Description

pSTV28/29 DNA are plasmid vectors which is reconstructed from a replication start of pACYC184, chloramphenicol resistant gene of Tn9 and β-galactosidase gene including pUC18/19 multi-cloning site (but Xba I, Acc I site are not available).
Since this vector has less copy number compared with pUC type vectors, it is useful to apply for cloning that much expression will be harmful to host cells. Moreover, since this vector contains a replication start of pACYC184, it is allowed co-presence into the same cell with plasmid vector like pUC, pBR and so on.

Storage

-20°C

Concentration

250-1,000 µg/ml

Chain length

2,999 bp

DNA Sequences of each Vector

pSTV28 DNA
pSTV29 DNA

Purity

• Contains over 70% double-strand covalently closed circular form I (RF I).
• It is confirmed that the multi-cloning site is well-maintained upon sequence analysis by dideoxy method.
• It is confirmed that the vector contains a single cleavage site for EcoR I, Sac I, Kpn I, Sma I, BamH I, Sal I, Pst I, Sph I and Hind III upon restriction analysis by the enzymes.

Intended usage

• Cloning of target gene
• DNA sequencing by using M13 primers
• Determination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat.#6030)
• Gene expression using lac promoter

Form

10 mM Tris-HCl (pH8.0), 1 mM EDTA

Plasmid vector map of pSTV28/29