Description

This product is a plasmid vector for producing a retrovirus vector with high expression efficiency.
By transfecting the vector into an appropriate packaging cell line, the vector expresses transient or stable transcribed product containing virus packaging signal(Ψ), target gene and selective marker.
This vector possesses LTR and Ψ (viral packaging signal), but not the structural gene necessary for particle formation and replication, which are gag, pol, and env. Because this vector contains intron with high ability of splicing, it yields highly transcription efficiency. pMEI-5 Neo DNA (Cat.# 3655) contains neomycin resistance gene as a selective marker.
2-8 fold higher gene expression level can be expected compared to pDON-AI-2 series.

Storage

-20°C

Form

10 mM Tris-HCl (pH8.0), 1 mM EDTA

Chain length

pMEI-5 Neo DNA	: 5,820 bp
pMEI-5 DNA	: 4,689 bp

Preparation

cccDNA was purified by CsCl-EtBr ultracentrifugation.

Purity

1. Contains over 70% double-stranded covalently closed circular DNA(RFI) by agarose gel electrophoresis.
2. Confirmed to maintain cloning sites by dideoxy sequencing method.
3. Confirmed to be cleaved at a single site by restriction enzymes PmaC I, Apa I, Sac II,Not I, Bgl II, Cla I, BamH I, Sal I, Hpa I and Xho I.
(Xho I is pMEI-5 only)

Application

Retrovirus-mediated gene transfer into mammalian cells.