Description

A real-time PCR that offers an accurate and reproducible quantification is indispensable for gene expression analysis.
Takara Bio launched a real-time PCR instrument for 96 plate. Thermal Cycler Dice™ Real Time is a compact system for 96 plate type with high-performance hardware and easy-to-use software. We recommend this system as a "Perfect" real-time PCR instrument with confidence.

This product is not available in the U.S. and Europe.

Features

- High-performance hardware -

♦ Temperature control and Optical system
This system uses the same Peltier element that is used in the Thermal Cycler Dice series to minimize the temperature difference between wells. And this system has reliable detection system using the camera shoot method to detect fluorescence. As a result, this system realize a high uniformity and reproducibility.

• Highly reliable results
  The high uniform results is obtained in all 96 wells.
  The same reactions were performed in all 96 wells and Ct values (Threshold Cycle) were compared. The result shows the excellent uniformity of each well.
  

  Template: λDNA 500 fg Reagent: SYBR® Premix Ex Taq™ (Perfect Real Time) (Cat.# RR041A/B)

  Precision Average of Ct = 23.6 Standard deviation = 0.0931 CV(%) = 0.39

  
  



• 2-fold difference can be analyzed
  To perform an accurate real-time PCR analysis, a high performance is needed for both the instrument and reagents. You can analyze the 2-fold difference of template concentration using SYBR^® Premix Ex Taq™ (Perfect Real Time) (Cat.# RR041A/B) with this system.
  
  

  Template:	cDNA synthesized from mouse total RNA 6 orders, 1:2 serial dilution (312.5 pg to 10 ng) * The cDNA amounts correspond to the total RNA amount. N=3
  Target:	Gapd gene

- Easy-to-use software -

♦ Software that is fun to use
The user interface of this software is simple and intuitive, consisting of three main screens. It is easy-to-use for beginners and satisfactory for advanced users.

• Plate Setup
  Enter the sample information on this screen. "Plate Document Editor" is a set of useful tools to enter the information and helps your plate setup. It is also possible to import the setting from another existing file.
• Thermal Profile Setup
  Define the PCR conditions and select the filters on this screen. The setup can be done easily using the pull-down menu. And the thermal profile is shown visually. Detailed settings of the PCR condition are possible by using the extended menu.
• Result / Analysis
  Adjust the analysis settings and view results on this screen. The results are shown on two screens, each of which can be used for any kind of graph. You can verify the data from various view points.
  

  Plate Setup	Thermal Profile Setup	Result / Analysis screen

♦ Various analytical methods
Various analytical methods can be switched by just one click.

• Calculate the Ct (Threshold Cycle) value
  Crossing Point Method
  2nd Derivative Maximum Method
• Relative quantification analysis
  Standard Curve Method
  ΔΔCt (Comparative Ct) Method

♦ Two optical channels and More
2 type of filters, FAM™/SYBR^® Green I and ROX™/Texas Red^®, are installed in the instrument. If necessary, you can add two more filters (HEX/VIC^®, Cy™5) to those as a option.
This system does not need ROX™ reference dye to normalize the detection error. It enables to use the ROX™ filter for detection and also to perform multiplex PCR with FAM™ & ROX™ filters. It is possible to perform not only SYBR^® Green I assay but also various kinds of probe assays, such as TaqMan^®, Molecular Beacon and Cycling probe.

Filter	Excitation (nm)	Emission (nm)
FAM™ (SYBR® Green I)	482	536
ROX™ (Texas Red®)	562	624

♦ Compact design
The compact size of this system is achieved by the slide-lid with built-in mirror and the halogen lamp that is set in transverse. The operation is easy even if it is set on a shelf because the moving part of it is only the slide-lid. The sound under operation is quiet. All these features make it an ideal instrument for every lab.

Specification

Description	Thermal Cycler Dice™ Real Time System
Cat.#	TP800
Dimensions	290(W)×517(D)×397(H)mm
Weight	23.5 kg
Voltage requirements	AC100~240V, 50/60Hz, 620 W
Heating and Cooling system	Peltier element
Excitation source	Halogen lamp100 W
Maximum heating speed	3.0°C/sec.
Maximum cooling speed	2.0°C/sec.
Temperature control range	4.0~99.9°C (every 0.1°C)
Temperature control accuracy	±0.5°C at 94°C/55°C ±2.0°C at 4°C
Filter unit	2 types (FAM™/SYBR® Green I, ROX™/Texas Red®) * 4 types at maximum
Detector	CMOS camera
Dissociation curve analysis	Possible
Real time monitoring	Possible
Sample capacity	96 samples
Reaction volume	Recommended: 25 μl Maximum: 50 μl
Container	0.2 ml tube, 96 well plate
Computer OS	Windows® XP with PC

Specifications are subject to change for modification without notice.

Related Information

• Brochure: Thermal Cycler Dice™ Real Time System