Description

pAUR123 is an S.cerevisiae vector for protein expression derived from pAUR112. It contains a constitutive promoter of ADH1 gene (Alcohol dehydrogenase 1 gene) for protein expression in yeast cells. (Note: This vector is not suitable for high expression of protein.)

Storage

–20°C

Concentration

0.5-1.0 µg/µl

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Chain length

6,982 bp

Purity

• Contains over 70% double-stranded covalently closed circular form I (RF I) DNA
• Shown to retain cloning site by dideoxy sequencing method
• Shown to be cleaved at a single site by restriction enzymes specific to these sites

GenBank

Accession No.: AB012284

Intended usage

This is a vector for transformation of yeast S. cerevisiae using antibiotic Aureobasidin A (shuttle vector for protein expression). It is maintained as a plasmid in yeast cells.