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RT-PCR

PrimeScript™ II 1st strand cDNA Synthesis Kit (Cat. #6210A/B)

The 1st strand cDNA synthesis reactant can be used directly as PCR template without purification. However, the volume of the 1st strand reaction must be less than 1/10th of the total PCR reaction volume. Also, there are PCR enzymes for which the rate of amplification may be affected by the amount of starting template. Thus, please refer to PCR enzyme instruction to assess the appropriate amount of template to use.
In case of non-specific amplification or no product produced after PCR, results can be improved by treating the cDNA synthesis reaction with RNase H treatment.

[Recommended PCR enzymes]
For excellent efficient PCR：TaKaRa Ex Taq™, TaKaRa Ex Taq™ HS
For long PCR：TaKaRa LA Taq™, TaKaRa LA Taq™ HS
For Accurate PCR：PrimeSTAR™ HS DNA Polymerase