Description

pRI 909 DNA is a binary vector which has a region of T-DNA for plant transformation. It is originated from Ri plasmid of Rhizobium (Agrobacterium) rhizogenes, lacking their vir region. pRI 909 DNA is also a shuttle vector, and replicates autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, this vector is a high copy number plasmid because it has a replication origin same as that of pUC type plasmid (ColE1 ori), and it is maintained stably in Rhizobium (Agrobacterium) also with mutant type replication origin of Ri plasmid (Ri-ori). This vector has a kanamycin resistant gene NPTIII, as the selection marker for E. coli and Rhizobium (Agrobacterium), a mutant type kanamycin resistant gene NPTII, as the selection marker for plant, and the same multi-cloning sites as pUC type plasmids are present.
This vector is available for plant transformation in combination with Rhizobium (Agrobacterium) by binary vector method. It is possible to integrate target gene in plant chromosome stably using this vector because its cloning site is located at the position closer to Right Border(RB) of T-DNA than the selection marker (NPT II) for plant, so the target gene is not deleted.

Storage

-20°C

Form

10 mM	Tris-HCl, pH 8.0
1 mM	EDTA

Preparation

Purification by column treatment

Base pairs

9,168 bp

Sequence data

Purity

1. Contains over 70% double-stranded covalently closed circular form I (RFI).
2. Shown to retain cloning sites by dideoxy sequencing method.
3. Shown to be cleavage at a single site by restriction enzymes specific to these sites.

Usage

This is a vector for transformation of plants such as Arabidopsis, Tomato, Tobacco, Rice using Rhizobium (Agrobacterium) which is available for binary vector method.

Restriction enzyme map of pRI 909 and pRI 910 DNA

In pRI 909 and pRI 910, the sequence of MCS and its neighboring region is the opposite direction.

Cloning site