pEcoli-Nterm 6xHN VectorにIn-Fusionを利用しクローニング後、TALONレジンで目的タンパク質を精製した例
Characterization of a novel -galactosidase activity contributing to glycoprotein degradation and virulence in Streptococcus pneumoniae
Vanessa S. Terra, Karen A. Homer, Susmitha G. Rao, Peter W. Andrew, and Hasan Yesilkaya Infect. Immun. (2009) Oct;10.1128/IAI.00721-09.
参考文献
Moffatt, B. A. & Studier, F. W. (1986) J. Mol. Biol. 189(1):113-130.