Description

This product is a plasmid vector for producing a retrovirus vector with high transfection, expression efficiency.
By transfecting the vector into an appropriate packaging cell line, the vector expresses transient or stable transcribed product containing viruspackaging signal (ψ) and target gene and selective marker.
This vector possesses LTR and ψ (viral packaging signal), but not the structural gene necessary for particle formation and replication, which are gag, pol, and env, and this vector contains HCMV IE promoter within U3 region of 5' LTR and neomycin resistant gene as a selective marker.
HCMV IE promoter has high promoter activity, and can produce high-titer retroviruses. Also this vector contains intron with high ability of splicing, and it yields high transcription efficiency.

Storage

-20°C

Form

10 mM Tris-HCl (pH8.0), 1 mM EDTA

Chain length

pDON-5 Neo DNA	5,828 bp
pDON-5 DNA	4,697 bp

DNA Sequences of each Vector

pDON-5 Neo DNA (Fasta format text)
pDON-5 DNA (Fasta format text)

Preparation

Preparation cccDNA was purified by CsCl-EtBr ultracentrifugation.

Purity

1. Contains over 70% double-stranded covalently closed circular DNA(RFI) by agarose gel electrophoresis.
2. Confirmed to maintain cloning sites by dideoxy sequencing method.
3. Confirmed to be cleaved at a single site by restriction enzymes PmaC I, Apa I, Sac II,Not I, Bgl II, Cla I, BamH I, Sal I, Hpa I and Xho I.
(Xho I is pDON-5 only)

Application

Retrovirus-mediated gene transfer into mammalian cells.

Note

pDON-AI Vectors, pMEI-5 Vectors and pDON-5 Vectors are covered by U.S. Patent Nos. 6190907, 6451595 or their foreign counterparts, which are owned by ViroMed Inc. (KOREA) and are licensed to Takara Bio Inc.
The use of these products is limited for research purposes. It must not be used for clinical purpose or in vitro diagnosis. These products, any transformants or biomaterials obtained by using these products are prohibited to be transferred to third parties.
Users must contact TAKARA when they plan to use these products for purposes other than research use.
Any inquiries about the licenses relating to these products should be made to Takara Bio Inc. directly or through Takara Bio’s subsidiaries.