Dr. GenTLE® (from Yeast) High Recovery
| Cat.# | Product | Size | Note |
|---|---|---|---|
| 9082 | Dr. GenTLE® (from Yeast) High Recovery | 30 reactions |
Description
Dr. GenTLE® (from Yeast) High Recovery is designed to efficiently extract and purify genomic DNA from yeast cells by using both digestion enzyme of cell wall and salting out for DNA purification. Procedure for genomic DNA purification using this kit is as the following; recovery of yeast cells by centrifugation, digestion of yeast cell wall by GenTLE Yeast Solution A (contains cell wall lytic enzyme), cell lysis by GenTLE Yeast Solution B, salting-out for protein removal by adding GenTLE Yeast Solution C and centrifuging, and DNA recovery by isopropanol precipitation.
This kit is useful to isolate genomic DNA, which is available for general genetic engineering, such as Southern blotting, PCR, or restriction enzyme treatment, from various species of yeast listed on the Table A and B in below. For standard experiment, 4 - 10 µg of genomic DNA (35 - 200 kb) can be obtained from 1 - 2×108 yeast cells.
| A | Yeast from which DNA can be prepared | Ashbya, Candida, Debaryomyces, Endomyces, Eremothecium, Hanseniaspora, Hansenula, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pullularia, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schizosaccharomyces, Selenozyma, Trigonopsis, Wickerhamia |
|---|---|---|
| B | Yeast from which DNA can be prepared (Strain-dependent) | Bretanomyces, Cryptococcus, Nadsonia, Pichia, Rodosporidium, Schwanniomyces, Stephnoascus, Torulopsis |
| C | Yeast from which DNA cannot be prepared | Bullera, Pityrosporum, Rhosotorula, Sporidiobolus, Sporobolomyces, Stetigmatomyces, Trichosporon |
Components
(For 30 reactions)
| 1. | GenTLE Yeast Solution A*1 | 15 ml |
| 2. | GenTLE Yeast Solution B *2 | 3 ml |
| 3. | GenTLE Yeast Solution C | 6 ml |
| 4. | TE Buffer | 6 ml |
*1 GenTLE Yeast Solution A contains enzyme. Avoid mixing vigorously or doing operations which may inactivate enzyme.
*2 If precipitates appeared in GenTLE Yeast Solution B, use after dissolving completely the precipitates by heating up at 37°C.
Storage
4°C
Only GenTLE Yeast Solution B can be stored at room temperature. When stored at low temperature, precipitates may appear. If precipitates appear, dissolve precipitates completely by heating at 37°C before use.
Reagents and instruments not supplied in this kit
- Centrifuge
- isopropanol
- 70% ethanol
- tubes
- Thermostat bath (for incubation at 37°C)
- Heat block (for heating at 70°C)
Note
- All products are intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc
- Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC.
- If you require licenses for other use, please call at +81 77 543 7247 or contact from our website at www.takara-bio.com
- Please confirm the content about the license or patent used in this document that relates to the Takara Bio product by clicking the license mark.
Moreover, please confirm the "Limited Use Label License" or "patent" concerning the product of another manufacturers or respective owners in their Web site/catalog etc.
