Autonomously Replicating Vector for S. cerevisiae
pAUR112 DNA
| Cat.# | Product | Size | Note |
|---|---|---|---|
| 3601 |
pAUR112 DNA
|
20 µg |
Description
pAUR112 is a vector including CEN/ARS which can replicates autonomously in yeast cells, therefore is stable in cell division. The vector also contains mutant AUR1-C gene derived from S. cerevisiae that confers Aureobasidin A- resistance on yeast cells.
Storage
–20°C
Concentration
0.5-1.0 µg/µl
Form
10 mM Tris-HCI (pH8.0), 1 mM EDTA
Chain length
7,104 bp
Purity
-
Contains over 70% double-stranded covalently closed circular form I (RF I).
Shown to retain cloning site by dideoxy sequencing method.
Shown to be cleaved at a single site by restriction enzymes specific to these sites.
GenBank
Accession No.: AB012283
Intended usage
This vector is used for transformation of the yeast Saccharomyces cerevisiae using the antibiotic Aureobasidin A. It is maintained as a plasmid in yeast cells.
Target gene and amplified size
| Primer Set | Target gene | Amplified size |
| SIN-1&2 (Cat.# S018) | invA gene of Salmonella sp. | 378 bp |
| STN-1&2 (Cat.# S019) | Enterotoxin gene of Salmonella sp. | 264 bp |
Cloning sites of pAUR112 DNA
Note
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