Description

pRI 101 DNA series are binary vectors for expressing foreign gene in plant cells, which has the 35S promoter of cauliflower mosaic virus (CaMV) and a 5' non-coding region (5'-UTR) of alcohol dehydrogenase (ADH) gene. The 5'-UTR of ADH functions as a translational enhancer in plants¹⁾. There are two types of vectors, pRI 101-AN DNA having a 5'-UTR of Arabidopsis ADH (AtADH 5'-UTR) and pRI 101 ON DNA having a 5'-UTR of rice ADH (OsADH 5'-UTR). The pRI 101-AN is for dicotyledonous plants such as tobacco or Arabidopsis and the pRI 101-ON is for monocotyledonous plants such as rice.
The pRI 101 DNA are shuttle vectors, and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are high copy number plasmid because these have replication origin same as that of pUC type plasmid (ColE1 ori), and these are maintained stably in Rhizobium (Agrobacterium) also with mutant type replication origin of Ri plasmid (Ri-ori)²⁾. The pRI 101 DNA are possible to integrate target gene in plant chromosome stably because the cloning site of these products are located at the position closer to Right Border (RB) of T-DNA than the selection marker for plant, so the target gene is not deleted.

* These products were developed by TaKaRa Bio Inc. to which Nara Institute of Science and Technology provided supports and samples.

Components

pRI 101-AN DNA (Cat.#3262) 10 µg
pRI 101-ON DNA (Cat.#3263) 10 µg
(Concentration : 0.5 µg/µl)

Storage

-20°C

Base pairs

pRI 101-AN DNA	10,417 bp
pRI 101-ON DNA	10,454 bp

Sequence data

pRI 101-AN DNA (Fasta format text)
pRI 101-ON DNA (Fasta format text)

Purity

1. Contains over 70% double-stranded covalently closed circular form I (RFI).
2. Shown to retain cloning sites by dideoxy sequencing method.
3. Shown to be cleavage at a single site by restriction enzymes specific to these sites.

Usage

• pRI 101-AN DNA
  This is a vector for transformation of dicotyledonous plants such as Arabidopsis, Tomato, Tobacco, using Rhizobium (Agrobacterium) which is available for binary vector method.
• pRI 101-ON DNA
  This is a vector for transformation of monocotyledonous plants such as Rice, using Rhizobium (Agrobacterium) which is available for binary vector method.

Vector map

ColEi ori : Replication origin in E. coli
Ri ori : Replication origin in Rhizobium (Agrobacterium)
RB, LB : T-DNA border sequences
NOS-promoter, NOS-terminator : Promoter or terminator for expression of selectable marker gene in plant
NPTII : Selection marker gene in plant
AtADH 5'-UTR, OsADH 5'-UTR : 5' non-coding region of Arabidopsis or Rice Alcohol dehydrogenase gene (translational enhancer)
35S promoter : Cauliflower mosaic virus (CaMV) promoter for expression of target gene in plant
NPTIII : Selection marker gene in E. coli or Rhizobium (Agrobacterium)

Cloning site

pRI 101-AN DNA

pRI 101-ON DNA