Description

pAUR101 is a chromosomal integrating vector for Saccharomyces cerevisiae. This vector does not replicate autonomously in yeast, and is maintained in yeast cells only when integrated into chromosome by recombination. This vector has a novel drug-resistant selective marker, AUR1-C gene, which is a mutant gene derived from S. cerevisiae and confers Aureobasidin A-resistance on yeast cells. The vector linearized by cutting at a single restriction enzyme site (BstP I, EcoO65 I, BsiW I or Stu I) in the ORF of AUR1-C gene is efficiently integrated in aur1 gane region on S. cerevisiae chromosome.

Storage

–20°C

Concentration

0.5-1.0 µg/µl

Form

10 mM Tris-HCI (pH8.0), 1 mM EDTA

Chain length

6,687 bp

Purity

• Contains over 70% double-stranded covalently closed circular form I (RF I).
• Shown to retain cloning site by dideoxy sequencing method.
• Shown to be cleaved at a single site by restriction enzymes specific to these sites.

GenBank

Accession No.: AB012282

Intended usage

This vector is used for transformation of the yeast S. cerevisiae using the antibiotic Aureobasidin A (Chromosomal integrating shuttle vector). This vector is also applicable for transformation of Kluyveromyces marxianus, K. lactis and Candida glabrata.

Restriction enzyme map of pAUR101 DNA


Cloning site of pAUR101 DNA


Unique site for linearization of pAUR101 DNA