Description

Fruit-mate™ for RNA Purification (hereafter described as Fruit-mate) is a supportive reagent for RNA extraction by using RNAiso Plus or FastPure™ RNA Kit. This product applies when extracting total RNA from plants sample (root crop, fruit, seed, etc.) that contains large quantities of polysaccharides or polyphenols. The Fruit-mate contains non-ionic polymer and binds to substances such as polysaccharides or polyphenols. These substances can be easily removed by adding this product to the homogenized sample, binding with the product, and performing centrifugation. The RNA extraction from plants which have been difficult only by the RNA extraction kits are performed effectively with the Fruit-mate.

Use in combination with RNAiso Plus, add this product to a homogenized sample and mix well, and then remove the insoluble substance by centrifugation. Add equal amounts of RNAiso Plus to the collected supernatant, then add chloroform and mix well. After separate to 3 layers by centrifugation, collect top liquid layer containing RNA, and recover total RNA by isopropanol precipitation. Thereby, all the processes of total RNA extraction are completed in about 1 hour.
Use in combination with the FastPure™ RNA Kit, add this product to a homogenized sample and mix well, and then remove the insoluble substance by centrifugation. After prepare lysate by adding LB to the collected supernatant, recover total RNA by using the Filter cartridge with polymer membrane for nucleic acid collection. It takes about 30 minutes to complete total RNA extraction process. The total RNA isolated can be used for RT-PCR, northern blot analysis, or mRNA isolation.

[ Notes ]

1. The plants (or its part) which are confirmed with improvement in RNA recovery ratio by using the Fruit-mate are the followings：
   
   < Plants (parts) in which the effects of Fruit-mate are observed >
   Mini tomato (fruit), Banana (flesh), Tomato (seed), Rice (seed), Potato (rhizome), Mandarin orange (peel), Pine (leaf) Aloe (leaf), Mango (fruit), Arabidopsis (seed)
   
   This product is designed for plants that contain high content of polysaccharide or polyphenol. Depending on types of plant (or its part), however, use of this product may result in no effect or in reduction in recovery ratio of total RNA. Be careful when testing plant (parts) except for those listed above.
2. RNAiso Plus is a product for RNA extraction based on AGPC method. This product is not available in the U.S. and Europe.

Storage

Room temperature
* In the case of store under 20°C, precipitate might be appeared. In such case, use it after dissolving the precipitate at 37°C.

Reagents not supplied in this product

For combination with RNAiso Plus

• RNAiso Plus (Cat. #9108/9109) ^*
• Chloroform
• isopropanol
• 75% ethanol
• RNase-free water or TE

* This product is not available in the U.S. and Europe.

For combination with FastPure™ RNA Kit

• FastPure™ RNA Kit (Cat. #9190)
• 2-mercaptoethanol (2-ME)
• ethanol (>99 %)
• DNase I (RNase-free) (Cat. #2215A/B) (only when it is necessary)
• RNase-free water