Description

pPTR I is a chromosomal integrating and E. coli-Aspergillus shuttle vector. In Aspergillus cells, pPTR I does not replicate autonomously and is maintained only when integrated into chromosome. This vector has an ampicillin resistant gene, Amp^R, as the selection marker for E. coli and a Pyrithiamine resistant gene, ptrA, as that for Aspergillus. Since it has cloning sites, containing Hind III, Pst I, Sma I and Kpn I, on lacZ region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal.

Manufactured by HAKUTSURU SAKE BREWING CO., LTD.

Storage

-20°C

Concentration

1 µg/µl

Form

10 mM Tris-HCI (pH8.0), 1 mM EDTA

Chain length

4,782 bp

Purity

• Contains over 70% double-stranded covalently closed circular form I (RF I) DNA.
• Shown to retain cloning site by dideoxy sequencing method.
• Shown to be cleaved at a single site by restriction enzymes specific to these sites.

Intended usage

This is a vector for transformation of Aspergillus species using Pyrithiamine which is metabolic competitive analog of thiamine.

Note

As the presence of thiamine causes the remarkable decrement of selection efficiency by Pyrithiamine, the media and the reagents without thiamine should be used for this transformation system.

Restriction enzyme map of pPTR I DNA



Cloning sites of pPTR I DNA