文献

従来のRACE法を改良し、タカラ独自のSMART（Switching Mechanism at the 5' end of RNA Template）法を利用したSMARTer RACE 5'/3'Kitは下記様々な領域に実績があり、使用した文献は毎年増え続けています。

• 転写開始点の解析
• プロモーター領域の解析
• 抗体やTCR（T cell receptor）可変領域の解析
• 完全長ORF（Open Reading Frame）によるタンパク質解析
• Long non-coding RNA（LncRNA）解析

＜ 転写開始点の解析 ＞

• ◆ Ortiz de Ora L., et al. (2018) Regulation of biomass degradation by alternative σ factors in cellulolytic clostridia. Sci Rep. 8(1):11036.
• ◆ Li C., et al. (2017) A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nat Cell Biol. 19(2):106-119.
• ◆ Tahmasebi S., et al. (2016) Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2. Proc Natl Acad Sci U S A. 113(44):12360-12367.

＜ プロモーター領域の解析 ＞

• ◆ Ushijima T., et al. (2017) Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection. Cell. 171(6):1316-1325.
• ◆ Yasuda T., et al. (2016) Recurrent DUX4 fusions in B cell acute lymphoblastic leukemia of adolescents and young adults. Nat Genet. 48(5):569-74.
• ◆ Yang Y., et al. (2014) A Zinc Finger Protein Regulates Flowering Time and Abiotic Stress Tolerance in Chrysanthemum by Modulating Gibberellin Biosynthesis. Plant Cell. 26(5):2038-2054.

＜ 抗体やTCR（T Cell Receptor）可変領域の解析 ＞

• ◆ Dossa RG., et al. (2018) Development of T-cell immunotherapy for hematopoietic stem cell transplantation recipients at risk of leukemia relapse. Blood. 131(1):108-120.
• ◆ Arai Y., et al. (2018) Myeloid Conditioning with c-kit-Targeted CAR-T Cells Enables Donor Stem Cell Engraftment. Mol Ther. 26(5):1181-1197.
• ◆ Su S., et al. (2017) Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 27(4):461-482.

＜ ORFの解析 ＞

• ◆ Lv S., et al. (2018) Genetic control of seed shattering during African rice domestication. Nat Plants. 4(6):331-337.
• ◆ Zhang D., et al. (2017) Phase-specific expression of an insulin-like androgenic gland factor in a marine shrimp Lysmata wurdemanni: Implication for maintaining protandric simultaneous hermaphroditism. PLoS One. 12(2):e0172782.
• ◆ Levin S., et al. (2016) Two novel viruses associated with the Apis mellifera pathogenic mite Varroa destructor. Sci Rep. 6:37710.

＜ LncRNAの解析 ＞

• ◆ Zemmour D., et al. (2017) Flicr, a long noncoding RNA, modulates Foxp3. Proc Natl Acad Sci U S A. 114(17):E3472-E3480.
• ◆ Lagarde J., et al. (2016) Extension of human lncRNA transcripts by RACE coupled with long-read high-throughput sequencing (RACE-Seq). Nat Commun. 7:12339.
• ◆ Sun M., et al. (2016) LncRNA HOXA11-AS promotes proliferation and invasion of gastric cancer by scaffolding the chromatin modification factors PRC2, LSD1 and DNMT1. Cancer Res. 76(21):6299-6310.