Protein Expression Shuttle Vector for S. Pombe
pAUR224 DNA
| Cat.# | Product | Size | Note |
|---|---|---|---|
| 3603 |
pAUR224 DNA
|
20 µg |
Description
pAUR224 vector has a mutant aur1R gene (AbA-resistant gene) derived from Schizosaccharomyces pombe as a selective marker. pAUR224 is an E. coli-yeast shuttle vector for protein expression and contains CMV (cytomegalovirus) promoter which express in S. pombe constitutively.
Storage
–20°C
Concentration
0.5-1.0 µg/µl
Form
10 mM Tris-HCl (pH8.0), 1 mM EDTA
Chain length
7,638 bp
Purity
-
Contains over 60% double-stranded covalently closed circular form I (RF I) DNA
Shown to retain cloning site by dideoxy sequencing method
Shown to be cleaved at a single site by restriction enzymes specific to these sites
Intended usage
This is a vector for transformation of yeast S. pombe using antibiotic Aureobasidin A (shuttle vector for protein expression).
aur1R
AbA-resistant gene in S. pombe
ars1
Replication origin in S. pombe
PCMV
Promoter of CMV
SV40polyA
PolyA signal from SV40
AmpR
Selective marker in E. coli
ori
Replication origin in E. coli
IG
Intergenic region of M13 phage
Cloning sites of pAUR224 DNA
Note
- All products are intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc
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