RNAiso Plus
| Cat.# | Product | Size | Note |
|---|---|---|---|
| 9108 | RNAiso Plus | 100 ml | |
| 9109 | RNAiso Plus | 200 ml |
This product is available in Japan, Korea and China only.
Description
RNAiso Plus is a total RNA extraction reagent which can isolate RNA easily and rapidly from animal or plant tissues and cultured cells. After homogenizing the tissues or cells in the RNAiso Plus solution, add chloroform to the homogenate solution, mixed well, and then centrifuge to separate the solution into three layers. The top layer will be a clear liquid containing RNA, the middle layer will be a semi-solid containing DNA, and the bottom layer will be a red colored organic solvent containing proteins, polysaccharides, fatty acid, cell debris, and small amount of DNA.
Collect the top liquid layer and pipet into a new tube. Be careful not to collect any of the middle layer. Perform an isopropanol precipitation to extract the total RNA.
Using the RNAiso Plus, the total RNA extraction process can be done in about one hour. The isolated total RNA is intact and does not contain a small amount of DNA or proteins, thus it can be used for RT-PCR*, Northern blot analysis, mRNA isolation, and in vitro translation reactions.
* For RT-PCR, even an extremely small amount of genomic DNA could affect the result, so treat with DNase I (RNase-free) (Cat. #2215A/B, #2220A/B) before use.
Components
RNAiso Plus (Cat. #9108)* 100 ml
RNAiso Plus (Cat. #9109)* 200 ml
* Contains a protein denaturator, so avoid skin contact. If comes in contact with eyes or skin, wash with water immediately and seek for medical suggestion by a doctor.
Shipping
room temperature
Storage
4°C
Store in a dark place to retain activity.
Materials required but not provided
- Chloroform
- Isopropanol
- 75% ethanol (prepared with DEPC-treated water)
- RNase-free water
Required Amount of RNAiso Plus for extraction
| Sample types and amounts | RNAiso Plus amount (ml) |
|---|---|
| Adherent cells on 10cm2 petri Dish 5×106 - 1×107 of non-adherent cells 100 µl of white blood cells |
1 - 2 1 2 |
| 50 - 100 mg of tissue sample • Tissue which RNA can be easily extract • Tissue which RNA can be difficult to extract (kidney, spleen, born, and cartilage) |
1 2 |
| 15 - 30 mg of plant material (containing small amount of polysaccharide and phenol) | 1 |
The typical amount of total RNA extracted using RNAiso Plus
| Tissue samples | Sample amount | Amount of total RNA extracted |
|---|---|---|
|
Liver Kidney Skeletal muscle Brain HL-60 cultured cell Tobacco leaf White blood cell Whole blood* |
1 g 1 g 1 g 1 g 1×107 cells 1 g 1×107 cells 1 ml |
About 5000 µg About 3000 µg About 1500 µg About 1500 µg About 100 µg About 1000 µg About 100 µg 15 - 20 µg |
* Used 1 ml of RNAiso Plus for 100 µl of whole blood
Note
- All products are intended to be used for research purpose only. They are not to be used for drug or diagnostic purposes, nor are they intended for human use. They shall not to be used products as food, cosmetics, or utensils, etc
- Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC.
- If you require licenses for other use, please call at +81 77 543 7247 or contact from our website at www.takara-bio.com
- Please confirm the content about the license or patent used in this document that relates to the Takara Bio product by clicking the license mark.
Moreover, please confirm the "Limited Use Label License" or "patent" concerning the product of another manufacturers or respective owners in their Web site/catalog etc.
