文献

In-Fusion Cloningシリーズ関連の使用文献集

タカラバイオのIn-Fusionクローニングシステムはどんなベクターのどんな位置にもディレクショナルクローニングを可能にし、クローニング効率が高く、質の高いクローニングを行える。下記様々な分野で実績があり、In-Fusionクローニングシステムを使用した文献は年々増え続けています。

• ✓ ゲノム編集 CRISPR/Cas9 Vectorのクローニング
• ✓ レンチウイルスベクターのクローニング
• ✓ ハイスループットクローニング
• ✓ マルチクローニング

１、ゲノム編集 CRISPR/Cas9 Vector のクローニング

• Li H.L., et al. (2016) Efficient genomic correction methods in human iPS cells using CRISPR–Cas9 system. Methods 101:27-35.
• Khan, et al. (2017) A highly efficient ligation‑independent cloning system for CRISPR/Cas9 based genome editing in plants. Plant Methods 13:86.
• Sakuma T., et al. (2016) MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc. 11(1):118-133.
• Jacobs, et al. (2015) Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology 15:16.

２、レンチウイルスベクターのクローニング

• Mao F., et al. (2018) Lrig1 is a haploinsufficient tumor suppressor gene in malignant glioma. Oncogenesis 7(2):13.
• Cheng Z., et al. (2017) Gene expression profiling reveals U1 snRNA regulates cancer gene expression. Oncotarget. 8(68):112867-112874.
• Dondossola, et al. (2016) Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors. Proc Natl Acad Sci U S A. 113(8):2223-2228.
• Yang K.S., et al. (2015) Single cell resolution in vivo imaging of DNA damage following PARP inhibition. Sci Rep. 5:10129.

３、ハイスループットクローニング

• Dutta D., et al. (2018) High throughput generation and characterization of replication-competent clade C transmitter-founder simian human immunodeficiency viruses. PLoS One. 13(5):e0196942.
• Spidel J.L., et al. (2016) Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells. J Immunol Methods. 439:50-58.
• Berrow N.S., et al. (2007) A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Res. 35(6):e45.

４、マルチクローニング

• Zhu B., et al. (2018) A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Biotechniques. 43(3):354-359.
• Hwang I.S., et al. (2016) Multi-Homologous Recombination-Based Gene Manipulation in the Rice Pathogen Fusarium fujikuroi. Plant Pathol J. 32(3):173-181.
• Maqbool A., et al. (2015) Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor. Elife. 4:e08709.
• Franssen H.J., et al. (2015) Root developmental programs shape the Medicago truncatula nodule meristem. Development 142: 2941-2950.
• Cohen-Rosenzweig C., et al. (2014) Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans. Appl Environ Microbiol. 80(2):486-496.
• Huang L., et al. (2014) Potential Pitfalls and Solutions for Use of Fluorescent Fusion Proteins to Study the Lysosome. PLOS ONE. 9(2): e88893.
• Emily A., et al. (2013) Identification of the N-Terminal Domain of the Influenza Virus PA Responsible for the Suppression of Host Protein Synthesis. J Virol. 87(6):3108-3118.
• Stadler L.K., et al. (2011) Structure-function studies of an engineered scaffold protein derived from Stefin A. II: Development and applications of the SQT variant. Protein Eng Des Sel. 24(9):751-763.

1. Zhu, B. et al. (2007) BioTechniques 3:354-359.
2. Marsischky, G. & LaBaer, J. (2004) Genome Res. 14:2020-2028.
3. Hartman, S. et al. (January 2005) Clontechniques XX(1):26-27.
4. Berrow, N.S. et al. (2007) Nucleic Acids Res. 35(6):e45.3.