Description

This kit is a product to prepare template for 1 step real-time RT-PCR by simple method without RNA extraction process from cells cultured at 96 well-plates or others. This kit was developed to use only for One Step SYBR^® PrimeScript™ RT-PCR Kit (Perfect Real Time) (Cat. #RR066A/B) and One Step SYBR^® PrimeScript™ RT-PCR Kit II (Perfect Real Time) (Cat. #RR086A/B) . In combination with these kits, gene expression analysis can be performed about 2.5 hours (without DNase treatment, 2 hours) .
Templates which prepared directly from small quantities of cell by this kit is useful for analysis of gene expression profile without influencing high sensitivity of 1 step realtime RT-PCR. Because genomic DNA can be removed efficiently by treating DNase in this kit, this kit is powerful for expression analysis of gene, primers of which cannot be designed in both end of the exon junction, or which is expressing at low level.

Components

Cell Washing Buffer	12.5 ml×2
Cell Processing Buffer	8.0 ml
DNase I for Direct RNA Prep	200 µl
DNase I Buffer for Direct RNA Prep	1.0 ml×2

* This corresponds to 200 wells of 96 well plate.

Storage

-20°C

Cell Washing Buffer and Cell Processing Buffer can be stored at 4°C after thawing.
Avoid contamination.

Reagents not supplied in this kit

One Step SYBR^® PrimeScript™ RT-PCR Kit II (Perfect Real Time) (Cat. #RR086A/B)
One Step SYBR^® PrimeScript™ RT-PCR Kit (Perfect Real Time) (Cat. #RR066A/B)