Description

Recombinant DNase I (RNase-free) is a recombinant from of bovine pancreatic DNase I. It is an endonuclease that catalyzes to the same extent the degradation of both single- and doublestranded DNA randomly,and produces 5'-P terminal oligonucleotides. As protease activity has been eliminated, this enzyme is stable around its optimum neutral pH range. So, this enzyme is suitable for RNA preparation at neutral pH.

Storage

-20°C

Concentration

5 units/µl

Form

20 mM	Tris-HCl, pH7.5 (at 4°C)
50 mM	NaCl
0.1 mM	CaCl2
50 ％	Glycerol

Source

Expressed in non-animal host carrying plasmid encodes the gene of bovine pancreas DNase I.

Supplied buffer (10×)

400 mM	Tris-HCl, pH7.5
80 mM	MgCl2
50 mM	DTT

Unit definition

One unit is the amount of the enzyme that increases the absorbance at 260 nm by 0.001 per minute at 25°C, pH5.0, with calf thymus DNA as the substrate (Kunitz unit).¹⁾

Purity

Ribonuclease activity is not detected after incubation of 1 μg of 16S, 23S rRNA with 10 units of this enzyme for 4 hours at 37°C, pH7.5.

Applications

• For digestion of template DNA without buffer exchange, after in vitro transcription with T7 or SP6 RNA Polymerase.⁵⁾
• For nick translation with DNA polymerase I (Cat.#2130)²⁾.
• Making a DNA library for shotgun sequencing in the presence of Mn²⁺. ³⁾
• For foot-printing analysis of the interaction between DNA and protein.⁴⁾
• For digestion of genomic DNA before RT-PCR.