Description

PrimeScript™ II 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize 1st strand cDNA from total or poly(A)⁺ RNA using PrimeScript™ II RTase. A main factor interfering cDNA synthesis is based on non-specific binding of reverse transcriptase itself to higher structure of RNA in the case of RNA having complex structure or long-length RNA as a template. In addition, non-specific elongation derived from miss-priming of a reverse transcriptase cause a very harmful influence in RT-PCR or production of full-length cDNA. PrimeScript™ II RTase, which is constructed by further improvement using an accessory protein from PrimeScript™ RTase developed by TaKaRa Bio, is a reverse transcriptase depressing extremely the harmful factors in the reaction of cDNA synthesis. This kit can synthesize full-length cDNA products efficiently and keep excellent highly efficient of polyA usage and synthesis speed at standard temperature (42°C) of reverse transcription in elongation reaction from polyA using oligo dT primer. And inhibition of cDNA synthesis does not occur in case that the reaction mixture stand for long time on ice before the reaction of reverse transcription, because this kit suppress completely to occur non-specific elongation in the preparation of reaction mixtures. 1st strand cDNAs synthesized with this kit can be used for variety of applications including 2nd strand synthesis, hybridization, and PCR amplification. Furthermore, this kit is useful for application to construct high-quality, full-length cDNA.

This product is not available in the U.S.

Components

(for 50 reactions)

Storage

-20°C