Description

Cryonase™ Cold-active Nuclease is a Recombinant Endonuclease originated from a psychrophile, Shewanella sp., which is purified from E. coli. This enzyme can digest all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) even on ice. Therefore, this enzyme is useful to digest nucleic acids in the samples including heat unstable substances such as proteins.

Components

10,000 U (20 U/µl×500 µl)

Storage

-20°C

Source

Purified from E. coli

Form

10 mM	Tris-HCl, pH7.5
20 mM	NaCl
2 mM	MgCl2
50%	glycerol

Unit definition

One unit is the amount of the enzyme that increases the absorbance at 260 nm by 0.001 per minute at 37°C and pH7.5, with salmon sperm DNA as a substrate.

Purity

• Protease activity is less than detection limit.

Usage

• Degradation of genomic DNA
• Decrease of the viscosity in protein solution extracted from E. coli cells
• Pretreatment of samples for two-dimensional electrophoresis
• Pretreatment for virus purification

This product was developed based on the results of genome analysis of Shewanella sp. Ac 10 strain, which was offered from Dr. Nobuyoshi Ezaki of Kyoto University Institute for Chemical Research and his associates to TaKaRa Bio, through collaboration with them.