RNAiso Plus
使用文献
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J. Biol. Chem., 2008; 283: 33858 - 33864.
Sanada T., Takaesu G., Mashima R., Yoshida R., Kobayashi T., and Yoshimura A.
FLN29 Deficiency Reveals Its Negative Regulatory Role in the Toll-like Receptor (TLR) and Retinoic Acid-inducible Gene I (RIG-I)-like Helicase Signaling Pathway
使用サンプル：細胞（MEF、BMDC,HEK293T など） 目的：RT-PCR
参考文献
1) J. Chirgwin et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched
in Ribonuclease", Biochemistry. 18 (24): 5294-5299 (1979).
2) D. Wallace, “Large-and Small-Scale Phenol Extractions", Methods in Enzymology. 152: 33-41 (1987).
3) Coombs, L. M., Pigott, D., Proctor, A., Eydmann, M., Denner, J. and Knowles, M. A. “Simultaneous Isolation of DNA, RNA, and Antigenic Protein Exhibiting Kinase Activity from Small Tumor Samples Using Guanidine Isothiocyanate", Anal. Biochem. 188: 338-343 (1990).
4) Nicolaides, N. C. & Stoeckert, Jr., C. J. "A Simple, Efficient Method for the Separate Isolation of RNA and DNA from the Same Cells", Biotechniques, 8: 154-156 (1990).
5) J. R. Feramisco, et al., Molecular Cloning : 194-195, Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
6) Raha, S., Merante, F., Proteau, G. and Reed, J. K. "Simultaneous Isolation of Total Cellular RNA and DNA from Tissue Culture Cells Using Phenol and Lithium Chloride", Gene Anal. Techn. 7: 173-177 (1990).
2) D. Wallace, “Large-and Small-Scale Phenol Extractions", Methods in Enzymology. 152: 33-41 (1987).
3) Coombs, L. M., Pigott, D., Proctor, A., Eydmann, M., Denner, J. and Knowles, M. A. “Simultaneous Isolation of DNA, RNA, and Antigenic Protein Exhibiting Kinase Activity from Small Tumor Samples Using Guanidine Isothiocyanate", Anal. Biochem. 188: 338-343 (1990).
4) Nicolaides, N. C. & Stoeckert, Jr., C. J. "A Simple, Efficient Method for the Separate Isolation of RNA and DNA from the Same Cells", Biotechniques, 8: 154-156 (1990).
5) J. R. Feramisco, et al., Molecular Cloning : 194-195, Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
6) Raha, S., Merante, F., Proteau, G. and Reed, J. K. "Simultaneous Isolation of Total Cellular RNA and DNA from Tissue Culture Cells Using Phenol and Lithium Chloride", Gene Anal. Techn. 7: 173-177 (1990).
