M143	human P, E, N-cadherin chicken N-cadherin, mouse E, P-cadherin

Application

Clone	Cat.#
E-cadherin
HECD-1	M106	Histology on paraffin embedded tissue and frozen tissue sections Western blotting analysis under reducing or non-reducing condition Inhibition assay of E-cadherin dependent cell-cell contact (100 µg/ml)
SHE78-7	M126	Histology on paraffin embedded tissue and frozen tissue sections Western blotting analysis under reducing or non-reducing condition Inhibition assay of E-cadherin dependent cell-cell contact (1 µg/ml)
ECCD-1	M107	Inhibition assay of E-cadherin dependent cell-cell contact (200 µg/ml)
ECCD-2	M108	Histology on frozen tissue sections Western blotting analysis under reducing or non-reducing condition
P-cadherin
NCC-CAD-299	M127	Histology on frozen tissue sections Western blotting analysis under non-reducing and non-heating condition Inhibition assay of P-cadherin dependent cell-cell contact (100 µg/ml)
PCD-1	M109	Histology on frozen tissue sections Western blotting analysis under reducing or non-reducing condition Inhibition assay of P-cadherin dependent cell-cell contact (60 µg/ml)
N-cadherin
Ncad 1-1-3	M180	Histology on paraffin embedded tissue and frozen tissue sections Western blotting analysis under non-reducing and non-heating condition Immunoprecipitation
NCD-2	M110	Histology on frozen tissue sections Western blotting analysis under reducing or non-reducing condition Inhibition assay of N-cadherin dependent cell-cell contact (100 µg/ml)
Polyclonal	M142	Histology on paraffin embedded tissue and frozen tissue sections Western blotting analysis under non-reducing and non-heating condition
P,E,N-cadherin
Polyclonal	M143	Histology on paraffin embedded tissue and frozen tissue sections Western blotting analysis under reducing or non-reducing condition

Tissue distribution of cadherins (includes transient expression)

	Early embryo (until neurulation)	Late embryo (post-neurulation)
E-cadherin9) (M.W. 124,000)	· All cells at preimplantation · Embryonic ectoderm · Extraembryonic ectoderm · Endoderm	· Most epithelial tissues · Sensory ganglion12) · Cephalic neuroepithelium13)
P-cadherin7, 9) (M.W. 118,000)	· Extraembryonic ectoderm · Notochord · Lateral plate mesoderm · Visceral endoderm	· Placenta · Mesothelium · Pigmented retina · Basal layer of epidermis and other squamous epithelia
N-cadherin9, 10) (M.W. 127,000)	· Mesoderm · Notochord	· Neural tissues · Lens8) · Cardiac and skeletal muscles · Nephric primordia · Mesothelium · Primordial germ cells

Not all of the tissues are listed (for details, see references and citations therein).

Disruption of tumor cell-cell adhesion by anti-E-cadherin MAbs

Phase-contrast microscopic observation of A-431 cells (x 200) after they were incubated in 10% FCS-supplemented medium containing HECD-1 or SHE78-7 MAbs (50 µg/ml at the final concentration) for 30 minutes.