TaKaRa Taq、TaKaRa Ex Taq、TaKaRa LA Taqを用いた増幅例
λDNA増幅例
| Lane A: |
pHY Marker |
7: |
10 kb |
| 1: |
0.5 kb |
8: |
12 kb |
| 2: |
1 kb |
9: |
15 kb |
| 3: |
2 kb |
10: |
20 kb |
| 4: |
4 kb |
11: |
28 kb |
| 5: |
6 kb |
12: |
35 kb |
| 6: |
8 kb |
B: |
λ-Hind III Marker |
ヒトゲノム増幅例
| Lane A: |
pHY Marker |
7: |
28.4 kb |
| 1: |
0.262 kb |
8: |
29.9 kb |
| 2: |
2.9 kb | 9: |
30.8 kb |
| 3: |
8.5 kb |
A: |
pHY Marker |
| 4: |
17.5 kb |
B: |
λ-Hind III Marker |
| 5: |
23.2 kb |
|
| 6: |
27 kb |
|
【PCR条件】
λDNA増幅例
| Lane 1-3 : |
94℃, 1min. |
|
| |
98℃, 5 sec. |
30 cycles |
| |
68℃, 5 min. |
| |
72℃, 10 min. |
|
ヒトゲノム増幅例
| Lane 1 : |
94℃, 1min. |
|
| |
94℃, 30 sec. |
|
| |
58℃, 1 min. |
30 cycles |
| |
72℃, 1 min. |
| |
72℃, 10 min. |
|
| Lane 3-9 : |
94℃, 1 min. |
|
| |
98℃, 5 sec. |
30 cycles |
| |
68℃, 15 min. |
| |
72℃, 10 min. |
|
| Lane 4-12 : |
94℃,1min. |
|
| |
98℃, 5 sec. |
30 cycles |
|
| |
68℃, 15 min. |
| |
72℃,10 min. |
|
| Lane 2 : |
94℃,1min. |
|
| |
94℃, 30 sec. |
|
| |
58℃, 1 min. |
30 cycles |
|
| |
72℃, 3 min. |
| |
72℃,10 min. |
|
TaKaRa Ex Taq®