標準的な制限酵素の反応時間は1時間であるが、長時間のインキュベーションを行うことがしばしばある。この表は、2、5、16時間後の残存活性(完全に残存:+、一部残存:±、残存活性なし:-、未試験:NT)を示している。
| 制限酵素 |
2時間 |
5時間 |
16時間 |
| Aat II |
+ |
+ |
+ |
| Acc I |
+ |
+ |
+ |
| Acc II |
+ |
+ |
+ |
| Acc III |
+ |
+ |
± |
| Afa I |
± |
± |
± |
| Afl II |
+ |
+ |
± |
| Alu I |
NT |
NT |
+ |
| Aor13H I |
+ |
+ |
+ |
| Aor51H I |
+ |
+ |
+ |
| Apa I |
+ |
+ |
+ |
| ApaL I |
NT |
NT |
+ |
| Bal I |
+ |
± |
- |
| BamH I |
+ |
+ |
+ |
| Ban II |
NT |
+ |
+ |
| BciT130 I |
+ |
+ |
± |
| Bcn I |
+ |
+ |
- |
| Bgl I |
NT |
+ |
- |
| Bgl II |
NT |
+ |
+ |
| Bln I |
+ |
+ |
± |
| BmeT110 I |
+ |
+ |
± |
| Bpu1102 I |
+ |
+ |
+ |
| BspT104 I |
+ |
+ |
+ |
| BspT107 I |
+ |
+ |
+ |
| Bsp1286 I |
+ |
+ |
+ |
| Bsp1407 I |
+ |
+ |
+ |
| BssH II |
+ |
+ |
+ |
| BstP I |
+ |
+ |
+ |
| BstX I |
+ |
+ |
+ |
| Cfr10 I |
+ |
± |
± |
| Cla I |
+ |
+ |
+ |
| Cpo I |
± |
- |
- |
| Dde I |
+ |
+ |
+ |
| Dra I |
+ |
- |
- |
| Eae I |
+ |
+ |
+ |
| EcoO65 I |
+ |
+ |
± |
| EcoO109 I |
+ |
+ |
± |
| EcoR I |
+ |
+ |
+ |
| EcoR V |
NT |
+ |
+ |
| EcoT14 I |
+ |
± |
- |
| EcoT22 I |
+ |
± |
- |
| Eco52 I |
± |
± |
- |
| Eco81 I |
+ |
+ |
+ |
| Fba I |
+ |
+ |
± |
| Fok I |
+ |
+ |
+ |
| Hae II |
+ |
+ |
+ |
| Hae III |
+ |
+ |
+ |
| Hap II |
+ |
+ |
+ |
| 制限酵素 |
2時間 |
5時間 |
16時間 |
| Hha I |
+ |
+ |
+ |
| Hinc II |
+ |
- |
- |
| Hind III |
+ |
+ |
+ |
| Hinf I |
NT |
+ |
+ |
| Hpa I |
+ |
+ |
+ |
| Kpn I |
+ |
+ |
+ |
| Mbo I |
+ |
+ |
+ |
| Mbo II |
± |
± |
± |
| Mfl I |
+ |
± |
± |
| Mlu I |
NT |
+ |
+ |
| Msp I |
+ |
+ |
± |
| Mun I |
NT |
+ |
+ |
| Nae I |
+ |
+ |
± |
| Nco I |
NT |
+ |
+ |
| Nde I |
+ |
+ |
+ |
| Nhe I |
+ |
+ |
+ |
| Not I |
+ |
+ |
+ |
| Nru I |
NT |
+ |
+ |
| PmaC I |
+ |
± |
- |
| Psp1406 I |
+ |
+ |
+ |
| Pst I |
NT |
+ |
+ |
| Pvu I |
+ |
+ |
± |
| Pvu II |
+ |
+ |
+ |
| RspRS II |
+ |
+ |
+ |
| Sac I |
+ |
+ |
+ |
| Sac II |
+ |
+ |
+ |
| Sal I |
+ |
± |
± |
| Sau3A I |
± |
± |
± |
| Sca I |
- |
- |
- |
| Sfi I |
+ |
+ |
+ |
| Sma I |
+ |
+ |
+ |
| Smi I |
+ |
+ |
+ |
| SnaB I |
+ |
+ |
- |
| Spe I |
NT |
+ |
+ |
| Sph I |
- |
- |
NT |
| Sse8387 I |
+ |
+ |
+ |
| Ssp I |
NT |
+ |
± |
| Stu I |
+ |
± |
± |
| Taq I |
+ |
+ |
+ |
| Van91 I |
+ |
+ |
- |
| VpaK11B I |
+ |
+ |
+ |
| Xba I |
+ |
+ |
+ |
| Xho I |
+ |
+ |
+ |
| Xsp I |
+ |
+ |
± |
+:完全に残存 ±:一部残存 -:残存活性なし NT:未試験