PrimeScript™ RT reagent Kit (Perfect Real Time)
| Cat.# | Product | Size | Note |
|---|---|---|---|
| RR037A | PrimeScript™ RT reagent Kit (Perfect Real Time) | 200 reactions | |
| RR037B(A×4) | PrimeScript™ RT reagent Kit (Perfect Real Time) | 800 reactions |
Description
PrimeScript™ RT reagent Kit is designed to perform the reverse transcription optimized for real-time RT-PCR. It uses PrimeScript™ RTase, which features excellent extendibility and makes fast, efficient cDNA template synthesis for Real Time PCR possible. The experimental procedure is simple and suitable for high through-put analysis. This kit can be used in combination with Real Time PCR reagent, SYBR® Premix Ex Taq™ (Perfect Real Time) or Premix Ex Taq™ (Perfect Real Time) for 2 step Real Time RT-PCR.
The optimized protocol for assay can be selected in each assay condition using either SYBR® Green
or TaqMan® probe.
This product is not available in the U.S.
Storage
-20°C
Kit Components (for 200 reactions)
There is a partial modification from Lot AK1101 as to the Kit Components.
| 1. 5 × PrimeScript™ Buffer (for Real Time)*1 | 400 µl | |
| 2. PrimeScript™ RT Enzyme Mix I | 100 µl | |
| 3. Oligo dT Primer | 50 µM | 100 µl |
| 4. Random 6 mers | 100 µM | 400 µl |
| 5. RNase Free dH2O | 1 ml | |
| 6. EASY Dilution (for Real Time PCR)*2 | 1 ml |
| *1: | contains dNTP Mixture and Mg2+. |
| *2: | It can be used to prepare the dilution series of total RNA or cDNA, which is required for establishing the standard curve. In contrast to dilution with water or TE, EASY Dilution facilities accurate low concentration duilution. Diluted template solution can be applied as the template for reverse transcription or PCR reactions, because Easy Dilution does not inhibit either reverse transcription or PCR enzyme activity. EASY Dilution is also available separately. EASY Dilution (for Real Time PCR)(TaKaRa Cat.#9160) Note: Easy Dilution has been tested with TaKaRa's Bio real-time PCR reagent. Compatibility with products from other manufacturers has not yet been verified. |
Features
| (1) | Makes fast, efficient synthesis of cDNA templates for Real Time PCR possible. This kit is best suited for two step real-time RT-PCR. |
| (2) | The kit includes Random 6 mers and Oligo dT Primer for use as reverse transcription primers. The reaction can be performed using mixture these two primers, or the primer can be selected based on the purpose of the experiment. Futhemore, Gene Specific Primers can be used for specific gene detection. |
| (3) | A standard curve must be generated for the quantitation of Real-Time RT-PCR. Dilution of total RNA or cDNA after reverse transcription is necessary because low concentrations are required for a viable standard curve. However, dilution with water or TE can narrow the range of the curve due to unstable dilution at low concentrations. Using EASY Dilution (for Real Time PCR), for dilution causes the results to be accurate at lower concentrations and facilitates creation of a wide-range standard curve. |
Note
- All products are intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc
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