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Protocols

Westase(酵母細胞壁溶解酵素)

プロトプラスト調製条件例

菌株 培地 培養条件 プロトプラスト調製条件
Saccharomyces cerevisiae
Trigonopsis variabilis
YPG 25℃, 1 day
Shake culture
(reciprocate at 120 rpm)
0.5% Westase solution
0.5 M Na-tartrate
McIlvain buffer (pH6.0)
30℃, Reciprocal shaking, 1-2 hr
Lipomyces starkeyi
Candida utilis
YPG 25℃, 1 day
Shake culture
(reciprocate at 120 rpm)
0.5% Westase solution
0.4 M Na-tartrate
McIlvain buffer (pH6.0)
30℃, Reciprocal shaking, 1-2 hr
Shizosaccharomyces pombe
Hansenula mrakii
Kluyveromyces lactis
Pichia anomala
Filobasidium floriforme
Candida colliculosa
YPG 25℃, 1 day
Shake culture
(reciprocate at 120 rpm)
0.5% Westase solution
0.4 M Na-tartrate
McIlvain buffer (pH6.0)
30℃, Reciprocal shaking, 3-4 hr
Kloeckera apiculata
Cryptococcus albidus
YPG 25℃, 1 day
Shake culture
(reciprocate at 120 rpm)
0.5% Westase solution
0.3 M Na-tartrate
McIlvain buffer (pH6.0)
30℃, Reciprocal shaking, 1-2 hr
Ustilago maydis
Graphiola phoenicis
Brettanomyces bruxellensis
Phaffia rhodozyma
YPG 25℃, 2 day
Shake culture
(reciprocate at 120 rpm)
0.5% Westase solution
0.5 M Na-tartrate
McIlvain buffer (pH6.0)
30℃, Reciprocal shaking, 1-2 hr
Tremella mesenterica YPG 25℃, 2 day
Shake culture
(reciprocate at 120 rpm)
0.5% Westase solution
0.4 M Na-tartrate
McIlvain buffer (pH6.0)
30℃, Reciprocal shaking, 1-2 hr

YPG培地: 0.5%酵母エキス、1.0%ポリペプトン、1.0%グルコース、0.1%KH2PO4、0.05%MgSO4・7H2O, pH 5.5。
McIlvain Buffer: 0.1 Mクエン酸溶液と0.2 Mリン酸水素二ナトリウム溶液を約36.8:63.2で混合し、pH6.0に調整する。
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