Diversify™ PCRランダム突然変異誘発キット
使用文献
- アミロマルターゼ酵素の特性を改善するためにDiversify PCR Random Mutagenesis Kitを用いて酵素の遺伝子に変異を導入した例
Use of Random and Saturation Mutageneses To Improve the Properties of Thermus aquaticus Amylomaltase for Efficient Production of Cycloamyloses
Kazutoshi Fujii, Hirotaka Minagawa, Yoshinobu Terada, Takeshi Takaha, Takashi Kuriki, Jiro Shimada, and Hiroki Kaneko
Appl. Envir. Microbiol. (2005) Oct;71:5823-5827. - 高温下での大腸菌生育に必要なMetA遺伝子高温耐性変異体を得るためDiversify PCR Random Mutagenesis Kitを用いた例
Improved Thermostability and Acetic Acid Tolerance of Escherichia coli via Directed Evolution of Homoserine o-Succinyltransferase
Elena A. Mordukhova, Hee-Soon Lee, and Jae-Gu Pan
Appl. Envir. Microbiol. (2008) Dec;74:7660-7668.
参考文献
- Leung, D. W. et al. (1989) Technique 1:11-15.
- Cadwell, R. C. & Joyce, G. F. (1992) PCR Methods Appl. 2(1):28-33.
- Mo, J.-Y. et al. (1991) J. Mol. Biol. 222(4):925-936.
