Description

pAUR316 is a shuttle vector including AMA1, which can replicate autonomously in Aspergillus nidulans. This vector contains ampicillin resistant gene (Amp^R) as selective marker for E. coli and Aureobasidin A-resistant gene (aurA^R) as selective marker for A. nidulans. The restriction endonuclease sites of BamH I, Aor51H I, Afl II, Bgl II and Sse8387 I can be used as a cloning site.

Storage

-20°C

Concentration

0.5-1.0 µg/µl

Form

10 mM Tris-HCI (pH8.0), 1 mM EDTA

Chain length

11,663 bp

Purity

• Contains over 60% double-stranded covalently closed circular form I (RF I) DNA.
• Shown to retain cloning site by dideoxy sequence method.
• Shown to be cleaved at a single site by restriction enzymes specific to these sites.

Intended usage

This is a vector for transformation of A. nidulans using antibiotic Aureobasidin A. It is maintained as a plasmid in fungi.

Restriction enzyme map of pAUR316 DNA



Cloning sites of pAUR316 DNA