タンパク質間相互作用をFRET-acceptor photobleaching法で解析した例(AcGFP1,DsRED-Monomerの組み合わせ)
Identification of Interaction Sites between Human βA3- and A/B-crystallins by Mammalian Two-hybrid and Fluorescence Resonance Energy Transfer Acceptor Photobleaching Methods
Ratna Gupta and Om P. Srivastava J. Biol. Chem. (2009) Jul;284:18481-18492.
AcGFP1, DsREDをミトコンドリアに局在化し発現させた例
Amyloid-β overproduction causes abnormal mitochondrial dynamics via differential modulation of mitochondrial fission/fusion proteins
Xinglong Wang, Bo Su, Sandra L. Siedlak, Paula I. Moreira, Hisashi Fujioka, Yang Wang, Gemma Casadesus, and Xiongwei Zhu PNAS, (2008) Dec;105:19318-19323.
参考文献
Gurskaya, N. G., et al., (2003) Biochem. J. 373(Pt. 2):403-408.
Reef Coral Fluorescent Protein Vectors (July 2003) ClontechniquesXVIII(3):6-7.
Matz, M. V., et al. (1999) Nature Biotechnol. 17 (10) :969-973. Erratumin: Nature Biotechnol. (1999) 17(12):1227.
Living Colors DsRed-Express (July 2003) ClontechniquesXVII(3):16.
Creator System Overview (October 2001) ClontechniquesXVI(4):5-6.