


| Tissue | Tested Cell Lines | Cell Lines Origin | Remarks |
|---|---|---|---|
| Brain | BV2 | immortalized murine primary microglial cells | Coat with Fibronectin: 1 μg/cm2 |
| Breast | MCF-7 | pleural effusion of metastatic human breast adenocarcinoma | Coat with Fibronectin: 1 μg/cm2 |
| Colon | HT-29 | human colon adenocarcinoma | Coat with Vitronectin: 0.5 μg/cm2 |
| Connective tissue | HT1080 | human fibrosarcoma | Coat with Fibronectin: 1 μg/cm2 |
| Liver | HepG2 | hepatocellular carcinoma of the human liver | Coat with Vitronectin: 0.5 μg/cm2 |
| Lung | A-549 | human lung carcinoma | Coat with Vitronectin: 0.5 μg/cm2 |
| Prostate | LNCaP | lymph node metastasis of human prostate adenocarcinoma | 3D Tumorsphere Medium XF(製品コード C-28070)での3次元培養を推奨 |
| Primary Cancer Culture System | 3D Tumorsphere Medium XF | Cancer Cell Line Medium XF | ||
| Main application | Main application | Primary cancer cell isolation/maintenance of heterogeneity | Extended 3D tumorsphere culture of cancer cell lines/enrichment of CSCs | Adherent long-term expansion of cancer cell lines |
| Culture pattern | 2D/3D | 3D | 2D | |
| Formulation | Defined formulation, animal-component free | Serum-free, xeno-free | Serum-free, xeno-free | |
| Additional supplementation required | No | No | No | |
| Plasticware | TC-treated | Suspension culture | TC-treated | |
| Culture surface treatment required | NCCD-reagent (incl.) | No | Human Fibronectin | |
| Product Application | Slective isolation of malignant cells from primary tumors | ✔ | × | × |
| Slective isolation of malignant cells from PDX | ✔ | × | × | |
| Limited stroma support | ✔ | × | × | |
| Prevention of stroma overgrowth | ✔ | × | × | |
| Enrichment of malignant cells from established culture | ✔ | × | × | |
| Depletion of non-cancerous cells from established cultures | ✔ | × | × | |
| Serial 3D tumorsphere culture of established cancer cell lines | × | ✔ | × | |
| Serial 3D mammosphere culture of established cancer cell lines | × | ✔ | × | |
| Serial 3D neurosphere culture of established cancer cell lines | × | ✔ | × | |
| Mammosphere culture(primary neural stem cells) | × | ✔ | × | |
| Neurosphere culture(primary neural stem cells) | × | ✔ | × | |
| Adherent culture of established cancer cell lines | × | × | ✔ | |
| Establish cell lines from primary cancer cells isolated with PCCS | × | ✔ | ✔ |