Description

This kit is a primer set for real-time PCR to detect GFP (EGFP, AcGFP1) and lacZ, which are widely used for gene marker introduced in transgenic mouse. It is possible to screen of transgenic mouse by detecting these marker genes with real-time PCR.

The primer sets (Ywhaz, Raver2) for detecting genes on mouse genomic DNA are also included as reference. Therefore, it is possible to make a comparison of the transgene contents between samples by relative quantification method.

Components

(for 100 reactions^*1 in each primer)

	Concentration	Amount	Product size
1. GFP_primer-1*2	2 µM each	500 µl	127 bp
2. GFP_primer-2*3	2 µM each	500 µl	162 bp
3. lacZ_primer-1*4	2 µM each	500 µl	96 bp
4. lacZ_primer-2*4	2 µM each	500 µl	141 bp
5. Reference_primer-1*5	2 µM each	500 µl	115 bp
6. Reference_primer-2*6	2 µM each	500 µl	89 bp

*1 Frequency when using SYBR^® Premix Ex Taq™ II with 25 µl reaction volume.
*2 GFP_primer-1 is designed for consensus sequence with EGFP and AcGFP1.
*3 GFP_primer-2 is designed for EGFP. It is not used for AcGFP1 detection.
*4 lacZ_primer-1 and -2 is designed for lacZ (β-galactosidase) gene and positioned at different sites.
*5 Reference_primer-1 is for a part of Ywhaz gene on mouse chromosome 15.
*6 Reference_primer-2 is for a part of Raver2 gene on mouse chromosome 4.

Storage

-20°C

Reagents and instruments not supplied in this kit

• real-time PCR reagent
  SYBR^® Premix Ex Taq™ II (Perfect Real Time) (Cat. #RR081A/B)
  SYBR^® Premix Ex Taq™ (Perfect Real Time) (Cat. #RR041A/B)
• 1.5 ml microtube
• Micropipette and tips
• real-time PCR instrument
  Thermal Cycler Dice™ Real Time System (Cat. #TP800)
    Note : This is not available in the U.S. and Europe.