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| 制限酵素 | 認識配列* | 切 断 部 位 数 | ||||||||
| pKF3 | pUC19 | pUC119 | pBR322 | ColE 1 | M13mp18 | λ | Ad2 | |||
| Aat II | GACGTC | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 10 | 3 |
| Acc I | GTMKAC | 2 | 1 | 1 | 2 | 2 | 2 | 1 | 9 | 17 |
| Acc II | CGCG | 7 | 10 | 14 | 23 | 14 | 24 | 18 | 157 | 303 |
| Acc III | TCCGGA | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 24 | 8 |
| Ace III | CAGCTC(7/11) | 3 | 3 | 4 | 5 | 1 | 9 | 2 | 29 | 29 |
| Aci I | CCGC(-3/-1) | 22 | 34 | 39 | 67 | 36 | 53 | 42 | 516 | 582 |
| Afa I | GTAC | 5 | 3 | 4 | 3 | 11 | 19 | 19 | 113 | 83 |
| Afl II | CTTAAG | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 4 |
| Afl III | ACRYGT | 2 | 1 | 1 | 1 | 2 | 6 | 3 | 20 | 25 |
| Age I | ACCGGT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 5 |
| Alu I | AGCT | 14 | 15 | 17 | 17 | 24 | 44 | 27 | 143 | 158 |
| AlwN I | CAGNNNCTG | 1 | 1 | 1 | 1 | 0 | 7 | 1 | 41 | 25 |
| Aor13H I | TCCGGA | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 24 | 8 |
| Aor51H I | AGCGCT | 1 | 0 | 0 | 4 | 0 | 0 | 2 | 2 | 13 |
| Apa I | GGGCCC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 12 |
| ApaB I | GCANNNNNTGC | 1 | 1 | 0 | 2 | 2 | 1 | 0 | 33 | 20 |
| ApaL I | GTGCAC | 1 | 3 | 3 | 3 | 1 | 1 | 0 | 4 | 7 |
| Apo I | RAATTY | 3 | 1 | 3 | 1 | 7 | 8 | 11 | 58 | 29 |
| Asc I | GGCGCGCC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
| Ava I | CYCGRG | 2 | 1 | 2 | 1 | 1 | 1 | 2 | 8 | 40 |
| Ava II | GGWCC | 1 | 2 | 2 | 8 | 1 | 4 | 1 | 35 | 73 |
| Avi II | TGCGCA | 1 | 2 | 2 | 4 | 1 | 0 | 1 | 15 | 17 |
| Bae I | (10/15)ACNNNNGTAYC(12/7) | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 10 | 5 |
| Bal I | TGGCCA | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 18 | 17 |
| BamH I | GGATCC | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 5 | 3 |
| Ban II | GRGCYC | 1 | 1 | 2 | 2 | 0 | 0 | 2 | 7 | 57 |
| Bbe I | GGCGCC | 0 | 1 | 1 | 4 | 2 | 0 | 1 | 1 | 20 |
| Bbv I | GCAGC(8/12) | 7 | 12 | 13 | 21 | 14 | 34 | 10 | 199 | 179 |
| Bbv II | GAAGAC(2/6) | 0 | 0 | 0 | 3 | 3 | 3 | 0 | 24 | 27 |
| Bcc I | CCATC | 1 | 3 | 5 | 9 | 12 | 13 | 14 | 145 | 62 |
| Bcef I | ACGGC(12/13) | 3 | 2 | 3 | 3 | 11 | 9 | 7 | 115 | 80 |
| Bce83 I | CTTGAG(16/14) | 3 | 4 | 4 | 6 | 2 | 7 | 5 | 13 | 19 |
| Bcg I | (10/12)GCANNNNNNTCG(12/10) | 0 | 1 | 1 | 3 | 2 | 2 | 0 | 28 | 10 |
| Bci VI | GGATAC | 1 | 2 | 2 | 2 | 4 | 4 | 0 | 26 | 9 |
| Bcn I | CCSGG | 5 | 7 | 7 | 10 | 1 | 18 | 4 | 114 | 97 |
| Bet I | WCCGGW | 4 | 3 | 3 | 5 | 3 | 11 | 6 | 81 | 28 |
| Bfi I | ACTGGG(4/11) | 1 | 2 | 2 | 5 | 0 | 3 | 1 | 4 | 22 |
| Bgl I | GCCNNNNNGGC | 0 | 2 | 2 | 3 | 0 | 0 | 1 | 29 | 20 |
| Bgl II | AGATCT | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 11 |
| Bin I | GGATC(4/5) | 6 | 10 | 10 | 12 | 0 | 11 | 4 | 58 | 35 |
| Bln I | CCTAGG | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
| BmeT110 I | CYCGRG | 2 | 1 | 2 | 1 | 1 | 1 | 2 | 8 | 40 |
| Bmg I | GKGCCC | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 50 |
| BmgT120 I | GGNCC | 3 | 6 | 7 | 15 | 2 | 8 | 4 | 74 | 164 |
| Bpl I | GAGNNNNNCTC | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 12 |
| Bpu10 I | CCTNAGC(-5/-2) | 1 | 0 | 0 | 1 | 7 | 3 | 4 | 19 | 23 |
| Bpu1102 I | GCTNAGC | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 8 |
| BsaA I | YACGTR | 2 | 0 | 1 | 1 | 2 | 6 | 5 | 14 | 22 |
| BsaB I | GATNNNNATC | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 21 | 2 |
| BsaX I | ACNNNNNCTCC | 1 | 1 | 2 | 0 | 0 | 2 | 4 | 19 | 29 |
| Bsb I | CAACAC | 0 | 1 | 2 | 2 | 0 | 1 | 2 | 18 | 12 |
| BscG I | CCCGT | 4 | 6 | 7 | 12 | 2 | 23 | 8 | 104 | 71 |
| BseM II | CTSAG | 3 | 5 | 5 | 7 | 10 | 10 | 23 | 79 | 75 |
| BseR I | GAGGAG(10/8) | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 19 | 63 |
| Bsg I | GTGCAG(16/14) | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 41 | 34 |
| Bsi I | CTCGTG(-5/-1) | 1 | 3 | 3 | 3 | 2 | 2 | 0 | 8 | 11 |
| BsiY I | CCNNNNNNNGG | 9 | 6 | 9 | 20 | 19 | 21 | 17 | 176 | 216 |
| Bsm I | GAATGC(1/-1) | 3 | 0 | 0 | 1 | 4 | 2 | 1 | 46 | 10 |
| BsmA I | GTCTC(1/5) | 2 | 4 | 4 | 3 | 4 | 10 | 5 | 37 | 60 |
| BspG I | CTGGAC | 0 | 0 | 0 | 3 | 2 | 3 | 1 | 17 | 31 |
| BspH I | TCATGA | 0 | 3 | 3 | 4 | 3 | 2 | 1 | 8 | 3 |
| BspLU11 I | ACATGT | 1 | 1 | 1 | 1 | 0 | 2 | 3 | 2 | 9 |
| BspM I | ACCTGC(4/8) | 1 | 1 | 1 | 1 | 3 | 4 | 3 | 41 | 39 |
| Bsp1286 I | GDGCHC | 3 | 5 | 6 | 10 | 3 | 3 | 5 | 38 | 105 |
| Bsp1407 I | TGTACA | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 5 |
| Bsp24 I | (8/13)GACNNNNNNTGG(12/7) | 2 | 3 | 3 | 4 | 1 | 2 | 1 | 18 | 18 |
| BspT104 I | TTCGAA | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 7 | 1 |
| BspT107 I | GGYRCC | 2 | 4 | 5 | 9 | 3 | 2 | 7 | 25 | 57 |
| Bsr I | ACTGG(1/-1) | 6 | 11 | 12 | 19 | 9 | 23 | 18 | 110 | 86 |
| BsrB I | GAGCGG(-3/-3) | 1 | 3 | 4 | 2 | 1 | 3 | 4 | 17 | 28 |
| BsrD I | GCAATG(2/0) | 1 | 2 | 2 | 2 | 4 | 3 | 3 | 44 | 14 |
| BssH II | GCGCGC | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 6 | 52 |
| Bst1107 I | GTATAC | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 3 |
| BstP I | GGTNACC | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 13 | 10 |
| BstX I | CCANNNNNNTGG | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 13 | 10 |
| Cac8 I | GCNNGC | 10 | 14 | 17 | 31 | 19 | 23 | 28 | 237 | 285 |
| CfrA I | GCANNNNNNNNGTGG | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 10 | 8 |
| Cfr10 I | RCCGGY | 0 | 1 | 2 | 7 | 0 | 5 | 1 | 61 | 40 |
| Cfr13 I | GGNCC | 3 | 6 | 7 | 15 | 2 | 8 | 4 | 74 | 164 |
| Cje I | (8/14)CCANNNNNNGT(15/9) | 5 | 8 | 8 | 9 | 6 | 13 | 8 | 88 | 79 |
| CjeP I | (7/13)CCANNNNNNNTC(14/8) | 3 | 6 | 6 | 10 | 5 | 10 | 3 | 92 | 65 |
| Cla I | ATCGAT | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 15 | 2 |
| Cpo I | CGGWCCG | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 |
| CviJ I | RGCY | 39 | 44 | 51 | 73 | 69 | 109 | 102 | 692 | 680 |
| 制限酵素 | 認識配列* | 切 断 部 位 数 | ||||||||
| pKF3 | pUC19 | pUC119 | pBR322 | ColE 1 | M13mp18 | λ | Ad2 | |||
| CviR I | TGCA | 4 | 13 | 13 | 21 | 18 | 33 | 18 | 272 | 207 |
| Dde I | CTNAG | 4 | 6 | 6 | 8 | 14 | 11 | 29 | 104 | 97 |
| Dpn I | G6mATC | 12 | 15 | 15 | 22 | 0 | 19 | 7 | 116 | 87 |
| Dra I | TTTAAA | 2 | 3 | 3 | 3 | 2 | 3 | 5 | 13 | 12 |
| Dra III | CACNNNGTG | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 10 | 10 |
| Drd I | GACNNNNNNGTC | 1 | 2 | 3 | 2 | 1 | 2 | 1 | 3 | 6 |
| Drd II | GAACCA | 1 | 0 | 1 | 0 | 4 | 0 | 7 | 22 | 21 |
| Dsa I | CCRYGG | 2 | 0 | 0 | 2 | 3 | 1 | 2 | 46 | 82 |
| Eae I | YGGCCR | 2 | 3 | 3 | 6 | 2 | 3 | 3 | 39 | 70 |
| Eam1105 I | GACNNNNNGTC | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 9 | 9 |
| Eco31 I | GGTCTC(1/5) | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 2 | 18 |
| Eco52 I | CGGCCG | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 19 |
| Eco57 I | CTGAAG(16/14) | 1 | 2 | 2 | 2 | 0 | 11 | 0 | 40 | 23 |
| Eco81 I | CCTNAGG | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 7 |
| Eci I | TCCGCC | 2 | 3 | 3 | 4 | 2 | 4 | 2 | 32 | 29 |
| EcoE I | GAGNNNNNNNATGC | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 10 | 4 |
| EcoN I | CCTNNNNNAGG | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 9 | 10 |
| EcoO65 I | GGTNACC | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 13 | 10 |
| EcoO109 I | RGGNCCY | 0 | 1 | 1 | 4 | 0 | 0 | 0 | 3 | 44 |
| EcoR I | GAATTC | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 5 | 5 |
| EcoR V | GATATC | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 21 | 9 |
| EcoT14 I | CCWWGG | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 10 | 44 |
| EcoT22 I | ATGCAT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 10 |
| Esp3 I | CGTCTC(1/5) | 2 | 2 | 2 | 1 | 0 | 4 | 1 | 14 | 21 |
| Fau I | CCCGC(4/6) | 2 | 5 | 7 | 10 | 0 | 10 | 10 | 90 | 147 |
| Fba I | TGATCA | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 5 |
| Fin I | GGGAC | 1 | 0 | 0 | 4 | 2 | 10 | 2 | 38 | 59 |
| Fnu4H I | GCNGC | 16 | 19 | 22 | 42 | 31 | 45 | 17 | 380 | 411 |
| Fok I | GGATG(9/13) | 1 | 5 | 5 | 12 | 8 | 18 | 4 | 150 | 78 |
| Fse I | GGCCGGCC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
| Gdi II | YGGCCG(-5/-1) | 1 | 3 | 3 | 5 | 2 | 2 | 2 | 23 | 72 |
| Gsu I | CTGGAG(16/14) | 1 | 1 | 1 | 4 | 3 | 4 | 2 | 25 | 32 |
| Hae I | WGGCCW | 6 | 3 | 3 | 7 | 6 | 6 | 4 | 64 | 56 |
| Hae II | RGCGCY | 3 | 3 | 5 | 11 | 8 | 6 | 6 | 48 | 76 |
| Hae III | GGCC | 12 | 11 | 13 | 22 | 11 | 15 | 15 | 149 | 216 |
| Hae IV | GAYNNNNNRTC | 2 | 1 | 1 | 3 | 6 | 3 | 4 | 39 | 16 |
| Hap II | CCGG | 12 | 13 | 14 | 26 | 5 | 37 | 18 | 328 | 171 |
| Hga I | GACGC(5/10) | 2 | 4 | 4 | 11 | 14 | 15 | 7 | 102 | 87 |
| HgiA I | GWGCWC | 2 | 5 | 5 | 8 | 3 | 2 | 3 | 28 | 38 |
| HgiE II | ACCNNNNNNGGT | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 14 | 10 |
| Hha I | GCGC | 9 | 17 | 23 | 31 | 18 | 26 | 26 | 215 | 375 |
| Hin1 I | GRCGYC | 0 | 3 | 3 | 6 | 7 | 2 | 1 | 40 | 44 |
| Hinc II | GTYRAC | 3 | 1 | 1 | 2 | 13 | 0 | 1 | 35 | 25 |
| Hind III | AAGCTT | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 7 | 12 |
| Hinf I | GANTC | 4 | 6 | 8 | 10 | 21 | 15 | 27 | 148 | 72 |
| Hin4 I | GABNNNNNVTC | 4 | 2 | 2 | 5 | 9 | 6 | 5 | 59 | 52 |
| Hpa I | GTTAAC | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 14 | 6 |
| Hph I | GGTGA(8/7) | 9 | 7 | 8 | 12 | 9 | 15 | 18 | 168 | 99 |
| Kpn I | GGTACC | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 8 |
| Ksp632 I | CTCTTC(1/4) | 0 | 3 | 3 | 2 | 2 | 4 | 2 | 34 | 29 |
| Mae II | ACGT | 7 | 5 | 11 | 10 | 19 | 13 | 22 | 143 | 83 |
| Mae III | GTNAC | 6 | 11 | 13 | 17 | 17 | 24 | 24 | 156 | 118 |
| Mbo I | GATC | 12 | 15 | 15 | 22 | 0 | 19 | 7 | 116 | 87 |
| Mbo II | GAAGA(8/7) | 5 | 7 | 8 | 11 | 11 | 21 | 11 | 130 | 113 |
| Mcr I | CGRYCG | 4 | 5 | 5 | 7 | 1 | 6 | 4 | 22 | 50 |
| Mfl I | RGATCY | 4 | 7 | 7 | 8 | 0 | 4 | 3 | 21 | 22 |
| Mlu I | ACGCGT | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 7 | 5 |
| Mly I | GACTC(5/5) | 2 | 4 | 6 | 4 | 10 | 6 | 8 | 61 | 40 |
| Mme I | TCCRAC(20/18) | 3 | 2 | 3 | 4 | 5 | 3 | 4 | 18 | 25 |
| Mnl I | CCTC(7/6) | 10 | 13 | 14 | 26 | 34 | 41 | 61 | 262 | 397 |
| Mse I | TTAA | 8 | 13 | 20 | 15 | 35 | 30 | 63 | 195 | 115 |
| Msl I | CAYNNNNRTG | 1 | 3 | 3 | 7 | 7 | 3 | 2 | 62 | 35 |
| Msp I | CCGG | 12 | 13 | 14 | 26 | 5 | 37 | 18 | 328 | 171 |
| Mun I | CAATTG | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 4 |
| Mva I | CCWGG | 8 | 5 | 5 | 6 | 2 | 14 | 7 | 70 | 136 |
| Mwo I | GCNNNNNNNGC | 11 | 13 | 17 | 34 | 21 | 35 | 20 | 346 | 391 |
| Nae I | GCCGGC | 0 | 0 | 1 | 4 | 0 | 1 | 1 | 1 | 13 |
| Nco I | CCATGG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 20 |
| Nde I | CATATG | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 7 | 2 |
| Nhe I | GCTAGC | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 |
| Nla III | CATG | 6 | 11 | 11 | 26 | 22 | 26 | 15 | 181 | 183 |
| Nla IV | GGNNCC | 6 | 11 | 14 | 24 | 6 | 6 | 18 | 82 | 178 |
| Not I | GCGGCCGC | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 |
| Nru I | TCGCGA | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 5 | 5 |
| Nsb I | TGCGCA | 1 | 2 | 2 | 4 | 1 | 0 | 1 | 15 | 17 |
| Nsp I | RCATGY | 2 | 3 | 3 | 4 | 0 | 6 | 6 | 32 | 41 |
| Nsp V | TTCGAA | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 7 | 1 |
| NspB II | CMGCKG | 5 | 6 | 6 | 6 | 5 | 10 | 4 | 75 | 95 |
| Pac I | TTAATTAA | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
| 制限酵素 | 認識配列* | 切 断 部 位 数 | ||||||||
| pKF3 | pUC19 | pUC119 | pBR322 | ColE 1 | M13mp18 | λ | Ad2 | |||
| Pfl1108 I | TCGTAG | 0 | 1 | 1 | 2 | 0 | 2 | 2 | 13 | 18 |
| Ple I | GAGTC(4/5) | 2 | 4 | 6 | 4 | 10 | 6 | 8 | 61 | 40 |
| PmaC I | CACGTG | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 10 |
| Pme I | GTTTAAAC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
| PpuM I | RGGWCCY | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 23 |
| PshA I | GACNNNNGTC | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 7 | 2 |
| PshB I | ATTAAT | 1 | 3 | 3 | 1 | 2 | 0 | 7 | 17 | 3 |
| Psp1406 I | AACGTT | 2 | 2 | 3 | 4 | 3 | 0 | 2 | 7 | 3 |
| Pst I | CTGCAG | 1 | 1 | 1 | 1 | 1 | 3 | 1 | 28 | 30 |
| Pvu I | CGATCG | 1 | 2 | 2 | 1 | 0 | 2 | 1 | 3 | 7 |
| Pvu II | CAGCTG | 1 | 2 | 2 | 1 | 0 | 3 | 3 | 15 | 24 |
| RleA I | CCCACA(12/9) | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 18 | 26 |
| Sac I | GAGCTC | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 16 |
| Sac II | CCGCGG | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 33 |
| Sal I | GTCGAC | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 3 |
| SanD I | GGGWCCC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 8 |
| Sap I | GCTCTTC(1/4) | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 10 | 7 |
| Sau3A I | GATC | 12 | 15 | 15 | 22 | 0 | 19 | 7 | 116 | 87 |
| Sca I | AGTACT | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 5 | 5 |
| ScrF I | CCNGG | 13 | 12 | 12 | 16 | 3 | 32 | 11 | 184 | 233 |
| Sec I | CCNNGG | 8 | 5 | 5 | 8 | 6 | 8 | 9 | 105 | 234 |
| SexA I | ACCWGGT | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 9 |
| SfaN I | GCATC(5/9) | 3 | 8 | 8 | 22 | 12 | 26 | 7 | 169 | 86 |
| Sfe I | CTRYAG | 3 | 4 | 5 | 4 | 6 | 7 | 7 | 38 | 47 |
| Sfi I | GGCCNNNNNGGCC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
| Sgf I | GCGATCGC | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
| SgrA I | CRCCGGYG | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 6 | 6 |
| Sim I | GGGTC(-3/0) | 2 | 3 | 4 | 8 | 6 | 5 | 4 | 40 | 73 |
| Sma I | CCCGGG | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 3 | 12 |
| Smi I | ATTTAAAT | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
| Sml I | CTYRAG | 4 | 4 | 4 | 6 | 5 | 8 | 5 | 17 | 29 |
| SnaB I | TACGTA | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
| Spe I | ACTAGT | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
| Sph I | GCATGC | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 6 | 8 |
| Spl I | CGTACG | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 4 |
| Srf I | GCCCGGGC | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
| Sse8387 I | CCTGCAGG | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 5 | 3 |
| Sse8647 I | AGGWCCT | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 |
| Ssp I | AATATT | 1 | 1 | 2 | 1 | 1 | 3 | 6 | 20 | 5 |
| Sss I | CG | 147 | 173 | 210 | 329 | 267 | 361 | 329 | 3113 | 2428 |
| Stu I | AGGCCT | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 6 | 11 |
| StyLT I | CAGAG | 7 | 6 | 6 | 8 | 3 | 17 | 19 | 129 | 77 |
| StySJ I | GAGNNNNNNGTRC | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 6 | 12 |
| Taq I | TCGA | 11 | 4 | 5 | 7 | 10 | 9 | 12 | 121 | 50 |
| Taq II | GACCGA(11/9)CACCCA(11/9) | 2 | 5 | 6 | 6 | 2 | 4 | 4 | 28 | 36 |
| Tat I | WGTACW | 1 | 2 | 2 | 2 | 0 | 5 | 5 | 24 | 19 |
| Tau I | GCSGC | 9 | 7 | 9 | 21 | 17 | 11 | 7 | 181 | 232 |
| Tfi I | GAWTC | 2 | 2 | 2 | 6 | 11 | 9 | 19 | 87 | 32 |
| Tse I | GCWGC | 7 | 12 | 13 | 21 | 14 | 34 | 10 | 199 | 179 |
| TspE I | AATT | 6 | 7 | 10 | 8 | 25 | 21 | 64 | 189 | 87 |
| TspR I | CAGTG(2/-7) | 6 | 10 | 10 | 12 | 6 | 16 | 8 | 119 | 83 |
| Tsp4C I | ACNGT | 15 | 8 | 9 | 14 | 15 | 23 | 31 | 187 | 122 |
| Tsp45 I | GTSAC | 1 | 4 | 5 | 9 | 8 | 12 | 8 | 81 | 73 |
| Tth111 I | GACNNNGTC | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 12 |
| Tth111 II | CAARCA(11/9) | 3 | 3 | 3 | 5 | 11 | 8 | 7 | 48 | 53 |
| UbaD I | GAACNNNNNNTCC | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 7 | 4 |
| UbaE I | CACCTGC | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 12 | 9 |
| Van91 I | CCANNNNNTGG | 2 | 0 | 0 | 2 | 2 | 0 | 0 | 14 | 18 |
| VpaK11B I | GGWCC | 1 | 2 | 2 | 8 | 1 | 4 | 1 | 35 | 73 |
| Xba I | TCTAGA | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 5 |
| Xcm I | CCANNNNNNNNNTGG | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 14 |
| Xho I | CTCGAG | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 6 |
| Xmn I | GAANNNNTTC | 0 | 1 | 1 | 2 | 3 | 2 | 2 | 24 | 5 |
| Xsp I | CTAG | 5 | 4 | 5 | 5 | 3 | 4 | 5 | 14 | 54 |