Glycocalicin EIA Kit Ver.20-52
| Cat.# | Product | Size | Note |
|---|---|---|---|
| MK105 | Glycocalicin EIA Kit Ver.20-52 | 96 assays |
Description
Glycocalicin is a 110-135 kDa proteolytic fragment of the platelet glycoprotein Ib ƒ¿ - chain (GPIb), which presumed to be cleaved by cell surface protease proteolysis, such as calpain1). Glycocalicin is believed to include the binding domain structure of GPIb for ristocetin-dependent von Willebrand factor2,3), thrombin4), and quinidine-dependent antibody receptor5). Recently, it has been shown that Glycocalicin circulated in vivo6) and the further studies has indicated that plasma levels of Glycocalicin might be a useful marker in the classification of thrombocytopenic disorders7,8). And also, it has been shown that Glycocalicin levels elevated in the supernatants of platelet concentrates during storage, which might reflect the shedding of GPIb from stored platelet membranes(9,10).
The Glycocalicin EIA Kit is an in vitro enzyme immunoassay (EIA) kit for quantitative determination of human Glycocalicin in platelet extract, plasma and serum.
Storage
4°C
Kit components
| 1. | Antibody Coated Microtiterplate | 1 plate (8 well × 12 strips) |
| 2. | Antibody - POD Conjugate (PL52 - 4) | 1 vial (for 11 ml) |
| 3. | Standard | 1 vial (640 ng) |
| 4. | Sample Diluent | 2 vials (11 ml × 2) |
| 5. | Substrate Solution (TMBZ) | 1 vial (12 ml) |
Performance
| Range of assay: | 10 - 640 ng/ml |
| Sensitivity: | 10 ng/ml |
| Assay duration: | 2 hours and a half for assay protocol |
| Specificity: | This kit specifically measures human Glycocalicin |
| Test specimen type: | Human pletelet extract, plasma, serum; If each test sample is run in duplicate, about 210 µl (100 µl per assay well plus approx. 10 µl for waste per transfer) is required. |
Note
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