Isoschizomer一覧表

■アイソシゾマー(isoschizomer)とは
アイソシゾマーは同じ塩基配列を認識する制限酵素です。弊社ウェブカタログやデータシートで制限酵素名のあとに( )で表記している酵素はアイソシゾマーです。
認識配列は同じですが、切断位置やメチル化の影響などは異なる場合があります。
切断位置は下記一覧でご確認ください。

弊社で販売している制限酵素のメチル化の影響はこちらをご覧ください。
* RA or G KG or T HA or C or T
YC or T MA or C VA or C or G
WA or T BC or G or T NA or C or G or T
SG or C DA or G or T

認識部位は5'→3'の方向に一本鎖のみ書いており、切断点は矢印(↓)で示した。矢印がないものは、正確な切断点が判明していないことを示す。
Eco31 IやFok I等のように認識部位と切断点が離れている酵素は、切断点をカッコ内に示した。
例としてEco31 I GGTCTC(1/5)の切断点を下に示す。
5' GGTCTCN↓    3'
3' CCAGAGN NNNN↑5'
Nci I は、3'-リン酸になるように切断する。
表中にボールドで示した制限酵素はプロトタイプ(その塩基配列の認識・切断を最初に確認された制限酵素)です。
は、タカラバイオで販売している酵素です。

※一覧は2004年現在の情報を元に作成したものです。
制限酵素 認識配列*
Aar I CACCTGC(4/8)
Aat II
Ppu1253 I
Ssp5230 I
Zra I
GACGT↓C
GACGTC
GACGT↓C
GAC↓GTC
Acc I
Dsa VI
Fbl I
Xmi I
GT↓MKAC
GTMKAC
GT↓MKAC
GT↓MKAC
Ace III CAGCTC(7/11)
Aci I CCGC(-3/-1)
Acl I
Psp1406 I
AA↓CGTT
AA↓CGTT
Acy I
Aha II
Aos II
AstW I
Asu III
Bbi II
BsaH I
BstAC I
Hgi I
HgiD I
HgiG I
HgiH II
Hin1 I
Hin8 I
Hsp92 I
Msp17 I
NlaS II
Pam II
SspJ II
SspM1 II
SspM2 II
Uba1381 I
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GR↓CGYC
GRCGYC
GR↓CGYC
GR↓CGYC
GRCGYC
GR↓CGYC
GRCGYC
GRCGYC
GRCGYC
GRCGYC
Afl II
Bfr I
BsaF I
BspT I
Bst98 I
Cfr92 I
Esp4 I
MspC I
Uba1265 I
Uba1266 I
Uba1299 I
Uba1312 I
Uba1313 I
Uba1331 I
Uba1374 I
Uba1420 I
Uba1426 I
Uba1443 I
Vfi I
Vha464 I
C↓TTAAG
C↓TTAAG
CTTAAG
C↓TTAAG
C↓TTAAG
CTTAAG
C↓TTAAG
C↓TTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
CTTAAG
C↓TTAAG
Afl III A↓CRYGT
Age I
AsiA I
AsiG I
BshT I
CspA I
PinA I
A↓CCGGT
A↓CCGGT
A↓CCGGT
A↓CCGGT
A↓CCGGT
A↓CCGGT
Aha III
Dra I
TTT↓AAA
TTT↓AAA
Alf I GCANNNNNN↓TGC
Alo I (7/12)GAACNNNNNNTCC(12/7)
Alu I
BsaL I
Mlt I
Otu I
OtuN I
Oxa I
Uba1433 I
Uba1441 I
AG↓CT
AGCT
AG↓CT
AGCT
AGCT
AGCT
AGCT
AGCT
AlwN I
CaiI
CAGNNN↓CTG
CAGNNN↓CTG
Apa I
Bsp120 I
EciE I
PspOM I
Psp30 I
Uba1156 I
Uba1157 I
Uba1165 I
Uba1202 I
Uba1241 I
Uba1368 I
GGGCC↓C
G↓GGCCC
GGGCCC
G↓GGCCC
GGGCCC
GGGCCC
GGGCCC
GGGCCC
GGGCCC
GGGCCC
GGGCCC
ApaB I
BstAP I
GCANNNNN↓TGC
GCANNNN↓NTGC
ApaL I
Aaq I
Alw44 I
Ame I
Bsp146 I
Pfl23 I
Pfr12 I
Pli I
Sno I
Uba44 I
Uba1203 I
Uba1387 I
Vne I
G↓TGCAC
GTGCAC
G↓TGCAC
GTGCAC
GTGCAC
GTGCAC
GTGCAC
GTGCAC
G↓TGCAC
G↓TGCAC
GTGCAC
GTGCAC
G↓TGCAC
Apo I
Acs I
Fsi I
XapI
R↓AATTY
R↓AATTY
R↓AATTY
R↓AATTY
Asc I
PalA I
Sgs I
GG↓CGCGCC
GG↓CGCGCC
GG↓CGCGCC
AspCN I GCCGC
Asu I
AhaB1 I
Apu I
AspS9 I
Avc I
Bac36 I
Bal228 I
BavA II
BavB II
Bce22 I
BmgT120 I
BsaS I
BshK I
BsiZ I
BspB II
BspF4 I
Bsp1894 I
Bsu54 I
CfrN I
Cfr4 I
Cfr8 I
Cfr13 I
Cfr23 I
Cfr33 I
Cfr45 I
Cfr46 I
Cfr47 I
Cfr52 I
Cfr54 I
Eco39 I
Eco47 II
Eco196 II
Eco201 I
Fmu I
Gse I
Hin5 II
Mja II
Msp24 I
MthB I
MthT I
NlaD II
NmuE II
NmuS I
Nsp IV
NspL II
Nsp7121 I
Pde12 I
Pph1579 I
Pph1773 I
Pse I
Psp I
SauB I
Sau2 I
Sau5 I
Sau13 I
Sau14 I
Sau17 I
Sau96 I
Sdy I
Uba1099 I
Uba1134 I
Uba1160 I
Uba1164 I
Unb I
VpaK15 I
VpaK25 I
G↓GNCC
GGNCC
GGNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
GGNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
G↓GNCC
GGNCC
GGNCC
GGNCC
G↓GNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNC↓C
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
G↓GNCC
GGNCC
GGNCC
G↓GNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
G↓GNCC
GGNCC
GGNCC
GGNCC
GGNCC
GGNCC
↓GGNCC
GGNCC
GGNCC
Asu II
Aca I
Acp I
Asp10H I
Avi I
Bim I
Bim19 I
Bpu14 I
BsiC I
BspT104 I
Bsp82 I
Bsp119 I
Bsp148 I
BstB I
Cbi I
Csp45 I
Fsp II
Lsp I
Mla I
Nsp V
NspB I
NspF I
NspJ I
Nsp29132 I
Osp i
Pla II
Sfu I
Sgr1839 I
SspRF I
Ssp1 I
Ssp48 I
Ssp152 I
Uba1385 I
Uba1452 I
TT↓CGAA
TTCGAA
TT↓CGAA
TT↓CGAA
TTCGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TTCGAA
TT↓CGAA
TTCGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TT↓CGAA
TTCGAA
TTCGAA
TTCGAA
TTCGAA
TTCGAA
TT↓CGAA
TT↓CGAA
TTCGAA
TT↓CGAA
TT↓CGAA
TTCGAA
TT↓CGAA
TTCGAA
TTCGAA
Ava I
Acr I
Ama87 I
Aqu I
AspB I
AspC I
AspD I
Avr I
Bco I
BmeT110 I
BsiHKC I
BsoB I
BspLU4 I
BstS I
Bst7Q I
Eco27k I
Eco88 I
EspHK29 I
Nli I
Nli3877 I
NmuA I
Nsp III
NspD I
NspE I
NspSA I
Uba1205 II
Uba1436 I
Uba1440 I
Umi5 I
C↓YCGRG
CYCGRG
C↓YCGRG
C↓YCGRG
CYCGRG
CYCGRG
CYCGRG
CYCGRG
C↓YCGRG
C↓YCGRG
C↓YCGRG
C↓YCGRG
C↓YCGRG
C↓YCGRG
CYCGRG
C↓YCGRG
C↓YCGRG
CYCGRG
CYCGRG
CYCGR↓G
CYCGRG
C↓YCGRG
CYCGRG
CYCGRG
C↓YCGRG
CYCGRG
CYCGRG
CYCGRG
CYCGRG
Ava II
Afl I
AspB II
AspC II
AspD II
Asp697 I
Asp745 I
BamNx I
Bme18 I
Bme216 I
BspF53 I
BspJ105 I
Bsp71 I
Bsp100 I
Bsp128 I
Bsp132 I
Bsp133 I
Bsp1260 I
BsrA I
Bst4Q I
BthA I
Bti I
Cau I
Cli I
Clm II
Dsa1 V
EagM I
Eco47 I
Erp I
Fdi I
FspMS I
GspA I
HgiB I
HgiC II
HgiE I
HgiH III
HgiJ I
Hsp2 I
Kzo49 I
Mfo I
Mli I
MspA I
Nli II
Nli3877 II
NmuA II
NspD II
NspG I
NspH II
NspK I
Pfl19 I
Psp03 I
Sfn I
Sgh I
Sin I
SinA I
SinB I
SinC I
SinD I
SinE I
SinF I
SinG I
SinH I
SinJ I
SmuE I
Syn I
Tru I
Uba48 I
Uba62 I
Uba1131 I
G↓GWCC
G↓GWCC
GGWCC
GGWCC
GGWCC
GGWCC
G↓GWCC
G↓GWCC
G↓GWCC
G↓GWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
G↓GWCC
GGWCC
G↓GWCC
GGWCC
GGWCC
G↓GWCC
GGWCC
G↓GWCC
G↓GWCC
G↓GWCC
G↓GWCC
GGWCC
G↓GWCC
G↓GWCC
G↓GWCC
G↓GWCC
G↓GWCC
GGWCC
G↓GWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWC↓C
GGWCC
GGWCC
G↓GWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
G↓GWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
Uba1249 I
Uba1272 I
Uba1278 I
Uba1304 I
Uba1314 I
Uba1373 I
Uba1413 I
Uba1438 I
VpaK11 I
VpaK11A I
VpaK11B I
VpaK65 I
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
GGWCC
↓GGWCC
G↓GWCC
GGWCC
Ava III
BfrB I
BfrC I
EcoT22 I
Mph1103 I
Nsi I
PinB I
Ppu10 I
Sep I
SmuC I
Uba1353 I
Uba1367 I
Uba1384 I
Zsp2 I
ATGCAT
ATG↓CAT
ATGCAT
ATGCA↓T
ATGCA↓T
ATGCA↓T
ATGCA↓T
A↓TGCAT
ATGCA↓T
ATGCAT
ATGCAT
ATGCAT
ATGCAT
ATGCA↓T
Avr II
AspA2 I
AvrB II
Bln I
Bsp2A I
XmaJ I
C↓CTAGG
C↓CTAGG
C↓CTAGG
C↓CTAGG
C↓CTAGG
C↓CTAGG
Bae I (10/15)ACNNNNGTAYC(12/7)
Bal I
Mls I
MluN I
Mlu31 I
Msc I
Msp20 I
TGG↓CCA
TGG↓CCA
TGG↓CCA
TGG↓CCA
TGG↓CCA
TGG↓CCA
BamH I
Aac I
Aae I
Aca II
AccEB I
Ain II
Ali II
Ali12257 I
Ali12258 I
ApaC I
AspT II
Atu1 II
BamF I
BamK I
BamN I
Bca1259 I
Bce751 I
Bco10278 I
Bna I
BsaD I
Bsp30 I
Bsp46 I
Bsp98 I
Bsp130 I
Bsp131 I
Bsp144 I
Bst I
BstQ I
Bst1126 I
Bst2464 I
Bst2902 I
Bst8565 I
Bst8566 I
Bsu90 I
Bsu8646 I
Cel I
Dds I
Gdo I
Gin I
Gox I
Gse III
Mle I
Mlu23 I
NasB I
NspSA IV
Nsp29132 II
Pac1110 I
Par177 I
Psp56 I
Rhs I
Rlu4 I
Sur I
Uba19 I
Uba31 I
Uba38 I
Uba51 I
Uba88 I
Uba1098 I
Uba1163 I
Uba1167 I
Uba1172 I
Uba1173 I
Uba1205 I
Uba1224 I
Uba1242 I
Uba1250 I
Uba1258 I
Uba1297 I
Uba1302 I
Uba1324 I
Uba1325 I
Uba1334 I
Uba1339 I
Uba1346 I
Uba1383 I
Uba1398 I
Uba1402 I
Uba1414 I
G↓GATCC
GGATCC
GGATCC
GGATCC
G↓GATCC
GGATCC
G↓GATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
G↓GATCC
GGATCC
G↓GATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
G↓GATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GG↓ATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
G↓GATCC
GGATCC
G↓GATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
G↓GATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
GGATCC
Bbv I
AlwX I
BsaU I
BseX I
Bsp423 I
BsrV I
BsrV1 I
Bst12 I
Bst71 I
Lsp1109 I
GCAGC(8/12)
GCAGC(8/12)
GCAGC
GCAGC(8/12)
GCAGC(8/12)
GCAGC
GCAGC(8/12)
GCAGC(8/12)
GCAGC(8/12)
GCWGC
Bbv II
Bbr7 I
Bbs I
Bbv16 II
Bco102 II
Bpi I
BpuA I
BsaVI
Bsc91 I
Bsp VI
Bst V2I
GAAGAC(2/6)
GAAGAC(7/11)
GAAGAC(2/6)
GAAGAC(2/6)
GAAGAC
GAAGAC(2/6)
GAAGAC(2/6)
GAAGAC
GAAGAC(2/6)
GAAGAC
GAAGAC(2/6)
BbvC I CC↓TCAGC
Bcc I CCATC
Bcef I
BceA I
ACGGC(12/13)
ACGGC(12/14)
Bce83 I
BpuE I
CTTGAG(16/14)
CTTGAG(16/14)
Bcg I (10/12)GCANNNNNNTCG(12/10)
BciV I
Bfu I
GTATCC(6/5)
GTATCC(6/5)
Bcl I
AtuC I
Bco102 I
BsiQ I
BspX II
BstG I
BstK I
Bst77 I
Cpe I
Cth I
Fba I
Ksp22 I
Pov I
Sse I
Sst IV
Uba1282 I
Uba1283 I
Uba1431 I
Uba1447 I
Umi7 I
T↓GATCA
TGATCA
TGATCA
T↓GATCA
T↓GATCA
TGATCA
TGATCA
TGATCA
TGATCA
TGATCA
T↓GATCA
T↓GATCA
T↓GATCA
TGATCA
TGATCA
TGATCA
TGATCA
TGATCA
TGATCA
TGATCA
Bet I
Bca77 I
BsaW I
Bst1473 I
W↓CCGGW
W↓CCGGW
W↓CCGGW
WCCGGW
Bfi I
BmrI
ACTGGG(5/4)
ACTGGG(5/4)
Bgl I
Van I
GCCNNNN↓NGGC
GCCNNNNNGGC
Bgl II
Ncr I
NspMAC I
Pae2k I
Pae18k I
A↓GATCT
A↓GATCT
A↓GATCT
A↓GATCT
A↓GATCT
Bin I
AclW I
Alw I
BspP I
BsrW I
Bth II
Ral8 I
GGATC(4/5)
GGATC(4/5)
GGATC(4/5)
GGATC(4/5)
GGATC
GGATC
GGATC
Bmg I GKGCCC
Bpl I (8/13)GAGNNNNNCTC(13/8)
Bpu10 I CCTNAGC(-5/-2)
BsaA I
BstBA I
MspY I
Ppu6 I
Ppu11 I
Ppu21 I
YAC↓GTR
YAC↓GTR
YAC↓GTR
YACGTR
YACGTR
YACGTR
BsaB I
Bco63 I
BseJ I
Bse8 I
Bsh1365 I
BsiB I
BsrBR I
Mam I
GATNN↓NNATC
GATNNNNATC
GATNN↓NNATC
GATNN↓NNATC
GATNN↓NNATC
GATNN↓NNATC
GATNN↓NNATC
GATNN↓NNATC
BsaX I (9/12)ACNNNNNCTCC(10/7)
Bsb I CAACAC
BscG I
BpuJ I
CCCGT
CCCGT
BseM II
BspCN I
CTSAG(10/8)
CTCAG(9/7)
BseP I
BscE I
BsoP I
BsrH I
BssH II
CfrJ5 I
Eco143 I
Eco152 I
Esp7 I
Esp8 I
Kpn30 I
Pau I
Uba69 I
G↓CGCGC
GCGCGC
GCGCGC
GCGCGC
G↓CGCGC
GCGCGC
GCGCGC
GCGCGC
GCGCGC
GCGCGC
GCGCGC
G↓CGCGC
GCGCGC
BseR I GAGGAG(10/8)
BseS I
Bme1580 I
BseS I
GKGCM↓C
GKGCM↓C
GKGCM↓C
BseY I C↓CCAGC
Bsg I GTGCAG(16/14)
Bsi I
BssS I
Bst2B I
CTCGTG(-5/-1)
CTCGTG(-5/-1)
CTCGTG(-5/-1)
BsiY I
Bsc4 I
Bsc107 I
BseL I
Bse23 I
Bsl I
CCNNNNN↓NNGG
CCNNNN↓NNNGG
CCNNNNNNNGG
CCNNNNN↓NNGG
CCNNNNNNNGG
CCNNNNN↓NNGG
Bsm I
Asp26H I
Asp27H I
Asp35H I
Asp36H I
Asp40H I
Asp50H I
BsaM I
BscC I
Mva1269 I
Pct I
Uba1382 I
Uba1415 I
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC(1/-1)
GAATGC
GAATGC
BsmA I
Alw26 I
BsoMA I
GTCTC(1/5)
GTCTC(1/5)
GTCTC(1/5)
BspG I CTGGAC
BspH I
Pag I
Rca I
Rhc I
RspX I
T↓CATGA
T↓CATGA
T↓CATGA
TCATGA
TCATGA
BspLU11 I
Pci I
Psc I
A↓CATGT
A↓CATGT
A↓CATGT
BspM I
Acc36 I
BfuA I
ACCTGC(4/8)
ACCTGC(4/8)
ACCTGC(4/8)
BspM II
Acc III
Aor13H I
Bbf7411 I
BbvA III
Bla7920 I
BlfI
BseA I
BsiM I
BspE I
Bsp13 I
Bsu22 I
Bsu23 I
CauB3 I
Cfr57 I
EspHK26 I
Kpn2 I
Mro I
PinB II
Pta I
Uba1136 I
Uba1279 I
Uba1375 I
Uba1425 I
T↓CCGGA
T↓CCGGA
T↓CCGGA
TCCGGA
T↓CCGGA
TCCGGA
T↓CCGGA
T↓CCGGA
T↓CCGGA
T↓CCGGA
T↓CCGGA
TCCGGA
T↓CCGGA
T↓CCGGA
TCCGGA
TCCGGA
T↓CCGGA
T↓CCGGA
T↓CCGGA
T↓CCGGA
TCCGGA
TCCGGA
TCCGGA
TCCGGA
BspNC I CCAGA
Bsp24 I (8/13)GACNNNNNNTGG(12/7)
Bsp1407 I
Aau I
BsrG I
BstAU I
Bst170 I
SspB I
T↓GTACA
T↓GTACA
T↓GTACA
T↓GTACA
TGTACA
T↓GTACA
制限酵素 認識配列*
Bsr I
BscH I
BseN I
Bse1 I
BsrS I
Bst11 I
Tsp1 I
ACTGG(1/-1)
ACTGG
ACTGG(1/-1)
ACTGG(1/-1)
ACTGG(1/-1)
ACTGG(1/-1)
ACTGG(1/-1)
BsrB I
AccBS I
Mbi I
GAGCGG(-3/-3)
GAGCGG(-3/-3)
CCGCTC(-3/-3)
BsrD I
Bse3D I
BseM I
GCAATG(2/0)
GCAATG(2/0)
GCAATG(2/0)
BstE II
Acr II
AspA I
Bse59 I
Bse64 I
BstD I
BstP I
Bst31 I
Cfr7 I
Cfr19 I
Eca I
Eci125 I
EcoO65 I
EcoO128 I
Eco91 I
Kox I
NspSA II
PspE I
SciA I
Uba1291 I
G↓GTNACC
G↓GTNACC
G↓GTNACC
GGTNACC
GGTNACC
GGTNACC
G↓GTNACC
GGTNACC
GGTNACC
GGTNACC
G↓GTNACC
G↓GTNACC
G↓GTNACC
G↓GTNACC
G↓GTNACC
G↓GTNACC
G↓GTNACC
G↓GTNACC
GGTNACC
GGTNACC
BstX I
BscJ I
BssG I
BstT I
CCANNNNN↓NTGG
CCANNNNNNTGG
CCANNNNNNTGG
CCANNNNNNTGG
BtgZ I GCGATG(10/14)
Btr I
BmgB I
CACGTC(-3/-3)
CACGTC(-3/-3)
Bts I GCAGTG(2/0)
Cac8 I
BstC8 I
GCN↓NGC
GCN↓NGC
Cau II
Aha I
Ase II
Asp1 I
AsuC2 I
Bcn I
BspF105 I
BspJ67 I
Bsp7 I
Bsp8 I
Bsp55 I
EciD I
EcoH I
Eco121 I
Eco179 I
Eco190 I
HgiS21 I
HgiS22 I
Hin3 I
Kpn49k II
Nci I
Pae181 I
Rsh II
Ssp2 I
Tmu1 I
Uba41 I
Uba42 I
Uba1280 I
Uba1318 I
Uba1347 I
Uba1370 I
Uba1372 I
Uba1376 I
Uba1378 I
Uba1389 I
Uba1401 I
Uba1423 I
Uba1424 I
CC↓SGG
CC↓SGG
CCSGG
CCSGG
CC↓SGG
CC↓SGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
↓CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CC↓SGG
CCSGG
↓CCSGG
CC↓SGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
CCSGG
Cdi I CATCG
Cfr I
Cfr14 I
Cfr38 I
Cfr39 I
Cfr40 I
Cfr55 I
Cfr59 I
Eae I
EciB I
EcoHA I
Eco90 I
Eco164 I
EspHK16 I
EspHK24 I
KspHK15 I
Uba36 I
Uba1188 I
Uba1327 I
Y↓GGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
Y↓GGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
YGGCCR
Cfr10 I
Bco118 I
Bse118 I
Bsp21 I
BsrF I
BssA I
R↓CCGGY
R↓CCGGY
R↓CCGGY
RCCGGY
R↓CCGGY
R↓CCGGY
Cje I (8/14)CCANNNNNNGT(15/9)
CjeN II GAGNNNNNGT
CjeP I (7/13)CCANNNNNNNTC(14/8)
Cla I
Aag I
Apu16 I
Asp707 I
Ban III
BavC I
BbvA II
BciB I
Bci29 I
Bcm I
Bdi I
BfrA I
BliR I
Bli41 I
Bli86 I
Bsa29 I
Bsc I
BseC I
BsiX I
BspD I
BspJ II
BspX I
Bsp2 I
Bsp4 I
Bsp84 I
Bsp106 I
Bsp125 I
Bsp126 I
Bsp127 I
Bsp145 I
BsrC I
BstLV I
Bst28 I
Bsu15 I
BsuTU I
Btu I
Csp4 I
Lpl I
Pga I
Rme21 I
Spm I
Uba22 I
Uba24 I
Uba30 I
Uba34 I
Uba43 I
Uba1096 I
Uba1100 I
Uba1133 I
Uba1137 I
Uba1138 I
Uba1144 I
Uba1145 I
Uba1161 I
Uba1168 I
Uba1195 I
Uba1196 I
Uba1197 I
Uba1198 I
Uba1199 I
Uba1200 I
Uba1233 I
Uba1238 I
Uba1246 I
Uba1257 I
Uba1275 I
Uba1286 I
Uba1295 I
Uba1315 I
Uba1342 I
Uba1366 II
Uba1379 I
Uba1380 I
Uba1394 I
Uba1412 I
Uba1416 I
Uba1427 I
Uba1430 I
Uba1451 I
Uba1453 I
Zho I
AT↓CGAT
AT↓CGAT
ATCGAT
ATCGAT
AT↓CGAT
ATCGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
ATCGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
ATCGAT
ATCGAT
ATCGAT
AT↓CGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
ATCGAT
ATCGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
AT↓CGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
ATCGAT
AT↓CGAT
CspC I (11/13)CAANNNNNGTGG(12/10)
CctM I AAGGAG(20/18)
CviJ I
CviK I
CviL I
CviM I
CviN I
CviO I
CviT I
RG↓CY
RGCY
RGCY
RGCY
RGCY
RGCY
RG↓CY
CviR I
HpyCH4 V
TG↓CA
TG↓CA
Dde I
BstDE I
HpyF3 I
C↓TNAG
C↓TNAG
C↓TNAG
Dpn I
Cfu I
Mal I
Mph1103 II
Nan II
NgoDX IV
NmuD I
NmuE I
NsuD I
G6mA↓TC
G6mA↓TC
G6mA↓TC
G6mATC
G6mATC
G6mATC
G6mATC
G6mATC
Dra II
EcoO109 I
Pss I
Uba1326 I
VneA I
RG↓GNCCY
RG↓GNCCY
RGGNC↓CY
RGGNCCY
RGGNCCY
Dra III
Ade I
CACNNN↓GTG
CACNNN↓GTG
Drd I
Aas I
DseD I
GACNNNN↓NNGTC
GACNNNN↓NNGTC
GACNNNN↓NNGTC
Drd II GAACCA
Dsa I
BstDS I
Btg I
C↓CRYGG
C↓CRYGG
C↓CRYGG
Eam1105 I
Ahd I
AspE I
EclHK I
NruG I
Uba1190 I
Uba1191 I
GACNNN↓NNGTC
GACNNN↓NNGTC
GACNNN↓NNGTC
GACNNN↓NNGTC
GACNNN↓NNGTC
GACNNN↓NNGTC
GACNNN↓NNGTC
Eci I
BpuC I
GGCGGA(11/9)
GGCGGA
EcoN I
BstEN I
BstW I
Uba1268 I
Uba1289 I
Uba1290 I
Uba1308 I
Uba1309 I
Uba1310 I
Xag I
CCTNN↓NNNAGG
CCTNN↓NNNAGG
CCTNNNNNAGG
CCTNNNNNAGG
CCTNNNNNAGG
CCTNNNNNAGG
CCTNNNNNAGG
CCTNNNNNAGG
CCTNNNNNAGG
CCTNN↓NNNAGG
EcoR I
Eco82 I
Eco159 I
Eco228 I
Eco237 I
Eco252 I
Fun II
Hal I
Kpn49k I
Rsr I
Sso I
Uba58 I
Van91 II
G↓AATTC
GAATTC
GAATTC
GAATTC
GAATTC
GAATTC
G↓AATTC
G↓AATTC
G↓AATTC
G↓AATTC
G↓AATTC
GAATTC
GAATTC
EcoR II
Acc38 I
Aeu I
Ajn I
Aor I
ApaOR I
Apy I
Asp2H I
Atu II
AtuB I
Atu1 I
BciT130 I
BinS I
Bpt I
BsaN I
BseB I
Bse16 I
Bse17 I
Bse24 I
BshG I
Bsi VI
BsiL I
BsoG I
BspN I
Bsp56 I
Bsp103 I
Bsp317 I
BstG II
BstM6 I
BstN I
BstO I
Bst1 I
Bst2 I
Bst2U I
Bst7Q II
Bst38 I
Bst100 I
BthD I
BthE I
Cdi27 I
CfrS37 I
Cfr5 I
↓CCWGG
CCWGG
CCWGG
↓CCWGG
CC↓WGG
CC↓WGG
CC↓WGG
CCWGG
CC↓WGG
CCWGG
CCWGG
CC↓WGG
CCWGG
CC↓WGG
CCWGG
CC↓WGG
CC↓WGG
CC↓WGG
CC↓WGG
CC↓WGG
CCWGG
CC↓WGG
CCWGG
CC↓WGG
CCWGG
CCWGG
CCWGG
CCWGG
CC↓WGG
CC↓WGG
CC↓WGG
CCWGG
CC↓WGG
CC↓WGG
CCWGG
CC↓WGG
CC↓WGG
CC↓WGG
CC↓WGG
CCWGG
CCWGG
CCWGG
制限酵素 認識配列*
Cfr11 I
Cfr20 I
Cfr22 I
Cfr24 I
Cfr25 I
Cfr27 I
Cfr28 I
Cfr29 I
Cfr30 I
Cfr31 I
Cfr35 I
Cfr58 I
Cth II
EagK I
Eca II
Ecl II
EclS39 I
Ecl66 I
Ecl136 I
Ecl137 II
Eco38 I
Eco40 I
Eco41 I
Eco60 I
Eco61 I
Eco67 I
Eco70 I
Eco71 I
Eco128 I
Eco136 I
Eco165 I
Eco170 I
Eco193 I
Eco206 I
Eco207 I
Eco254 I
Eco256 I
Ese6 II
EspHK7 I
EspHK22 I
EspHK30 I
Esp2 I
Esp24 I
Fsp1604 I
Hhd I
Kox165 I
Kpn10 I
Kpn13 I
Kpn14 I
Kpn16 I
KspHK12 I
KspHK14 I
Mlu2300 I
Mph I
Mva I
PspG I
Psp6 I
Sau16 I
Scg2 I
Sfl2a I
Sfl2b I
Sgr II
Sgr20 I
Sle I
Sni I
Ssl I
SspA I
Sth117 I
TaqX I
TspA I
Uba11 I
Uba13 I
Uba20 I
Uba81 I
Uba82 I
Uba1114 I
Uba1118 I
Uba1120 I
Uba1121 I
Uba1125 I
Uba1171 I
Uba1181 I
Uba1185 I
Uba1189 I
Uba1193 I
Uba1218 I
Uba1243 I
Uba1410 I
Uba1428 I
Zan I
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CC↓WGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CC↓WGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CC↓WGG
↓CCWGG
↓CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CC↓WGG
CC↓WGG
CCWGG
CC↓WGG
CC↓WGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CCWGG
CC↓WGG
EcoR V
BshL I
BsoA I
Bsp16 I
BstR I
Ceq I
Eco32 I
Eco178 I
Hja I
Nan I
NflA I
NsiC I
Pac1110 II
Pfl16 I
Uba1400 I
GAT↓ATC
GATATC
GATATC
GATATC
GATATC
GAT↓ATC
GAT↓ATC
GATATC
GATATC
GATATC
GATATC
GATATC
GATATC
GATATC
GATATC
Eco31 I
Bli49 I
Bli5508 I
Bsa I
Bso31 I
BspTN I
Cfr56 I
EcoA4 I
Eco42 I
Eco51 I
Eco95 I
Eco97 I
Eco101 I
Eco120 I
Eco127 I
Eco129 I
Eco155 I
Eco156 I
Eco157 I
Eco162 I
Eco185 I
Eco191 I
Eco203 I
Eco204 I
Eco205 I
Eco217 I
Eco225 I
Eco233 I
Eco239 I
Eco240 I
Eco241 I
Eco246 I
Eco247 I
Eco263 I
Ppa I
Rle69 I
Sau12 I
Uba65 I
Uba84 I
Uba1316 I
Uba1343 I
GGTCTC(1/5)
GGTCTC
GGTCTC
GGTCTC(1/5)
GGTCTC(1/5)
GGTCTC(1/5)
GGTCTC
GGTCTC(1/5)
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
GGTCTC
Eco47 III
Afe I
Ait I
Aor51H I
Fun I
AGC↓GCT
AGC↓GCT
AGCGCT
AGC↓GCT
AGC↓GCT
Eco57 I
Acu I
Bsp6 II
Eco112 I
Eco125 I
Fsf I
CTGAAG(16/14)
CTGAAG(16/14)
CTGAAG
CTGAAG
CTGAAG
CTGAAG
Eco57M I CTGRAG(16/14)
Esp I
Blp I
Bpu 1102 I
Bsp 1720 I
Cel II
Uba 1221 I
Uba 1222 I
Uba 1284 I
Uba 1320 I
GC↓TNAGC
GC↓TNAGC
GC↓TNAGC
GC↓TNAGC
GC↓TNAGC
GCTNAGC
GCTNAGC
GCTNAGC
GCTNAGC
Esp3 I
BsmB I
Esp16 I
Esp23 I
CGTCTC(1/5)
CGTCTC(1/5)
GAGACG
GAGACG
Fal I (8/13)AAGNNNNNCTT(13/8)
Fau I
Smu I
CCCGC(4/6)
CCCGC(4/6)
Fin I
BpuS I
BslF I
BsmF I
GGGAC
GGGAC(10/14)
(11/15)GGGAC(10/14)
GGGAC(10/14)
FnuD II
Acc II
BceR I
Bep I
Bpu95 I
Bsh1236 I
BspJ76 I
Bsp50 I
Bsp70 I
BstFN I
BstU I
BsuE II
Bsu1192 II
Bsu1193 I
Bsu1532 I
Bsu6633 I
Btk I
CpaA I
Cpa1150 I
FspM I
Hin1056 I
MvaA I
Mvn I
PflA I
SceA I
Sel I
Tha I
Tma I
Uba1321 I
Uba1404 I
Uba1405 I
Uba1446 I
CG↓CG
CG↓CG
CGCG
CGCG
CG↓CG
CG↓CG
CGCG
CGCG
CGCG
CG↓CG
CG↓CG
CGCG
CGCG
CGCG
CG↓CG
CGCG
CG↓CG
CGCG
CGCG
CGCG
CGCG
CGCG
CG↓CG
CGCG
CGCG
↓CGCG
CG↓CG
CGCG
CGCG
CGCG
CGCG
CGCG
Fnu4H I
Bis I
BsoF I
Bsp6 I
BssF I
BssX I
BthC I
Cac824 I
Fbr I
Fsp4H I
Isp I
Ita I
Sat I
Uur960 I
GC↓NGC
GC↓NGC
GC↓NGC
GC↓NGC
GCNGC
GCNGC
GCNG↓C
GCNGC
GC↓NGC
GC↓NGC
GC↓NGC
GC↓NGC
GC↓NGC
GC↓NGC
Fok I
BseG I
BstF5 I
HinGU II
Sts I
GGATG(9/13)
GGATG(2/0)
GGATG(2/0)
GGATG
GGATG(10/14)
Fse I GGCCGG↓CC
FspA I RTGC↓GCAY
Gdi II YGGCCG(-5/-1)
Gsu I
Bco35 I
Bpm I
BspJ74 I
Bsp22 I
Bsp28 I
Uba1437 I
Uba1444 I
CTGGAG(16/14)
CTGGAG
CTGGAG(16/14)
CTGGAG
CTGGAG
CTGGAG
CTGGAG
CTGGAG
Hae I WGG↓CCW
Hae II
AccB2 I
Bme142 I
BsmH I
Bsp143 II
BstH2 I
Btu34 II
HinH I
Lpn I
Ngo I
RGCGC↓Y
RGCGC↓Y
RGC↓GCY
RGCGCY
RGCGC↓Y
RGCGC↓Y
RGCGCY
RGCGCY
RGC↓GCY
RGCGCY
Hae III
Aca IV
AspT III
Asp742 I
AvrB I
Bal475 I
Bal3006 I
Bce71 I
Bco33 I
Bfi458 I
Bim19 II
Bli I
Blu II
BsaR I
Bse I
BseQ I
Bsh I
BshA I
BshB I
BshC I
BshD I
BshE I
BshF I
BsiA I
BsiD I
BsiH I
BspBR I
BspK I
BspR I
Bsp71 I
Bsp137 I
Bsp211 I
Bsp226 I
Bsp1261 I
BssC I
BstC I
BstJ I
BsuR I
Bsu1076 I
Bsu1114 I
Bte I
Clm I
Clt I
Csp2 I
Dsa II
FinS I
FnuD I
GG↓CC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GG↓CC
GGCC
GGCC
GGCC
GGCC
GG↓CC
GG↓CC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GG↓CC
GG↓CC
GG↓CC
GGCC
GGCC
GG↓CC
GGCC
GGCC
GGCC
GGCC
GGCC
GG↓CC
GGCC
GGCC
GGCC
GGCC
GG↓CC
GGCC
GG↓CC
GGCC
GG↓CC
制限酵素 認識配列*
Hhg I
MfoA I
Mni I
NgoA II
NgoP II
NgoS II
Nla I
Och I
Pai I
Pal I
Pde133 I
PflK I
Pla I
Ple214 I
Ppu I
Psp29 I
Sbv I
Sfa I
Spl III
Sua I
Sul I
TspZN I
TteA I
Ttn I
Uba9 I
Uba54 I
Uba61 I
Uba1097 I
Uba1140 I
Uba1146 I
Uba1147 I
Uba1150 I
Uba1152 I
Uba1153 I
Uba1155 I
Uba1169 I
Uba1174 I
Uba1175 I
Uba1176 I
Uba1178 I
Uba1179 I
Uba1207 I
Uba1208 I
Uba1209 I
Uba1210 I
Uba1214 I
Uba1223 I
Uba1228 I
Uba1230 I
Uba1231 I
Uba1235 I
Uba1288 I
Uba1292 I
Uba1293 I
Uba1319 I
Uba1322 I
Uba1336 I
Uba1377 I
Uba1388 I
Uba1392 I
Uba1395 I
Uba1408 I
Uba1418 I
Uba1422 I
Uba1429 I
Uba1449 I
Uba1450 I
Van91 III
Vha I
Vha1168 I
Vni I
GGCC
GG↓CC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GG↓CC
GG↓CC
GG↓CC
GG↓CC
GGCC
GGCC
GGCC
GG↓CC
GG↓CC
GGCC
GG↓CC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
GGCC
Hae IV (7/13)GAYNNNNNRTC(14/9)
Hga I GACGC(5/10)
HgiA I
Alw21 I
AspH I
Bbv12 I
BsaG I
Bsh45 I
BsiHKA I
BsmP I
Pph3215 I
GWGCW↓C
GWGCW↓C
GWGCW↓C
GWGCW↓C
GWGCWC
GWGCW↓C
GWGCW↓C
GWGCWC
GWGCW↓C
HgiC I
AccB1 I
Ban I
BbvB I
BshN I
BspT107 I
Eco50 I
Eco64 I
Eco168 I
Eco169 I
Eco171 I
Eco173 I
Eco195 I
Esp1 I
Esp6 I
Esp9 I
Esp10 I
Esp11 I
Esp12 I
Esp13 I
Esp14 I
Esp15 I
Esp21 I
Esp22 I
Esp25 I
HgiH I
MspB4 I
Pph14 I
SspM1 III
Uba1127 I
Vsp2246 I
G↓GYRCC
G↓GYRCC
G↓GYRCC
G↓GYRCC
G↓GYRCC
G↓GYRCC
GGYRCC
G↓GYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
G↓GYRCC
G↓GYRCC
GGYRCC
GGYRCC
GGYRCC
GGYRCC
HgiE II ACCNNNNNNGGT
HgiJ II
Ban II
Bsp117 I
Bsp519 I
Bsu1854 I
Bvu I
Cfr48 I
EcoT38 I
EcoT88 I
EcoT93 I
EcoT95 I
Eco24 I
Eco25 I
Eco26 I
Eco35 I
Eco68 I
Eco113 I
Eco180 I
Eco211 I
Eco215 I
Eco216 I
Eco232 I
Eco249 I
Eco262 I
Ese4 I
FriO I
Kox II
PaeH I
Ppu20 I
Psp31 I
SacN I
Uba39 I
Uba57 I
Uba1124 I
Uba1159 I
Uba1206 I
Uba1263 I
Uba1264 I
Uba1307 I
Uba1329 I
Uba1330 I
Uba1357 I
Uba1363 I
Uba1409 I
Uba1421 I
GRGCY↓C
GRGCY↓C
GRGCYC
GRGCY↓C
GRGCY↓C
GRGCY↓C
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCY↓C
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCY↓C
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCY↓C
GRGCY↓C
GRGCY↓C
GRGCYC
GRGCYC
GRGCY↓C
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
GRGCYC
Hha I
AspLE I
Bca I
BstHH I
Cfo I
Csp1470 I
FnuD III
HinGU I
HinP1 I
HinS1 I
HinS2 I
Hin6 I
Hin7 I
HspA I
Mnn IV
SciNI
GCG↓C
GCG↓C
GCGC
GCG↓C
GCG↓C
GCGC
GCG↓C
GCGC
G↓CGC
GCGC
GCGC
G↓CGC
GCGC
G↓CGC
GCGC
G↓CGC
Hind II
Chu II
Hinc II
HinJC I
Hin1160 II
Hin1161 II
Mnn I
GTY↓RAC
GTYRAC
GTY↓RAC
GTY↓RAC
GTYRAC
GTYRAC
GTYRAC
Hind III
Asp52 I
Asp3065 I
Bbr I
Bpe I
BseH I
BstF I
Bst170 II
Cfr32 I
Chu I
Eco VIII
Eco65 I
Eco98 I
Eco188 I
Eco231 I
Hinb III
Hinf II
HinJC II
Hin5 III
Hin173 I
Hin1076 III
Hsu I
Mki I
Ssb I
Uba83 I
Uba1164 II
Uba1219 I
Uba1435 I
A↓AGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
A↓AGCTT
AAGCTT
AAGCTT
AAGCTT
A↓AGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
A↓AGCTT
AAGCTT
A↓AGCTT
AAGCTT
AAGCTT
AAGCTT
AAGCTT
Hinf I
CviB I
CviC I
CviD I
CviE I
CviF I
CviG I
FnuA I
Hha II
HpyA IV
Nca I
NsiH I
Nov II
G↓ANTC
G↓ANTC
GANTC
GANTC
GANTC
GANTC
GANTC
G↓ANTC
G↓ANTC
GANTC
GANTC
GANTC
GANTC
Hin4 I (8/13)GABNNNNNVTC(13/8)
Hin4 II
HpyA V
CCTTC
CCTTC(6/5)
Hpa I
BsaK I
Bse II
BstHP I
KspA I
Ssr I
Uba1408 II
GTT↓AAC
GTTAAC
GTTAAC
GTT↓AAC
GTT↓AAC
GTTAAC
GTTAAC
Hpa II
Asp748 I
Bco27 I
BsaZ I
BshM I
BsiS I
Bsp5 I
Bsp47 I
Bsp48 I
Bsp116 I
Bst40 I
BsuF I
Bsu1192 I
Fin II
Hap II
Hin2 I
Hin5 I
Mni II
Mno I
Msp I
Pde137 I
Pme35 I
Sec II
SfaGU I
Sth134 I
Uba1128 I
Uba1141 I
Uba1267 I
Uba1338 I
Uba1355 I
Uba1439 I
C↓CGG
CCGG
C↓CGG
CCGG
CCGG
C↓CGG
CCGG
CCGG
CCGG
CCGG
C↓CGG
CCGG
CCGG
CCGG
C↓CGG
C↓CGG
CCGG
CCGG
C↓CGG
C↓CGG
C↓CGG
CCGG
CCGG
CCGG
C↓CGG
CCGG
CCGG
CCGG
CCGG
CCGG
CCGG
Hph I
AsuHP I
NgoB VIII
SspD5 I
GGTGA(8/7)
GGTGA(8/7)
GGTGA
GGTGA(8/8)
Hpy99 I CGWCG↓
Hpy178 III
Hpy188 III
TC↓NNGA
TC↓NNGA
Hpy188 I TCN↓GA
Kpn I
Acc65 I
AhaB8 I
Asp718 I
BspJ106 I
Eco149 I
Esp19 I
KpnK14 I
Nmi I
Sau10 I
Sth I
SthA I
SthB I
SthC I
SthD I
SthE I
SthF I
SthG I
SthH I
SthJ I
SthK I
SthL I
SthM I
SthN I
Uba76 I
GGTAC↓C
G↓GTACC
G↓GTACC
G↓GTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
G↓GTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
GGTACC
制限酵素 認識配列*
Uba85 I
Uba86 I
Uba87 I
Uba1201 I
GGTACC
GGTACC
GGTACC
GGTACC
Ksp632 I
Bco5 I
Bco116 I
BseZ I
BsrE I
Bst6 I
Bst158 I
Bsu6 I
Eam1104 I
Ear I
Uba1192 I
Uba1276 I
CTCTTC(1/4)
CTCTTC(1/4)
CTCTTC(1/4)
CTCTTC(1/4)
CTCTTC
CTCTTC(1/4)
CTCTTC
CTCTTC(1/4)
CTCTTC(1/4)
CTCTTC(1/4)
CTCTTC
CTCTTC
Mae I
Bfa I
FspB I
Mja I
MthF I
MthZ I
Rma I
Xsp I
C↓TAG
C↓TAG
C↓TAG
CTAG
CTAG
C↓TAG
C↓TAG
C↓TAG
Mae II
HpyCH4 IV
Tai I
Tsc I
A↓CGT
A↓CGT
ACGT↓
ACGT↓
Mae III ↓GTNAC
Mbo I
AspMD I
Bce243 I
Bme12 I
BsaP I
BscF I
Bsp I
BspA I
BspF I
BspJ I
BspJ64 I
BspKT6 I
Bsp9 I
Bsp18 I
Bsp49 I
Bsp51 I
Bsp52 I
Bsp54 I
Bsp57 I
Bsp58 I
Bsp59 I
Bsp60 I
Bsp61 I
Bsp64 I
Bsp65 I
Bsp66 I
Bsp67 I
Bsp72 I
Bsp74 I
Bsp76 I
Bsp91 I
Bsp105 I
Bsp122 I
Bsp135 I
Bsp136 I
Bsp138 I
Bsp143 I
Bsp147 I
Bsp2095 I
BsrM I
BsrP II
BssG II
BstE III
BstKT I
BstMB I
BstX II
Btc I
Btk II
Btu33 I
Btu34 I
Btu36 I
Btu37 I
Btu39 I
Btu41 I
Cac I
Ccy I
Cha I
Cin1467 I
Cpa I
Cpf I
Csp5 I
Cte1179 I
Cte1180 I
Cty I
CviA I
CviB II
CviH I
Dpn II
FnuA II
FnuC I
FnuE I
Hac I
Kzo9 I
LlaA I
Lsp1109 II
Meu I
MkrA I
Mme II
Mno III
Mos I
MspB I
Msp67 II
Mth I
MthA I
Mth1047 I
Nde II
Nfl I
NflA II
NflB I
Nla II
NlaD I
NmeC I
Nph I
NsiA I
NspA I
Nsu I
Pei9403 I
Pfa I
Pph288 I
Rlu1 I
SalA I
SalH I
Sau3A I
SauC I
SauD I
SauE I
SauF I
SauG I
SauM I
Sau15 I
Sau6782 I
SinM I
Tru II
Uba4 I
Uba59 I
Uba1101 I
Uba1177 I
Uba1182 I
Uba1183 I
Uba1204 I
Uba1259 I
Uba1317 I
Uba1323 I
Uba1336 I
Vha44 I
↓GATC
↓GATC
↓GATC
GATC
GATC
GATC
GATC
↓GATC
↓GATC
↓GATC
GATC
GAT↓C
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
↓GATC
GATC
GATC
GATC
GATC
↓GATC
GATC
GATC
GATC
GATC
↓GATC
GATC
↓GATC
GATC
GATC
GATC
GATC
GAT↓C
↓GATC
GATC
GATC
↓GATC
GATC
GATC
GATC
GATC
GATC
GATC
↓GATC
↓GATC
GATC↓
GATC
GATC
↓GATC
GATC
GATC
GATC
GATC
↓GATC
GATC
GATC
↓GATC
GATC
↓GATC
↓GATC
↓GATC
↓GATC
↓GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
↓GATC
GATC
GATC
GATC
↓GATC
GATC
↓GATC
↓GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
↓GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
GATC
Mbo II
Ncu I
Tce I
GAAGA(8/7)
GAAGA
GAAGA
Mcr I
BsaO I
Bsh1285 I
BsiE I
BstMC I
Uba1303 I
CGRY↓CG
CGRY↓CG
CGRY↓CG
CGRY↓CG
CGRY↓CG
CGRYCG
Mfe I
Mun I
C↓AATTG
C↓AATTG
Mja IV
Hpy8 I
GTNNAC
GTNNAC
Mlu I
Ape I
Uba6 I
A↓CGCGT
ACGCGT
ACGCGT
Mme I TCCRAC(20/18)
Mnl I CCTC(7/6)
Mse I
RspRS II
SaqA I
Tru1 I
Tru9 I
T↓TAA
T↓TAA
T↓TAA
T↓TAA
T↓TAA
Msl I
SmiM I
CAYNN↓NNRTG
CAYNN↓NNRTG
Mst I
Aca III
Acc16 I
Aos I
Avi II
Bco6 I
BsaT I
Clc II
Cli II
Fdi II
Fsp I
GspA II
Nsb I
NspH III
NspL I
NspM I
Pam I
TGC↓GCA
TGCGCA
TGC↓GCA
TGC↓GCA
TGC↓GCA
TGCGCA
TGCGCA
TGCGCA
TGCGCA
TGC↓GCA
TGC↓GCA
TGCGCA
TGC↓GCA
TGCGCA
TGCGCA
TGCGCA
TGC↓GCA
Mwo I
Bce1247 I
BspW I
HpyF10 VI
GCNNNNN↓NNGC
GCNNNNNNNGC
GCNNNNN↓NNGC
GCNNNNNN↓NGC
Nae I
Afa24R I
Ame II
AniM I
ApeA I
Apr I
Eco56 I
Esp5 I
MauA I
Mis I
Mlu9273 II
MroN I
NasW I
Nba I
Nbr I
NgoA IV
NgoM IV
Nmu I
NmuF I
NspW I
NtaS II
Pgl I
Psp61 I
Rlu I
SacA I
SalC I
Sao I
SauA I
SauBMK I
SauHP I
SauLP I
SauN I
SauS I
Ska I
Slu1777 I
Smo40529 I
SspC I
Uba1122 I
GCC↓GGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
G↓CCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
G↓CCGGC
GCCGGC
GCCGGC
GCCGGC
G↓CCGGC
G↓CCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCCGGC
GCC↓GGC
GCC↓GGC
GCC↓GGC
GCC↓GGC
GCC↓GGC
GCCGGC
GCC↓GGC
GCCGGC
GCC↓GGC
GCCGGC
Nar I
Bbe I
BbeA I
BinS II
Eco78 I
Ege I
Ehe I
Kas I
McaA I
Mch I
Mly113 I
Msa I
Nam I
Nda I
Nun II
Pmn I
Sfo I
SseA I
GG↓CGCC
GGCGC↓C
GGCGCC
GGCGCC
GGC↓GCC
GGC↓GCC
GGC↓GCC
G↓GCGCC
GGCGCC
GG↓CGCC
GG↓CGCC
GGCGCC
GGCGCC
GG↓CGCC
GG↓CGCC
GGC↓GCC
GGCGCC
GG↓CGCC
Nco I
Ate I
Bsp19 I
NspSA III
C↓CATGG
CCATGG
C↓CATGG
CCATGG
Nde I
FauND I
CA↓TATG
CA↓TATG
Nhe I
Ace II
Bmt I
PstNH I
G↓CTAGC
GCTAG↓C
GCTAG↓C
G↓CTAGC
Nla III
CviA II
Fat I
Hin1 II
Hin8 II
HpyCH4 I
Hsp92 II
CATG↓
C↓ATG
↓CATG
CATG
CATG
CAT↓G
CATG↓
Nla IV
AspN I
Bcr I
BsaE I
BscB I
BspL I
Bsp29 I
Bss I
PspN4 I
Rlu3 I
Uba1305 I
Uba1445 I
GGN↓NCC
GGN↓NCC
GGNNCC
GGNNCC
GGN↓NCC
GGN↓NCC
GGNNCC
GGNNCC
GGN↓NCC
GGNNCC
GGNNCC
GGNNCC
Not I
CciN I
GC↓GGCCGC
GC↓GGCCGC
Nru I
Ama I
Bsp68 I
MluB2 I
Mlu9273 I
PspD I
SalD I
Sbo13 I
Sna3286 I
TCG↓CGA
TCGCGA
TCG↓CGA
TCG↓CGA
TCGCGA
TCGCGA
TCGCGA
TCG↓CGA
TCGCGA
制限酵素 認識配列*
Spo I
Uba1117 I
Uba1386 I
TCG↓CGA
TCGCGA
TCGCGA
Nsp I
BstNS I
Lsp1270 I
NspH I
Xce I
RCATG↓Y
RCATG↓Y
RCATGY
RCATG↓Y
RCATG↓Y
NspB II
MspA1 I
CMG↓CKG
CMG↓CKG
Oli I
Ale I
CACNN↓NNGTG
CACNN↓NNGTG
Pac I TTAAT↓TAA
Pas I CC↓CWGGG
PflM I
AccB7 I
Acp II
Asp10H II
Esp1396 I
Van91 I
CCANNNN↓NTGG
CCANNNN↓NTGG
CCANNNN↓NTGG
CCANNNN↓NTGG
CCANNNN↓NTGG
CCANNNN↓NTGG
Pfl1108 I TCGTAG
Pfo I T↓CCNGGA
Ple I
BsmE I
Mly I
PpsI
SchI
GAGTC(4/5)
GAGTC
GAGTC(5/5)
GAGTC(4/5)
GAGTC(5/5)
PmaC I
BbrP I
BcoA I
Bsp87 I
Eco72 I
Pgl34 I
Pml I
PspB I
Psp38 I
CAC↓GTG
CAC↓GTG
CAC↓GTG
CACGTG
CAC↓GTG
CACGTG
CAC↓GTG
CACGTG
CACGTG
Pme I
MssI
GTTT↓AAAC
GTTT↓AAAC
Ppi I (7/12)GAACNNNNNCTC(13/8)
PpuM I
Mlu1106 I
Pfl27 I
PspPP I
Psp5 II
RG↓GWCCY
RGGWCCY
RG↓GWCCY
RG↓GWCCY
RG↓GWCCY
PshA I
Box I
BstPA I
GACNN↓NNGTC
GACNN↓NNGTC
GACNN↓NNGTC
PsiI TTA↓TAA
PspX I VC↓TCGAGB
Psr I (7/12)GAACNNNNNNTAC(12/7)
Pst I
Ain I
AliAJ I
Ali2882 I
Api I
AspT I
Asp36 I
Asp708 I
Asp713 I
Bbi I
Bce170 I
BmeB I
BsaN II
BsaQ I
BscD I
BspB I
Bsp17 I
Bsp43 I
Bsp63 I
Bsp78 I
Bsp81 I
Bsp93 I
Bsp107 I
Bsp108 I
Bsp268 I
BsuB I
Cau III
Cfl I
CfrA4 I
Cfu II
Clc I
Cst I
EaeP I
Ecl77 I
Ecl133 I
Ecl593 I
Eco48 I
Eco49 I
Eco83 I
Eco133 I
Eco141 I
Eco161 I
Eco167 I
Eco260 I
Eco261 I
Esp5 II
Esp141 I
Gse II
Hal II
Kpn12 I
Mau I
MhaA I
Miz I
Mkr I
Nas I
Ngb I
Noc I
PaeP I
Pae8 I
Pae9 I
Pae14 I
Pae15 I
Pae22 I
Pae24 I
Pae25 I
Pae26 I
Pae39 I
Pae40 I
Pae41 I
Pfl37 I
Pma I
Pma44 I
Pmy I
Pph2059 I
PspS I
Psp28 I
Psp46 I
SalP I
Sal13 I
ScoA I
Sfl I
Ska II
SprL I
UbaHKB I
Uba46 I
Uba71 I
Uba72 I
Uba1112 I
Uba1115 I
Uba1116 I
Uba1119 I
Uba1123 I
Uba1149 I
Uba1184 I
Uba1186 I
Uba1211 I
Uba1212 I
Uba1213 I
Uba1215 I
Uba1216 I
Uba1225 I
Uba1232 I
Uba1256 I
Uba1262 I
Uba1287 I
Uba1294 II
Uba1296 I
Uba1328 I
Uba1337 I
Uba1399 I
Uba1411 I
Uba1417 I
Xma II
Xor I
Xph I
Yen I
YenA I
YenB I
YenC I
YenD I
YenE I
CTGCA↓G
CTGCAG
CTGCA↓G
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCA↓G
CTGCA↓G
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCA↓G
CTGCAG
CTGCAG
CTGCAG
CTGCAG
CTGCAG
Pvu I
Afa16R I
Afa22M I
Bma I
BmaA I
BmaB I
BmaC I
BmaD I
BspC I
Cas2 I
Cfr51 I
Drd III
EclJ I
ErhB9 I
Kpl79 I
Nbl I
Ple19 I
Psu161 I
Pvu84 I
Rsh I
Rsp I
SmaA III
SplA III
Uba1129 I
Uba1139 I
Xgl3216 I
Xgl3217 I
Xgl3218 I
Xgl3219 I
Xgl3220 I
Xml I
XmlA I
Xni I
Xor II
CGAT↓CG
CGA↓TCG
CGA↓TCG
CGA↓TCG
CGATCG
CGATCG
CGATCG
CGATCG
CGAT↓CG
CGATCG
CGATCG
CGATCG
CGATCG
CGAT↓CG
CGATCG
CGAT↓CG
CGAT↓CG
CGAT↓CG
CGATCG
CGAT↓CG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGATCG
CGAT↓CG
Pvu II
Bav I
BavA I
BavB I
BspO4 I
Cfr6 I
Dma I
Gsp I
Mzi I
NmeR I
Psp3 I
Psp5 I
PvuHKu I
Pvu84 II
Sba I
SciA II
SmaA IV
Sol3335 I
SplA IV
Uba1227 I
Uba1245 I
CAG↓CTG
CAG↓CTG
CAG↓CTG
CAG↓CTG
CAG↓CTG
CAG↓CTG
CAG↓CTG
CAGCTG
CAGCTG
CAGCTG
CAGCTG
CAGCTG
CAGCTG
CAG↓CTG
CAGCTG
CAGCTG
CAGCTG
CAGCTG
CAGCTG
CAGCTG
CAGCTG
RleA I CCCACA(12/9)
Rsa I
Afa I
Asp 16H I
Asp 17H I
Asp 18H I
Asp 29H I
Csp 6 I
CviQ I
CviR II
GT↓AC
GT↓AC
GTAC
GTAC
GTAC
GTAC
G↓TAC
G↓TAC
G↓TAC
Rsr II
Cpo I
Csp I
Rsr2 I
CG↓GWCCG
CG↓GWCCG
CG↓GWCCG
CG↓GWCCG
Sac I
Ecl136 II
Ecl137 II
EcoICR I
Mxa I
NasS I
Pfl18 I
Psp124B I
Sco I
Sst I
GAGCT↓C
GAG↓CTC
GAGCTC
GAG↓CTC
GAG↓CTC
GAGCTC
GAGCTC
GAGCT↓C
GAGCTC
GAGCT↓C
Sac II
Aos III
Asp32H I
Bac I
Bac465 I
Bsp12 I
Cfr37 I
Cfr41 I
Cfr42 I
Cfr43 I
Cfr45 II
Csc I
Cte1 I
DrdA I
DrdB I
DrdC I
DrdE I
DrdF I
Dsp1 I
Eae46 I
Ecc I
Ecl1 I
Ecl28 I
Ecl37 I
Eco29k I
Eco55 I
Eco92 I
Eco96 I
Eco99 I
Eco100 I
Eco104 I
Eco134 I
Eco135 I
Eco151 I
Eco158 I
Eco182 I
Eco196 I
Eco208 I
Ese3 I
Ese6 I
Fsc I
Gal I
Gce I
GceGL I
Kpn19 I
Kpn378 I
CCGC↓GG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGC↓GG
CCGC↓GG
CCGC↓GG
CCGC↓GG
CCGCGG
制限酵素 認識配列*
Ksp I
Mra I
NgoA III
NgoD I
NgoP III
NlaD III
NlaS I
PaeA I
PaeQ I
Pae7 I
Pae17 I
Pae36 I
Pae42 I
Pae43 I
Pae44 I
Plf1108 II
Saa I
Sab I
Sak I
Sbo I
Sfr I
Sfr303 I
Sfr382 I
SgrB I
Shy I
Spu I
Sse II
Ssp1725 I
Sst II
Sta I
Tgl I
Tto I
UbaHKA I
Uba66 I
Uba77 I
Uba90 I
Uba1093 I
Uba1095 I
Uba1111 I
Uba1113 I
Uba1126 I
Uba1187 I
Uba1229 I
Uba1234 I
Uba1244 I
Uba1306 I
Uba1364 I
Uba1369 I
CCGC↓GG
CCGCGG
CCGC↓GG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGC↓GG
CCGCGG
CCGC↓GG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGC↓GG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
CCGCGG
Sal I
Acs1371 I
Acs1372 I
Acs1373 I
Acs1421 I
Acs1422 I
HgiC III
HgiD II
Koy I
Nop I
Psp32 I
Psp33 I
Psp89 I
RflF I
Rhe I
Rhp I
Rrh I
Rrh4273 I
Rro I
Rtr I
Uba89 I
Xam I
Xci I
G↓TCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
G↓TCGAC
G↓TCGAC
GTCGAC
G↓TCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
GTCGAC
G↓TCGAC
GTCGAC
GTCGAC
G↓TCGAC
SanD I
Sse1825 I
GG↓GWCCC
GG↓GWCCC
Sap I
BspQ I
VpaK32 I
GCTCTTC(1/4)
GCTCTTC
GCTCTTC(1/4)
Sau I
Aoc I
Axy I
BliHK I
Bse21 I
Bst29 I
Bst30 I
Bsu36 I
Cvn I
EciC I
Eco76 I
Eco81 I
Eco115 I
Eco118 I
Lmu60 I
Mst II
OxaN I
SauH I
Sec III
SshA I
Uba1184 I
Uba1294 I
Uba1332 I
Uba1333 I
CC↓TNAGG
CC↓TNAGG
CC↓TNAGG
CC↓TNAGG
CC↓TNAGG
CCTNAGG
CCTNAGG
CC↓TNAGG
CC↓TNAGG
CCTNAGG
CCTNAGG
CC↓TNAGG
CCTNAGG
CCTNAGG
CC↓TNAGG
CC↓TNAGG
CC↓TNAGG
CCTNAGG
CCTNAGG
CC↓TNAGG
CCTNAGG
CCTNAGG
CCTNAGG
CCTNAGG
Sca I
Acc113 I
Afl IV
Asp763 I
BshH I
BsoS I
BstM I
Dpa I
Eco255 I
Pin I
RflF II
Uba1094 I
Uba1158 I
AGT↓ACT
AGT↓ACT
AGTACT
AGTACT
AGTACT
AGTACT
AGTACT
AGT↓ACT
AGT↓ACT
AGTACT
AGT↓ACT
AGTACT
AGTACT
ScrF I
Bme1390 I
BsaC I
Bso I
Bsp53 I
Bsp73 I
BssK I
Dsa V
Eco43 I
Eco51 II
Eco80 I
Eco85 I
Eco93 I
Eco153 I
Eco200 I
MspR9 I
Msp67 I
Nla X
Sso II
Uba17 I
Uba1391 I
CC↓NGG
CC↓NGG
CCNGG
CCNGG
CCNGG
CCNGG
↓CCNGG
↓CCNGG
CCNGG
CCNGG
CCNGG
CCNGG
CCNGG
CCNGG
CCNGG
CC↓NGG
CC↓NGG
CCNGG
↓CCNGG
CCNGG
CCNGG
Sdu I
Aoc II
Bka1125 I
Bmy I
BsoC I
BspLS2 I
Bsp1286 I
Nsp II
Nsp7524 II
Uba1362 I
GDGCH↓C
GDGCH↓C
GDGCHC
GDGCH↓C
GDGCHC
GDGCH↓C
GDGCH↓C
GDGCH↓C
GDGCH↓C
GDGCHC
Sec I
BsaJ I
BseD I
BssEC I
Uba1442 I
C↓CNNGG
C↓CNNGG
C↓CNNGG
C↓CNNGG
CCNNGG
SexA I
Mab I
A↓CCWGGT
A↓CCWGGT
SfaN I
BscA I
BsmN I
Lwe I
GCATC(5/9)
GCATC(4/6)
GCATC
GCATC(5/9)
Sfe I
Bco 163 I
BdiS I
Bfm I
Bpc I
BstSF I
LlaB I
Sfc I
C↓TRYAG
CTRYAG
C↓TRYAG
C↓TRYAG
C↓TRYAG
C↓TRYAG
C↓TRYAG
C↓TRYAG
Sfi I
Sdi I
GGCCNNNN↓NGGCC
GGCCNNNN↓NGGCC
Sgf I
AsiS I
Rga I
GCGAT↓CGC
GCGAT↓CGC
GCGAT↓CGC
SgrA I CR↓CCGGYG
Sim I
BtsP I
Sai I
GGGTC(-3/0)
GGGTC
GGGTC
Sma I
Ahy I
CfrJ4 I
Cfr9 I
EaeA I
EclR I
PaeB I
PspA I
PspAL I
Uba1220 I
Uba1393 I
UthS I
Xcy I
Xma I
XmaC I
CCC↓GGG
C↓CCGGG
CCC↓GGG
C↓CCGGG
C↓CCGGG
CCCGGG
CCC↓GGG
C↓CCGGG
CCC↓GGG
CCCGGG
CCCGGG
CC↓CGGG
C↓CCGGG
C↓CCGGG
C↓CCGGG
Sml I
Smo I
C↓TYRAG
C↓TYRAG
Sna I
BspM90 I
BssNA I
BstBS I
BstZ17 I
Bst1107 I
Xca I
GTATAC
GTA↓TAC
GTA↓TAC
GTA↓TAC
GTATAC
GTA↓TAC
GTA↓TAC
SnaB I
BstSN I
EciA I
Eco105 I
Eco158 II
SspJ I
SspM1 I
SspM2 I
Uba1240 I
TAC↓GTA
TAC↓GTA
TACGTA
TAC↓GTA
TACGTA
TACGTA
TACGTA
TACGTA
TACGTA
Spe I
AclN I
Ahl I
Bcu I
A↓CTAGT
A↓CTAGT
A↓CTAGT
A↓CTAGT
Sph I
Asp5H I
Bbu I
Bsp121 I
Pae I
PaeC I
SpaX I
Uba1162 I
Uba1226 I
GCATG↓C
GCATGC
GCATG↓C
GCATGC
GCATG↓C
GCATGC
GCATGC
GCATGC
GCATGC
Spl I
BpuB5 I
BsiW I
Pfl23 II
PfuN I
PpuA I
PspL I
SmaA I
SplA I
SspK I
Sun I
C↓GTACG
C↓GTACG
C↓GTACG
C↓GTACG
CGTACG
C↓GTACG
C↓GTACG
CGTACG
CGTACG
CGTACG
C↓GTACG
Srf I GCCC↓GGGC
Sse232 I
Mre I
CG↓CCGGCG
CG↓CCGGCG
Sse8387 I
Sbf I
Sda I
CCTGCA↓GG
CCTGCA↓GG
CCTGCA↓GG
Sse8647 I AG↓GWCCT
Ssp I AAT↓ATT
Sth132 I CCCG(4/8)
Stu I
Aat I
Asp78 I
Chy I
Eco147 I
Gdi I
GobA I
NtaS I
Pce I
Pme55 I
Ppu13 I
Sar I
SseB I
Ssv I
Ste I
Uba40 I
Uba1170 I
Uba1180 I
Uba1217 I
Uba1239 I
Uba1371 I
Uba1403 I
Uba1419 I
AGG↓CCT
AGG↓CCT
AGGCCT
AGGCCT
AGG↓CCT
AGG↓CCT
AGGCCT
AGGCCT
AGG↓CCT
AGG↓CCT
AGGCCT
AGG↓CCT
AGG↓CCT
AGGCCT
AGGCCT
AGGCCT
AGGCCT
AGGCCT
AGGCCT
AGGCCT
AGGCCT
AGGCCT
AGGCCT
Sty I
BsmS I
BssT1 I
Bst224 I
EcoT14 I
EcoT104 I
Eco130 I
Eco208 II
Erh I
ErhB9 II
SblA I
SblB I
SblC I
Uba1311 I
C↓CWWGG
CCWWGG
C↓CWWGG
CCWWGG
C↓CWWGG
CCWWGG
C↓CWWGG
CCWWGG
C↓CWWGG
C↓CWWGG
CCWWGG
CCWWGG
CCWWGG
CCWWGG
Swa I
Smi I
ATTT↓AAAT
ATTT↓AAAT
Taq I
CviS III
EsaBC3 I
Tfl I
TthHB8 I
T↓CGA
TCGA
TC↓GA
TCGA
T↓CGA
Taq II GACCGA(11/9)
CACCCA(11/9)
Tat I WGTACW
制限酵素 認識配列*
Tau I
BspU I
GCSG↓C
GCSG↓C
Tfi I
Pfe I
G↓AWTC
GAWTC
Tse I G↓CWGC
Tso I TARCCA
TspDT I ATGAA(11/9)
TspE I
MluC I
Sse9 I
TasI
Tsp509 I
↓AATT
AATT
↓AATT
↓AATT
↓AATT
TspGW I ACGGA(11/9)
TspR I CASTGNN↓
Tsp4C I
Bst4C I
HpyCH4 III
TaaI
ACN↓GT
ACN↓GT
ACN↓GT
ACN↓GT
Tsp45 I
NmuC I
↓GTSAC
↓GTSAC
Tth111 I
Asp I
Ats I
Fsu I
Nta I
PflF I
PsyI
SmaA II
Spl II
SplA II
Tel I
Tsp I
Tte I
Ttr I
GACN↓NNGTC
GACN↓NNGTC
GACN↓NNGTC
GACNNNGTC
GACNNNGTC
GACNNNGTC
GACN↓NNGTC
GACNNNGTC
GACNNNGTC
GACNNNGTC
GACN↓NNGTC
GACNNNGTC
GACNNNGTC
GACNNNGTC
Tth111 II CAARCA(11/9)
UbaD I GAACNNNNNNTCC
UbaE I CACCTGC
UbaF2 I GAAAYNNNNNRTG
UbaF3 I CACNNNNNNTCC
UbaF4 I GAANNNNNNNTTGG
UbaF5 I CTGATG
UbaF6 I GCGAC
UbaG I CACNNNNGTG
UbaH I GCANNNNNNTGC
UbaP I CGAACG
UbaT I TGANNNNNNTCA
Vsp I
Ase I
Asn I
PshB I
Srl17D I
AT↓TAAT
AT↓TAAT
AT↓TAAT
AT↓TAAT
ATTAAT
Xba I
BspLU11 II
BsrX I
Msp23 I
T↓CTAGA
TCTAGA
TCTAGA
TCTAGA
Xcm I CCANNNNN↓NNNNTGG
Xho I
Abr I
Asp15 I
Asp47 I
Asp703 I
Bad I
Bbf I
Bbi III
Blu I
Bsp92 I
Bsp129 I
Bsp139 I
Bsp140 I
Bsp141 I
Bsp142 I
BssH I
BstH I
BstL I
BstV I
BsuM I
Bth I
Ccr I
Cja I
Dde II
DraD I
Eae2 I
Mav I
Mca I
Mec I
Mha I
MlaA I
Mpu I
Mrh I
MscA I
Msi I
Msp23 II
Oco I
PaeR7 I
Pan I
PflN I
PflW I
Pfl67 I
Psp4 I
Sal1974 I
Sau3239 I
Sbi68 I
Sca1827 I
Sci I
SciB I
Sci1831 I
Scu I
Sex I
Sfr274 I
Sfu1762 I
Sga I
Sgo I
Sgr1841 I
Shy1766 I
Sla I
Slu I
Sol10179 I
Spa I
Sph1719 I
Ssp4 I
StaA I
Sve194 I
Tli I
Uba1130 I
Uba1148 I
Uba1154 I
Uba1166 I
Uba1237 I
Uba1248 I
Uba1271 I
Uba1298 I
Uba1335 I
Uba1397 I
Uba1448 I
Xpa I
C↓TCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CT↓CGAG
CTCGAG
CT↓CGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
C↓TCGAG
CTCGAG
CTC↓GAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
CTCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
CTCGAG
C↓TCGAG
Xho II
Ait II
AitA I
Asp1H I
Asp6H I
Asp8H I
Asp14H I
Asp17 I
Asp21H I
Asp22 I
BstX2 I
BstY I
Dsa III
Mfl I
Psu I
Tru201 I
Uba1432 I
R↓GATCY
RGATCY
RGATCY
RGATCY
RGATCY
RGATCY
RGATCY
RGATCY
RGATCY
RGATCY
R↓GATCY
R↓GATCY
R↓GATCY
R↓GATCY
R↓GATCY
R↓GATCY
RGATCY
Xma III
Aaa I
BseX3 I
BsoD I
BstZ I
Eag I
EclX I
Eco52 I
C↓GGCCG
C↓GGCCG
C↓GGCCG
CGGCCG
C↓GGCCG
C↓GGCCG
C↓GGCCG
C↓GGCCG
Xmn I
Asp700 I
BbvA I
MroX I
Pdm I
Syn II
GAANN↓NNTTC
GAANN↓NNTTC
GAANN↓NNTTC
GAANN↓NNTTC
GAANN↓NNTTC
GAANNNNTTC

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